Triclocarban shifted the microbial communities and promoted the spread of antibiotic resistance genes in nitrifying granular sludge system

Wang, Zhiqi; Gao, Jingfeng; Wang, Shijie; Zhao, Yifan; Dai, Huihui; Li, Dingchang; Cui, Yingchao; Li, Ziqiao

doi:10.1016/j.biortech.2021.126429

Cited by 25 publications

(11 citation statements)

References 41 publications

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“…Wang et al reported that multidrug, sulfonamide, aminoglycoside, and tetracycline resistance genes remarkably increased after TCC addition. 30 However, TCC addition was found to have no significant relation with ARG proliferation in this study (P > 0.05), possibly because the background concentration of TCC in the sludge was sufficient to induce an effect. 16 Based on a co-occurrence network analysis among the top 100 microorganisms and top 60 ARGs, limited positive correlations were assigned to ARGs that were enriched on anode biofilms (Figure 4b).…”

Section: Changes In Metaboliccontrasting

confidence: 62%

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Mitigation of Triclocarban Inhibition in Microbial Electrolysis Cell-Assisted Anaerobic Digestion

Long,

Liu,

Xiao

et al. 2024

Environ. Sci. Technol.

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Triclocarban (TCC), as a widely used antimicrobial agent, is accumulated in waste activated sludge at a high level and inhibits the subsequent anaerobic digestion of sludge. This study, for the first time, investigated the effectiveness of microbial electrolysis cell-assisted anaerobic digestion (MEC-AD) in mitigating the inhibition of TCC to methane production. Experimental results showed that 20 mg/L TCC inhibited sludge disintegration, hydrolysis, acidogenesis, and methanogenesis processes and finally reduced methane production from traditional sludge anaerobic digestion by 19.1%. Molecular docking revealed the potential inactivation of binding of TCC to key enzymes in these processes. However, MEC-AD with 0.6 and 0.8 V external voltages achieved much higher methane production and controlled the TCC inhibition to less than 5.8%. TCC in the MEC-AD systems was adsorbed by humic substances and degraded to dichlorocarbanilide, leading to a certain detoxification effect. Methanogenic activities were increased in MEC-AD systems, accompanied by complete VFA consumption. Moreover, the applied voltage promoted cell apoptosis and sludge disintegration to release biodegradable organics. Metagenomic analysis revealed that the applied voltage increased the resistance of electrode biofilms to TCC by enriching functional microorganisms (syntrophic VFA-oxidizing and electroactive bacteria and hydrogenotrophic methanogens), acidification and methanogenesis pathways, multidrug efflux pumps, and SOS response.

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Section: Changes In Metaboliccontrasting

confidence: 62%

“…29 correlations (P < 0.05, 0.8 < r ≤ 1.0 or −1.0 ≤ r < −0.8) were retained and visualized through a co-occurrence network by Gephi 0.9.3. 30 A t test was performed to check the significance of data differences by SPSS Statistics 19 software.…”

Section: Substrate and Inoculum Was In A Secondarymentioning

confidence: 99%

Mitigation of Triclocarban Inhibition in Microbial Electrolysis Cell-Assisted Anaerobic Digestion

Long,

Liu,

Xiao

et al. 2024

Environ. Sci. Technol.

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“…The presence of triclosan in sewage sludge makes it an important source of antibiotic resistance genes, and mutations in the enoyl-acyl carrier protein (ACP) reductase gene have been discovered in sensitive opportunistic bacteria exposed to triclosan [ 48 ]. Triclocarban exposure in sewage sludge can reduce antibiotic tolerance and promote the selection of cross-resistant and multidrug-resistant genes, helping ARGs and MGEs (antibiotic resistance genes and mobile genetic elements) propagate in bacterial communities [ 49 , 50 ].…”

Section: Parabens Triclocarban and Triclosan: The Dark Side Of Person...mentioning

confidence: 99%

Personal Care Products as a Contributing Factor to Antimicrobial Resistance: Current State and Novel Approach to Investigation

et al. 2023

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Antimicrobial resistance (AMR) is one of the world’s industrialized nations’ biggest issues. It has a significant influence on the ecosystem and negatively affects human health. The overuse of antibiotics in the healthcare and agri-food industries has historically been defined as a leading factor, although the use of antimicrobial-containing personal care products plays a significant role in the spread of AMR. Lotions, creams, shampoos, soaps, shower gels, toothpaste, fragrances, and other items are used for everyday grooming and hygiene. However, in addition to the primary ingredients, additives are included to help preserve the product by lowering its microbial load and provide disinfection properties. These same substances are released into the environment, escaping traditional wastewater treatment methods and remaining in ecosystems where they contact microbial communities and promote the spread of resistance. The study of antimicrobial compounds, which are often solely researched from a toxicological point of view, must be resumed considering the recent discoveries, to highlight their contribution to AMR. Parabens, triclocarban, and triclosan are among the most worrying chemicals. To investigate this issue, more effective models must be chosen. Among them, zebrafish is a crucial study system because it allows for the assessment of both the risks associated with exposure to these substances as well as environmental monitoring. Furthermore, artificial intelligence-based computer systems are useful in simplifying the handling of antibiotic resistance data and speeding up drug discovery processes.

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“…Triclocarban (3,4,4′-trichlorocarbanilide, TCC), a broadspectrum antibacterial agent, has been widely used in personal care products (PCPs) such as detergents, cosmetics, and bath lotions. 1 Especially due to the epidemic of COVID-19, the dramatically increasing use of these TCC-containing products led to the striking accumulation of TCC in the environment. As a result, the concentration of TCC was found to range from 6.0 to 338 ng/L on the surface of water 2 and up to 43 mg/kg in sludge.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Triclocarban Exposure Induced Enterotoxicity by Triggering Intestinal AhR-Mediated Inflammation and Disrupting Microbial Community in Mice

Song,

Lei,

Cao

et al. 2024

Chem. Res. Toxicol.

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Exposure to triclocarban (TCC), a commonly used antibacterial agent, has been shown to induce significant intestine injuries and colonic inflammation in mice. However, the detailed mechanisms by which TCC exposure triggered enterotoxicity remain largely unclear. Herein, intestinal toxicity effects of longterm and chronic TCC exposure were investigated using a combination of histopathological assessments, metagenomics, targeted metabolomics, and biological assays. Mechanically, TCC exposure caused induction of intestinal aryl hydrocarbon receptor (AhR) and its transcriptional target cytochrome P4501A1 (Cyp1a1) leading to dysfunction of the gut barrier and disruption of the gut microbial community. A large number of lipopolysaccharides (LPS) are released from the gut lumen into blood circulation owing to the markedly increased permeability and gut leakage. Consequently, toll-like receptor-4 (TLR4) and NF-κB signaling pathways were activated by high levels of LPS. Simultaneously, classic macrophage phenotypes were switched by TCC, shown with marked upregulation of macrophage M1 and downregulation of macrophage M2 that was accompanied by striking upregulation of proinflammatory factors such as Il-1β, Il-6, Il-17, and Tnf-α in the intestinal lamina propria. These findings provide new evidence for the TCC-induced enterotoxicity.

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Triclocarban shifted the microbial communities and promoted the spread of antibiotic resistance genes in nitrifying granular sludge system

Cited by 25 publications

References 41 publications

Mitigation of Triclocarban Inhibition in Microbial Electrolysis Cell-Assisted Anaerobic Digestion

Mitigation of Triclocarban Inhibition in Microbial Electrolysis Cell-Assisted Anaerobic Digestion

Personal Care Products as a Contributing Factor to Antimicrobial Resistance: Current State and Novel Approach to Investigation

Long-Term Triclocarban Exposure Induced Enterotoxicity by Triggering Intestinal AhR-Mediated Inflammation and Disrupting Microbial Community in Mice

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