2023
DOI: 10.1016/j.biocontrol.2023.105281
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Trichoderma harzianum marker-free strain construction based on efficient CRISPR/Cas9 recyclable system: A helpful tool for the study of biological control agents

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Cited by 3 publications
(3 citation statements)
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“…A pioneering study demonstrated the modification of Trichoderma harzianum using the CRISPR/Cas9 marker-free system, targeting the albA (pks4) and ku70 genes. This achievement marks the first successful application of this recyclable system for constructing fungal strains with agricultural applications [33].…”
Section: Generations Biofuel Technology [23] Bio-input Technology Adv...mentioning
confidence: 82%
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“…A pioneering study demonstrated the modification of Trichoderma harzianum using the CRISPR/Cas9 marker-free system, targeting the albA (pks4) and ku70 genes. This achievement marks the first successful application of this recyclable system for constructing fungal strains with agricultural applications [33].…”
Section: Generations Biofuel Technology [23] Bio-input Technology Adv...mentioning
confidence: 82%
“…The study successfully developed two strains of T. harzianum using a recyclable CRISPR/Cas9 marker-free system based on the AMA1 plasmid vector. This achievement marks the first successful application of this recyclable system for constructing fungal strains with agricultural applications [33].…”
Section: A Simple and Inexpensive Techniquementioning
confidence: 82%
“…Due to this, there is great interest in improving these strains for industrial applications in increasing biomass and biofuel production (Martinez et al, 2008). Vector expression Loss of pigmentation of the conidia due to the disruption of the pks4 and ku70 genes involved in melanin synthesis Cortes et al, 2023 Biolistic transformation Mutants obtained with an auxotrophic marker, phenotypic characterization demonstrated a reduction in the growth of the mutants in the absence of uridine, as well as resistance to 5-fluorotic acid Vieira et al, 2021 Furthermore, although the T. reesei genome has been sequenced (Li et al, 2017), little-studied cellular functions are essential in different gene biological pathways. However, despite the multiple utilities of this fungus, some factors limit the production of these enzymes.…”
Section: Genetic Improvement Of Trichodermamentioning
confidence: 99%