Trichostatin A Modulates Apoptotic-Related Gene Expression and Improves Embryo Viability in Cloned Bovine Embryos

Cui, Xiang-Shun; Xu, Yong‐Nan; Shen, Xiaopei; Zhang, Liqun; Zhang, Jiabao; Kim, Nam‐Hyung

doi:10.1089/cell.2010.0060

Cited by 54 publications

(39 citation statements)

References 72 publications

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“…The level of apoptosis is an important criterion to evaluate the quality of embryos because apoptotic cell death plays an important role in embryo development by eliminating cells with nuclear or chromosomal abnormalities. The positive correction has been seen between incidence of apoptosis and reprogramming of donor cell in SCNT embryos [48]. We found higher TUNEL labeling in SCNT embryos compared with IVF and parthenogenetic embryos, and this incidence was much higher than blastocysts produced from TSAtreated donor cells, which was comparable with that of IVF counterpart.…”

Section: Discussionsupporting

confidence: 62%

Trichostatin A alters the expression of cell cycle controlling genes and microRNAs in donor cells and subsequently improves the yield and quality of cloned bovine embryos in vitro

Saini

Selokar

Revey³

et al. 2014

Theriogenology

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Section: Discussionsupporting

confidence: 62%

Trichostatin A alters the expression of cell cycle controlling genes and microRNAs in donor cells and subsequently improves the yield and quality of cloned bovine embryos in vitro

Saini

Selokar

Revey³

et al. 2014

Theriogenology

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“…coding genes in cloned embryos (15). One recent report suggests that microRNA reprogramming after SCNT is incomplete and inconsistent in elongating bovine embryos (13).…”

Section: Discussionmentioning

confidence: 99%

“…Experiments using artificially inseminated gilts demonstrate that early cleavage-stage development and blastocyst formation (until day 7 of development) in pigs is virtually 100% (30,57). Therefore, the vast majority of early embryonic loss is likely to occur between day 7 and day [15][16], when embryonic attachment begins. Proper development of porcine embryos during this peri-attachment period involves dramatic morphological and structural as well as functional transformations, changes collectively referred to as "elongation," as well as the emergence of two additional germ layers: endoderm and mesoderm.…”

mentioning

confidence: 99%

Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies

Isom

Stevens

et al. 2013

Physiological Genomics

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Isom SC, Stevens JR, Li R, Spollen WG, Cox L, Spate LD, Murphy CN, Prather RS. Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies. Physiol Genomics 45: 577-589, 2013. First published May 21, 2013 doi:10.1152/physiolgenomics.00094.2012.-Substantial mortality of in vitro manipulated porcine embryos is observed during peri-attachment development. Herein we describe our efforts to characterize the transcriptomes of embryonic disc (ED) and trophectoderm (TE) cells from porcine embryos derived from in vivo fertilization, in vitro fertilization (IVF), parthenogenetic oocyte activation (PA), and somatic cell nuclear transfer (SCNT) on days 10, 12, and 14 of gestation. The IVF, PA, and SCNT embryos were generated with in vitro matured oocytes and were cultured overnight in vitro before being transferred to recipient females. Sequencing of cDNA from the resulting embryonic samples was accomplished with the Genome Analyzer IIx platform from Illumina. Reads were aligned to a custom-built swine transcriptome. A generalized linear model was fit for ED and TE samples separately, accounting for embryo type, gestation day, and their interaction. Those genes with significant differences between embryo types were characterized in terms of gene ontologies and KEGG pathways. Transforming growth factor-␤ signaling was downregulated in the EDs of IVF embryos. In TE cells from IVF embryos, ubiquitin-mediated proteolysis and ErbB signaling were aberrantly regulated. Expression of genes involved in chromatin modification, gene silencing by RNA, and apoptosis was significantly disrupted in ED cells from SCNT embryos. In summary, we have used high-throughput sequencing technologies to compare gene expression profiles of various embryo types during peri-attachment development. We expect that these data will provide important insight into the root causes of (and possible opportunities for mitigation of) suboptimal development of embryos derived from assisted reproductive technologies.in vitro fertilization; parthenogenesis; somatic cell nuclear transfer; in vitro embryo production; Illumina; ART IN MOST MAMMALIAN SPECIES studied to date, only 50 -70% of successfully fertilized oocytes will give rise to live, healthy offspring (reviewed in Refs. 20,57,79). Some embryos succumb to the effects of chromosomal abnormalities or faulty uterine environments, for example. But the underlying cause behind most early embryonic mortality is still poorly understood. Such inefficiency is concerning to human fertility clinical practitioners and to animal producers in agricultural settings as well. Embryos produced by assisted reproductive technologies (ART) are generally less fit and more prone to failure than in vivo produced embryos. It has been presupposed that oocyte maturation and embryo culture systems that are inadequate to promote proper development are primarily to blame, yet the precise mechanisms that contribute to deficiencies in developmental potential in in vitr...

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“…However, no difference was observed in the expression level of CASPASE-3, a proapoptotic gene among the three groups. The lowering of apoptotic index is particularly important for SCNT embryos because the higher apoptotic morphology and/or TUNEL labeling reported in SCNT bovine embryos compared to that in IVF embryos (Cui et al, 2011;Selokar et al, 2013) may be one of the important causes of the lower conception rate obtained with the former. The in vivo developmental competence of bovine SCNT-derived BCB + blastocysts has been shown to be higher than that of BCB -blastocysts in terms of live offspring rate in a recent study (Su et al, 2012).…”

Section: Oocyte Selection For Scnt By Bcb Staining 145mentioning

confidence: 99%

Buffalo Embryos Produced by Handmade Cloning from Oocytes Selected Using Brilliant Cresyl Blue Staining Have Better Developmental Competence and Quality and Are Closer to Embryos Produced byIn VitroFertilization in Terms of Their Epigenetic Status and Gene Expression Pattern

Mohapatra

Sandhu

Neerukattu

et al. 2015

Cellular Reprogramming

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We compared handmade cloned (HMC) buffalo blastocysts produced from oocytes stained with Brilliant Cresyl Blue (BCB) and classified into those with blue (BCB + ) or colorless cytoplasm (BCB -). The blastocyst rate was higher ( p < 0.001) for BCB + than for BCB -oocytes (43.41 -2.54 vs. 22.74 -1.76%). BCB + blastocysts had inner cell mass (ICM) cell number, ICM-to-trophectoderm ratio, global level of H3K18ac, apoptotic index, and expression level of BCL-XL, but not that of CASPASE-3, similar to that of blastocysts produced through in vitro fertilization (IVF), which was higher ( p < 0.05) than that of BCB -blastocysts. The global level of H3K9me2, which was similar in BCB + and BCB -blastocysts, was higher ( p < 0.01) than that in IVF blastocysts. The expression level of OCT4 and SOX2 was higher ( p < 0.05) and that of GATA2 was lower ( p < 0.05) in BCB + than that in BCB -blastocysts, whereas that of DNMT1, DNMT3a, NANOG, and CDX2 was not significantly different between the two groups. The expression level of DNMT1, OCT4, NANOG, and SOX2 was lower ( p < 0.05) and that of CDX2 was higher ( p < 0.05) in BCB + than in IVF blastocysts. In conclusion, because BCB + blastocysts have better developmental competence and are closer to IVF blastocysts in terms of quality, epigenetic status, and gene expression than BCB -blastocysts, BCB staining can be used effectively for selection of developmentally competent oocytes for HMC.

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Trichostatin A Modulates Apoptotic-Related Gene Expression and Improves Embryo Viability in Cloned Bovine Embryos

Cited by 54 publications

References 72 publications

Trichostatin A alters the expression of cell cycle controlling genes and microRNAs in donor cells and subsequently improves the yield and quality of cloned bovine embryos in vitro

Trichostatin A alters the expression of cell cycle controlling genes and microRNAs in donor cells and subsequently improves the yield and quality of cloned bovine embryos in vitro

Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies

Buffalo Embryos Produced by Handmade Cloning from Oocytes Selected Using Brilliant Cresyl Blue Staining Have Better Developmental Competence and Quality and Are Closer to Embryos Produced byIn VitroFertilization in Terms of Their Epigenetic Status and Gene Expression Pattern

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