Trichosanthin inhibits cervical cancer by regulating oxidative stress-induced apoptosis

Abstract

Based on many studies, trichosanthin (TCS) has an antiviral effect that regulates immune response, and targets cancer cells to exert broad-spectrum anti-tumor pharmacological activities. It is speculated that TCS may be a potential natural active drug for preventing as well as treating cervical cancer. But the clearer impact along with underlying TCS mechanism on cervical cancer are still unclear. The purpose of this study is to investigate the function and potential mechanism of TCS in cervical cancer. We measured the viability of cervical cancer cell lines (HeLa & caski cells) using CCK-8 analysis, detected cell proliferation efficiency through Ki-67 staining, analyzed cell apoptosis rate via flow cytometry as well as annexin V-FITC/PI double staining, performed apoptosis-related protein expression through western blotting, evaluated cell migration along with invasion by wound as well as transwell assays, carried out MMP via JC-1 and Rh123 fluorescent probes, as well as detected intracellular ATP and ROS levels by flow cytometry, respectively, to evaluate the effects of TCS. We found that TCS inhibited viability along with proliferation, induced apoptosis, as well as inhibited HeLa & caski cell migration along with invasion in a time-and dose-dependent manner. Additionally, TCS also reduced MMP, and the production of adenosine triphosphate, as well as induced the increase of intracellular reactive oxygen species in cancer cell lines. In accordance with the present studies, TCS inhibits HeLa & caski cell proliferation along with migration but promotes their apoptosis, which may be mediated by regulating oxidative stress.

Overexpressing long non-coding RNA OIP5-AS1 ameliorates sepsis-induced lung injury in a rat model via regulating the miR-128-3p/Sirtuin-1 pathway

“…Following cell cultivation in the dark at 4°C for 30 min, the Invitrogen Attune NXT flow cytometry (Catalog. number was A24863, Thermo Fisher Scientific, Shanghai, China) was manipulated to monitor apoptosis [ 27 ]. Through computer algorithm processing, the percentage of apoptosis was worked out.…”

CircRNA protein tyrosine phosphatase receptor type a suppresses proliferation and induces apoptosis of lung adenocarcinoma cells via regulation of microRNA-582-3p

“…Previous reports have confirmed that miR-582-3p contributes to poor prognosis of NSCLC patients by activating the Wnt/β-catenin signaling pathway [ 37 ]. In our report, to explore the role of miR-582-3p in A549 cells, miR-582-3p inhibitor or inhibitor control were transfected into A549 cells.…”

“…no. KA3805; Abnova) was used to detect apoptosis according to the manufacturer’s instructions ( https://www.abcam.com/protocols/annexin-v-detection-protocol-for-apoptosis ) [ 37 ].…”

Bupivacaine modulates the apoptosis and ferroptosis in bladder cancer via phosphatidylinositol 3-kinase (PI3K)/AKT pathway

“…MMP was measured by JC-1 method as previous study [ 26 ]. Briefly, the cells were seeded on 6-well plates (2 × 10 5 cells/well) and cultured overnight at 37°C.…”