Trigalactosyldiacylglycerol 3 protein orthologs are required for basal disease resistance in &lt;i&gt;Nicotiana benthamiana&lt;/i&gt;

Tagami, Shuhei; Ohnishi, Kouhei; Hikichi, Yasufumi; Kiba, Akinori

doi:10.5511/plantbiotechnology.21.0624a

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Furthermore, we detected putative serine/threonine-protein kinases EDR1, g19355 (log 2 FC+2.6) and g55470 (log 2 FC+2.6), homologs of two proteins which in A. thaliana are involved in crosstalk between ethylene-, ABA- and salicylic acid signaling (Tang et al, 2005; Wawrzynska et al, 2008). Additionally, we detected with a log 2 FC of +4.1 high differential expression of g23946, encoding a homolog of the trigalactosyldiacylglycerol 3 (TGD3) protein, involved in chloroplast lipid import (Lu et al, 2007), jasmonic acid signalling and plant defence responses via phosphatidic acid signalling (Tagami et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Insight into the central and nodal cells transcriptome of the streptophyte green algaChara brauniiS276

Heß

Holzhausen

Hess

2023

Preprint

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Chara braunii is an emerging model system for streptophyte terrestrialization and early land plant evolution. In this study, tissue containing nodal cells was prepared under the stereomicroscope and an RNA-seq dataset generated and compared to transcriptome data from whole plantlets. In both samples, transcript coverage was high for genes encoding ribosomal proteins and a homolog of the putative PAX3- and PAX7-binding protein 1. Gene ontology-based functional assignments revealed for the nodal cell sample main upregulated molecular functions related to protein binding, nucleic acid binding and DNA binding. Looking at specific genes, several signalling-related genes and genes encoding sugar-metabolizing enzymes were found to be expressed at a higher level in the nodal cell sample, while photosynthesis-and chloroplast-related genes were expressed at a comparatively lower level. We detected the transcription of 21 different genes encoding DUF4360-containing cysteine-rich proteins. The data contribute to the growing understanding of charophyte developmental genetics by providing a first insight into the transcriptome composition of Chara central and nodal cells.

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Section: Resultsmentioning

confidence: 99%

Insight into the central and nodal cells transcriptome of the streptophyte green algaChara brauniiS276

Heß

Holzhausen

Hess

2023

Preprint

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show abstract

“…Furthermore, we detected putative serine/threonine‐protein kinases EDR1, g19355 (log 2 FC +2.6) and g55470 (log 2 FC +2.59), homologs of two proteins involved in crosstalk between ethylene‐, ABA‐ and salicylic acid signaling in A. thaliana (Tang et al, 2005; Wawrzynska et al, 2008). Additionally, we detected with a log 2 FC of +4.1 enrichment of g23946, encoding a homolog of the trigalactosyldiacylglycerol 3 (TGD3) protein, involved in chloroplast lipid import (Lu et al, 2007), jasmonic acid signaling and plant defense responses via phosphatidic acid signaling (Tagami et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, we detected with a log 2 FC of +4.1 enrichment of g23946, encoding a homolog of the trigalactosyldiacylglycerol 3 (TGD3) protein, involved in chloroplast lipid import (Lu et al, 2007), jasmonic acid signaling and plant defense responses via phosphatidic acid signaling (Tagami et al, 2021).…”

Section: Functional Assignments To Differentially Expressed Genesmentioning

confidence: 99%

Insight into the nodal cells transcriptome of the streptophyte green alga Chara braunii S276

Heß,

Holzhausen,

Hess

2023

Physiologia Plantarum

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Charophyceae are the most complex streptophyte algae, possessing tissue‐like structures, rhizoids and a cellulose‐pectin‐based cell wall akin to embryophytes. Together with the Zygnematophyceae and the Coleochaetophycae, the Charophyceae form a grade in which the Zygnematophyceae share a last common ancestor with land plants. The availability of genomic data, its short life cycle, and the ease of non‐sterile cultivation in the laboratory have made the species Chara braunii an emerging model system for streptophyte terrestrialization and early land plant evolution. In this study, tissue containing nodal cells was prepared under the stereomicroscope, and an RNA‐seq dataset was generated and compared to transcriptome data from whole plantlets. In both samples, transcript coverage was high for genes encoding ribosomal proteins and a homolog of the putative PAX3‐ and PAX7‐binding protein 1. Gene ontology was used to classify the putative functions of the differently expressed genes. In the nodal cell sample, main upregulated molecular functions were related to protein, nucleic acid, ATP‐ and DNA binding. Looking at specific genes, several signaling‐related genes and genes encoding sugar‐metabolizing enzymes were found to be expressed at a higher level in the nodal cell sample, while photosynthesis‐and chloroplast‐related genes were expressed at a comparatively lower level. We detected the transcription of 21 different genes encoding DUF4360‐containing cysteine‐rich proteins. The data contribute to the growing understanding of Charophyceae developmental biology by providing a first insight into the transcriptome composition of Chara nodal cells.

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A ScWIP5 gene confers fall armyworm resistance by reducing digestive enzyme activities in sugarcane

Wang,

Yao,

Chen

et al. 2024

Pest Management Science

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BackgroundThe fall armyworm, Spodoptera frugiperda, is one of the most dangerous pests to various crops. As the most crucial sugar crop, sugarcane is also constantly threatened by these pests. Plant wound‐induced proteinase inhibitors (WIP) are natural defense proteins that play important roles in the defense system against insect attack. Breeding for resistance would be the best way to improve the variety characteristics and productivity of sugarcane. Screening and verification for potential plant endogenous insect‐resistant genes would greatly improve the insect‐resistant breeding progress of sugarcaneResultsA sugarcane WIP5 gene (ScWIP5) was 536 times up regulated after insect feeding treatment on previous published transcriptome databases. Then, ScWIP5 was cloned and evaluated its potential role in sugarcane resistance to fall armyworm by construction of transgenic Nicotiana benthamiana. The toxicity of ScWIP5 transgenic N. benthamiana to fall armyworm showed lower weight gain and higher mortality compared to wild‐type N. benthamiana feeding group. Furthermore, the concentration of jasmonic acid (JA) and NbAOC, NbAOS, NbLOX from Jasmin acid biosynthesis pathway was significantly induced in ScWIP5 transgenic N. benthamiana compared to control. In addition, digestive enzyme actives from the insect gut were also evaluated, and trypsin and cathepsin were significantly lower in insects fed with ScWIP5 transgenic N. benthamiana.ConclusionThese results indicated that ScWIP5 might enhance insect‐resistance by increasing JA signal transduction processes and reducing insect digestive enzyme activities, thus impacting insect growth and development.This article is protected by copyright. All rights reserved.

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Trigalactosyldiacylglycerol 3 protein orthologs are required for basal disease resistance in <i>Nicotiana benthamiana</i>

Cited by 4 publications

References 32 publications

Insight into the central and nodal cells transcriptome of the streptophyte green algaChara brauniiS276

Insight into the central and nodal cells transcriptome of the streptophyte green algaChara brauniiS276

Insight into the nodal cells transcriptome of the streptophyte green alga Chara braunii S276

A ScWIP5 gene confers fall armyworm resistance by reducing digestive enzyme activities in sugarcane

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