Trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins

Hw, Yeung; Ww, Li; Zhang, Feng

doi:10.1016/0378-8741(88)90102-x

Cited by 12 publications

(14 citation statements)

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“…In this study, we observed that the aqueous extract of Momordica charantia completely lost its inhibitory activity against Candida albicans and Cryptococcus neoformans after fractionation. This activity is probably due to some extent to proteins, known to have hiperglycaemic, antitumor, antileukemic and antiviral properties, such as lectins (MAP 30), napin-like ribosome inactivating peptides (charantin), trypsin inhibitors (MCTI-I, -II, -III, ␣-momorcharin and ␤-momorcharin) and elastase inhibitors (MCEI-I, -II, -III and -IV) (Yeung et al, 1988;Hara et al, 1989;Hamato et al, 1995;Mors et al, 2000;Arazi et al, 2002;Parkash et al, 2002;Rathi et al, 2002) present in the aqueous extact of Momordica charantia and lost after separation of the precipitate from the supernatant. Thus, if the total aqueous extract shows activity, that activity comes from the precipitate, since the supernatant does not show the same activity.…”

Section: Resultsmentioning

confidence: 99%

Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants

Schmourlo

Mendonça-Filho

Alviano

et al. 2005

Journal of Ethnopharmacology

161

103

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Section: Resultsmentioning

confidence: 99%

Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants

Schmourlo

Mendonça-Filho

Alviano

et al. 2005

Journal of Ethnopharmacology

161

103

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“…RIPs have been reported in approximately 17 different plant families, including Cucurbitaceae, Poaceae, and Euphorbiaceae (Sharma et al, 2004). RIPs show some properties including antibacterial (Pelegrini et al, 2008), antifungal (Parkash et al, 2002), and a multiplicity of antitumor (Cao et al, 2012;Shin et al, 2013), abortifacient (Yeung et al, 1988), and antiviral (for example, polio virus, cytomegalo virus, influenza virus, herpes simplex virus, and anti-human immunodeficiency virus (HIV)) activities Park et al, 2006;Au et al, 2014). RIPs have attracted a lot of attention in biomedical research, especially immunotoxins in targeted therapy studies (Battelli et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Cloning and soluble expression of mature α-luffin from Luffa cylindrica inE. coli using SUMO fusion protein

et al. 2018

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α-Luffin, found in Luffa cylindrica seeds, is a type I ribosome inactivating proteins. Cytotoxic effects make it an appropriate candidate for the construction of immunotoxins and conjugates. Because of limited natural resources, recombinant technology is the best approach to achieve large-scale production of plant-based proteins. In the present study, α-luffin protein was expressed in E. coli and the effects of different temperature conditions, SUMO fusion tag, and cultivation strategies on total expression and solubility were investigated. Protein expression was evaluated at different intervals (0, 4, 6, 8, 24 h) postinduction. Our results showed that EnBase had higher efficiency than LB, and maximum solubility and total protein expression were achieved 24 h after induction at 30 °C and 25 °C, respectively. It was shown that SUMO tag is an effective strategy to improve protein solubility.

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“…Thus, holotoxins are highly toxic to cells expressing receptors for a B subunit(s), but not to receptor-deprived cells, and are not toxic to normal cells as isolated A chains (5,14,24). Plant hemitoxins are not toxic to plants that synthesize them and have low cytotoxicity against animal cells, unless the cells have high pinocytic activity (11,59). However, these hemitoxins can enter and eliminate virally infected plant cells (4) and some are also found to be highly toxic to various virally infected animal cells (24).…”

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confidence: 99%

Antiviral Activity of Shiga Toxin Requires Enzymatic Activity and Is Associated with Increased Permeability of the Target Cells

et al. 2003

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This study expanded our earlier finding that Shiga toxin type 1 (Stx1) has activity against bovine leukemia virus (BLV) (W. A. Ferens and C. J. Hovde, Infect. Immun. 68:4462-4469, 2000). The Stx molecular motifs required for antiviral activity were identified, and a mechanism of Stx action on virally infected cells is suggested. Using inhibition of BLV-dependent spontaneous lymphocyte proliferation as a measure of antiviral activity, we showed that Stx2 had antiviral activity similar to that of Stx1. Enzymatic and antiviral activities of three StxA1 chain mutants deficient in enzymatic activity or aspects of receptor-mediated cytotoxicity were compared. Using protein synthesis inhibition to measure enzymatic activity, the mutant E167D was 300-fold less catalytically active than wild-type StxA1, was minimally active in antiviral assays, and did not inhibit synthesis of viral proteins. Two StxA1 mutants, A231D-G234E and StxA 1 1 (enzymatically active but unable to kill cells via the classical receptor-mediated route), had undiminished antiviral activity. Although binding of radiolabeled StxA1 to bovine blood cells or to free virus was not detected, flow cytometric analysis showed that the number of BLV-expressing cells were specifically reduced in cultures treated with Stx. These unique and rare lymphocytes were highly permeable to 40-and 70-kDa fluorescent dextrans, indicating that direct absorption of toxins by virus-expressing cells is a potential mechanism of target cell intoxication. These results support the hypothesis that Stx-producing Escherichia coli colonization of the gastrointestinal tract may benefit ruminant hosts by the ability of Stxs to exert antiviral activity.Ruminant animals are a reservoir for Shiga toxin (Stx)-producing Escherichia coli (STEC) that can cause hemorrhagic colitis and life-threatening sequelae in humans (15,23,25). STEC are part of the normal ruminant gastrointestinal microbiota and are frequently isolated from cattle, sheep, and deer (8,38,41,45). Surveys of healthy domesticated cattle routinely show a high prevalence of STEC in animals around the world (7,8,10,12,(34)(35)(36). The reasons for the wide distribution of STEC in ruminants are not known. The possible benefits arising from gastrointestinal tract colonization by STEC include postulated enhancement of gastrointestinal mucosal architecture

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Trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins

Cited by 12 publications

References 0 publications

Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants

Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants

Cloning and soluble expression of mature α-luffin from Luffa cylindrica inE. coli using SUMO fusion protein

Antiviral Activity of Shiga Toxin Requires Enzymatic Activity and Is Associated with Increased Permeability of the Target Cells

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