The
assessment of food freshness is of paramount significance for
the maintenance of human health. However, the presence of an interfering
background signal from food samples often leads to inevitable false
negative results, which remains a formidable challenge in the rapid
assessment of food freshness. To address this issue, a bioinspired
anti-interfering triple-emission ratiometric fluorescent sensor was
developed based on a deep learning strategy to enhance the signal-to-noise
ratio in complex real sample and to allow for the rapid real-time
detection with significantly reduced sample size. It was enriched
with tubular foot-like functional groups (–NH2 and
–COOH), which showed good linearity between pH 2.5–9.5
with successive fluorescence color change from blue-green to light
green, light yellow, orange, and red. Three YOLO deep learning algorithm
models were used to construct self-designed smart WeChat applets for
high-throughput analysis, and two unique 3D printing toolboxes based
on a 96-well plate and cuvette for sample analysis were also designed.
The rapid high-throughput classification of a wide range of beverages
and real-time monitoring of food freshness based on a hydrogel tag
were also validated for reference. Prospectively, deep learning-assisted
creation of proportional sensors will be critical to increasing the
diversity and high throughput of real-time monitoring.