Geologically recent, Pantanal presents the biodiversity merging Amazon and Cerrado. Few studies on macrofauna were done in this region, leaving a great hole about the phytophile composition. Furtherlmore, the great abundance of macrophytes, such as Eichhornia azurea which forms huge beds and is present in the lentic systems of the Pantanal, has the role of also host organisms of the macrofauna community, which can suffer with any hidrological cycle changes. Therefore, we conducted a study aiming to identify the phytophile macrofauna associated to E. azurea, from different bays and hydrologic periods in Pantanal. Samples were taken monthly containing stem and roots of the macrophytetogether with limnological data of dissolved oxygen, pH, temperature and transparency. The macroinvertebrates associated to the macrophyte were screened to the family taxonomic level in order to obtain the diversity index (Shannon-Wiener), homogeneity and Biological Monitoring Working Party (BMWP) for biomonitoring. In total, 4,164 specimens belonging to 42 families in 12 large groups were identified. Chironomidae (Diptera) was the most abundant family in all of the sample sites and seasons. The Shannon-Wienner index varied from 0.94 to 1.08 and the BMWP indicated that the systems presented good water quality in all of the studied bays. The macrofauna diversity changes according to the season, indicating that the community structure also depends on the environmental parameters. Therefore, the uses of biomonitoring indexes in order to obtain the environmental quality of the bays in the Pantanal denotes that the organisms hosted by macrophytes can also be a tool to generate results for the environmental analysis.