Trichostrongylus and Haemonchus anthelmintic resistance in naturally infected sheep from southern Brazil

Klauck, Vanderlei; Pazinato, Rafael; Lopes, Leandro Sâmia; Cucco, D. C.; Lima, Horácio Luis de; Volpato, Andréia; Radavelli, Willian M.; Stefani, Lenita C M; Silva, Aleksandro S. Da

doi:10.1590/0001-3765201420130061

Cited by 9 publications

(5 citation statements)

References 22 publications

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“…This study revealed multiple infections in cattle in the Adamawa region of Cameroon and this parasitism was similar to that already reported in cattle in many countries in Africa including Burkina Faso [22], Senegal [18] and Cameroon in small ruminants [9]. Similar results with the predominance of Haemonchus and Trichostrongylus genera were also obtained in sheep in Brazil by Klauck et al, [23]. The antagonistic variation between Trichostrongylus sp and Haemonchus could be due to ecological niche competition as the two parasites share the same habitat (abomasum) [24].…”

Section: Variation Of the Larval Population Of Strongylessupporting

confidence: 90%

Efficacy of Albendazole on Gastro-Intestinal Strongyles of Cattle in Ngaoundere (Adamawa-Cameroon)

2018

Integr J Vet Biosci

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The present study aimed at identifying the gastro-intestinal Strongyles of cattle and to evaluate the effect of albendazole on their population dynamics throughout the year. Three cattle farms located in the village of Velambai were used for the study. 175 animals were screened through coprological examination to assess their infection rate (IR) with gastro-intestinal nematodes. 50 Goudali were monitored from April 2015 to March 2016. Of these animals selected, 25 of them received albendazole on day 0 (D0) and constituted the treated group while the other 25 received nothing and stood for the non-treated group. Faeces were examined using the McMaster method to evaluate the efficacy of treatment and to monitor changes in faecal shedding of Strongyles. Faeces from animals were cultured to recover the infective L3 larval stage of Strongyles by the Baermann method. The survey revealed that Strongyles, Strongyloides and Toxocara were the main gastro-intestinal helminths infecting cattle with IRs of 64.5%, 15% and 24.1% respectively. Deworming at the beginning of the rainy season reduced the shedding of helminth eggs (EPG < 400) throughout the season. Percentage reduction in the number of eggs per gram of faeces (EPG) from Strongyles was 68% at day 30. Stool culture revealed the presence of four types of Strongyles with varying abundance depending on the genus (Trichostrongylus, Haemonchus, Cooperia and Oesophagostomum), animal group and month. The genera Trichostrongylus and Haemonchus were dominant throughout the year. Haemonchus spp were significantly sensitive to albendazole between D30 to D90. On D120, albendazole lost its effect with the genus Haemonchus which resulted in the re-infection of the animals, whereas this effect was rather late for the genus Oesophagostomum (from D270).

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Section: Variation Of the Larval Population Of Strongylessupporting

confidence: 90%

Efficacy of Albendazole on Gastro-Intestinal Strongyles of Cattle in Ngaoundere (Adamawa-Cameroon)

2018

Integr J Vet Biosci

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“…This method helps to reduce the parasitic burden in the animals, but it has some disadvantages, including the imminent emergence of anthelmintic resistance in the parasites (MÁRQUEZ-LARA, 2003;FORTES et al, 2013). This situation a serious problem to animal health because it diminishes the efficacy of anthelmintics, and new reports of anthelmintic resistance are being recorded every year (KLAUCK et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Predatory activity of Butlerius nematodes and nematophagous fungi against Haemonchus contortus infective larvae

Silva

Uriostegui

Millán-Orozco

et al. 2017

Rev. Bras. Parasitol. Vet.

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The purpose of this study was to evaluate the predatory activity of the nematode Butlerius spp. and fungal isolates of Duddingtonia flagrans, Clonostachys rosea, Arthrobotrys musiformis and Trichoderma esau against H. contortus infective larvae (L 3 ) in grass pots. Forty-eight plastic gardening pots containing 140 g of sterile soil were used. Panicum spp. grass seeds (200 mg) were sown into each pot and individually watered with 10 mL of tap water. Twelve days after seeding, the pots were randomly divided into 6 groups (n=8

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“…In a previous study, high occurrence of multidrug resistance in 30 sheep flocks from São Paulo state was observed, and levamisole was the most efficient anthelmintic (Veríssimo et al 2012). Levamisole is an imidazothiazole widely used to control nematodes in ruminants (Fauvin et al 2010;Klauck et al 2014). So, the implementation of strategies to control gastrointestinal nematodes with levamisole to preserve its efficacy for longer periods is important, through developing and validating tests to detect resistance to levamisole.…”

Section: Introductionmentioning

confidence: 99%

Target selected treatment with levamisole to control the development of anthelmintic resistance in a sheep flock

et al. 2015

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Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of this nematode control scheme was evaluated after 5 years by FECRT, larval development test (LDT), and a molecular test to assess the development of levamisole resistance in Haemonchus contortus. Animals were submitted to treatments with albendazole, levamisole, closantel, ivermectin, moxidectin, and monepantel. Eggs per gram of feces (EPG) counts and fecal cultures were performed, and anthelmintic efficacy was calculated by the RESO 4.0 program. The helminths of the flock (GIN Embrapa2014) were compared to susceptible (McMaster) and resistant (Embrapa2010) H. contortus isolates in the LDT to estimate the LC50 and LC90 of levamisole and in a molecular test to evaluate the 63-bp indel in the acr8 gene associated with levamisole resistance. In the FECRT, parasites were susceptible to monepantel (99.6 %) and closantel (98.3 %), but resistant to moxidectin (93.8 %), levamisole (70.4 %), ivermectin (48.1 %), and albendazole (0 %). In the coproculture on D14, and the control group presented 80 % H. contortus and 20 % Trichostrongylus sp., while in the monepantel group L1 were observed as well as Oesophagostomum sp. L3. LDT and resistance factors provided good separation between susceptible and resistant parasites. The genotypic frequencies of the 63-bp insertion in the acr8 gene in H. contortus were 11.9, 6.7, and 0 % in GIN Embrapa2014, Embrapa2010, and McMaster isolates, respectively. After 5 years of exclusive use, the nematodes developed resistance to levamisole, detected by FECRT and by increase in LC50 and LC90 for levamisole in the LDT. The 63-bp indel was not confirmed as a molecular marker of levamisole resistance in our isolates. The target selected treatment scheme was effective to control helminths in the sheep flock for 5 years, when levamisole's inefficacy was perceived because of no change in the clinical situation of treated animals. Through this scheme, it was possible to promote reversion towards susceptibility or increase of efficacy for other chemical classes. Thus, this is a valid recommendation to control worms and to delay the development of resistance, preserving other anthelmintic classes for future use.

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Trichostrongylus and Haemonchus anthelmintic resistance in naturally infected sheep from southern Brazil

Cited by 9 publications

References 22 publications

Efficacy of Albendazole on Gastro-Intestinal Strongyles of Cattle in Ngaoundere (Adamawa-Cameroon)

Efficacy of Albendazole on Gastro-Intestinal Strongyles of Cattle in Ngaoundere (Adamawa-Cameroon)

Predatory activity of Butlerius nematodes and nematophagous fungi against Haemonchus contortus infective larvae

Target selected treatment with levamisole to control the development of anthelmintic resistance in a sheep flock

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