Trichoderma Harzianum Enhances Resistance to Botrytis Cinerea by Promoting Photosynthesis, Redox Homeostasis and Secondary Metabolites in Tomato

Chen, Shuangchen; Fu, Yalun; Peng, Xiaohua; Yang, Zhengkun; Zhang, Mengke; Geng, Lijiahong; Song, Zhiyu; Xu, Qi; Chen, Junliang; Song, Shuang; Liu, Airong; Ahammed, Golam Jalal

doi:10.2139/ssrn.3990631

Cited by 1 publication

(1 citation statement)

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“…Host defence activation following B. cinerea infection is accompanied by massive transcriptional reprogramming (Windram et al 2012 ; De Cremer et al 2013 ), notably causing changes in the expression of metabolism-associated genes, most commonly the repression of photosynthesis-associated genes and the activation of genes involved in defence secondary pathways, including phenolics, these responses being confirmed by direct measurements in tomato (Chen et al 2021 ). Metabolomic studies are also in line with these findings (Camañes et al 2015 ; Gao et al 2021 ), highlighting the role of phenolic compounds following B. cinerea infection, notably the accumulation of hydroxycinnamic acids such as ferulic and caffeic acids, which are precursors of lignins and cross-linkers of cell wall polysaccharides.…”

Section: Introductionmentioning

confidence: 98%

Nitrogen-mediated metabolic patterns of susceptibility to Botrytis cinerea infection in tomato (Solanum lycopersicum) stems

Lacrampe

Colombié

Dumont

et al. 2023

Planta

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Main conclusion Severe N stress allows an accumulation of C-based compounds but impedes that of N-based compounds required to lower the susceptibility of tomato stem toBotrytis cinerea. Abstract Botrytis cinerea, a necrotrophic filamentous fungus, forms potentially lethal lesions on the stems of infected plants. Contrasted levels of susceptibility to B. cinerea were obtained in a tomato cultivar grown on a range of nitrate concentration: low N supply resulted in high susceptibility while high N supply conferred a strong resistance. Metabolic deviations and physiological traits resulting from both infection and nitrogen limitation were investigated in the symptomless stem tissue surrounding the necrotic lesion. Prior to infection, nitrogen-deficient plants showed reduced levels of nitrogen-based compounds such as amino acids, proteins, and glutathione and elevated levels of carbon-based and defence compounds such as α-tomatine and chlorogenic acid. After B. cinerea inoculation, all plants displayed a few common responses, mainly alanine accumulation and galactinol depletion. The metabolome of resistant plants grown under high N supply showed no significant change after inoculation. On the contrary, the metabolome of susceptible plants grown under low N supply showed massive metabolic adjustments, including changes in central metabolism around glutamate and respiratory pathways, suggesting active resource mobilization and production of energy and reducing power. Redox and defence metabolisms were also stimulated by the infection in plants grown under low N supply; glutathione and chlorogenic acid accumulated, as well as metabolites with more controversial defensive roles, such as polyamines, GABA, branched-chain amino acids and phytosterols. Taken together, the results showed that nitrogen deficiency, although leading to an increase in secondary metabolites even before the pathogen attack, must have compromised the constitutive levels of defence proteins and delayed or attenuated the induced responses. The involvement of galactinol, alanine, cycloartenol and citramalate in the tomato stem response to B. cinerea is reported here for the first time.

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