Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina

Castañares, Eliana; Albuquerque, Diana Ramírez; Dinolfo, María Inés; Pinto, Virginia Fernández; Patriarca, Andrea; Stenglein, Sebastián Alberto

doi:10.1016/j.ijfoodmicro.2014.03.024

Cited by 57 publications

(61 citation statements)

References 34 publications

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“…In Argentina, although 15-ADON seems to dominate among F. graminearum s.s. isolates, a DON/NIV genotype/chemotype has also been found in wheat and barley. The DON/NIV genotype seems to occur at very low frequencies in populations of F. graminearum and its chemical phenotype was either DON or NIV (Reynoso et al, 2011;Castañares et al, 2014). Nevertheless, the DON/NIV genotype was not detected in the current study.…”

Section: Discussioncontrasting

confidence: 77%

“…While trichothecene genotypes refer to PCR-based analyses of the Tri genes, chemotypes refer to chemical-based analyses (Desjardins, 2008). However, due to discrepancies between genotypes and chemical data reported previously, both types of analysis are necessary to define the profile of trichothecenes that F. graminearum isolates synthesize (Mugrabi de Kuppler et al, 2011;Castañares et al, 2014;Somma et al, 2014). Furthermore, chemical profile surveys of F. graminearum s.s. isolated from wheat have not been previously carried out in Uruguay.…”

Section: Introductionmentioning

confidence: 99%

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Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay

et al. 2016

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Section: Discussioncontrasting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay

et al. 2016

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“…In southern of Europe the predominant 15-ADON genotype is more predominant (Somma et al, 2014); however, we used a F. graminearum strain from Argentina. Castañares et al (2014) showed predominant presence of 3-ADON phenotypic Fusarium species in Argentina than in Europe. No studies exist on 15-ADON thermal stability; however, De Boevre et al…”

Section: Fate Of Don Conjugates During Baking (Further Analysed With mentioning

confidence: 95%

Thermal stability and kinetics of degradation of deoxynivalenol, deoxynivalenol conjugates and ochratoxin A during baking of wheat bakery products

et al. 2015

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The stability of deoxynivalenol (DON), deoxynivalenol-3-glucoside (DON-3-glucoside), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), de-epoxy-deoxynivalenol (DOM-1) and ochratoxin A (OTA) during thermal processing has been studied. Baking temperature, time and initial mycotoxin concentration in the raw materials were assayed as factors. An improved UPLC-MS/MS method to detect DON, DON-3-glucoside, 3-ADON, 15-ADON and DOM-1 in wheat baked products was developed in the present assay. The results highlighted the importance of temperature and time in mycotoxin stability in heat treatments. OTA is more stable than DON in a baking treatment. Interestingly, the DON-3-glucoside concentrations increased (>300%) under mild baking conditions. On the other hand, it was rapidly reduced under harsh conditions. The 3-ADON decreased during the heat treatment; while DOM-1 increased after the heating process. Finally, the data followed first order kinetics for analysed mycotoxins and thermal constant rates (k) were calculated. This parameter can be a useful tool for prediction of mycotoxin levels.

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“…Furthermore, Fg16F/R primers are used for molecular identification of other fungi belonging to FGSC. In some studies, it was observed that they amplified products of different size, i.e., about 400 bp in F. graminearum , about 550 bp in F. asiaticum and about 500 bp in F. meridionale [Castañares et al, 2014;Covarelli et al, 2011]. Jurado et al [2005] developed species-specific primers for F. graminearum based on the IGS region.…”

Section: Pcr Assaysmentioning

confidence: 99%

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat

Kuzdraliński

Kot²,

Szczerba³

et al. 2017

Microb Physiol

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Infection of phyllosphere (stems, leaves, husks, and grains) by pathogenic fungi reduces the wheat yield and grain quality. Detection of the main wheat pathogenic fungi provides information about species composition and allows effective and targeted plant treatment. Since conventional procedures for the detection of these organisms are unreliable and time consuming, diagnostic DNA-based methods are required. Nucleic acid amplification technologies are independent of the morphological and biochemical characteristics of fungi. Microorganisms do not need to be cultured. Therefore, a number of PCR-based methodologies have been developed for the identification of key pathogenic fungi, such as Fusarium spp., Puccinia spp., Zymoseptoria tritici, Parastagonospora nodorum, Blumeria graminis f. sp. tritici, and Pyrenophora tritici-repentis. This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests for Fusarium graminearum, Puccinia spp., and P. tritici-repentis are insufficient.

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Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina

Cited by 57 publications

References 34 publications

Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay

Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay

Thermal stability and kinetics of degradation of deoxynivalenol, deoxynivalenol conjugates and ochratoxin A during baking of wheat bakery products

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat

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