Triggered insulin release studies of triply responsive supramolecular micelles

Abstract

The synthesis of a supramolecular double hydrophilic glucose responsive block copolymer (DHBC) held together by cucurbit [8]uril (CB[8]) ternary complexation and its subsequent self-assembly into micelles is described. The supramolecular block copolymer assembly consists of poly(Nisopropylacrylamide) (PNIPAAm) and poly(acrylamidophenyl boronic acid) (PAAPBA) as temperature and glucose responsive blocks, respectively, and poly(dimethylacrylamide) (PDMAAm) as a hydrophilic block. Drug release studies of insulin-loaded micelles using three external triggers were studied with release of insulin achieved by changing temperature, glucose concentration or by adding a competitive guest for CB[8]. This system offers good control over the release of insulin under physiological conditions (pH 7.4, 37 C). These exciting results suggest that this system could be a model for a clinically relevant drug delivery vehicle for diabetic treatment. Fig. 1 Formation of the boronic acid-glucose complex.

Multi-Stimuli-Responsive Polymer Materials: Particles, Films, and Bulk Gels

“…It was found that the hydrophilic PDMAAm segments could be removed from the micelles in the presence of the adamantaneamine, resulting in rupture of the micellar superstructures and enabling complete release of insulin from the hierarchical micelles. The most important feature was that this system displayed a pulsated release of insulin with the three different triggers of temperature, glucose, and a competitive guest, and would have potential for insulin therapies and the treatment of diabetes in “on‐demand'' release 144…”

Latest Advances in Antibacterial Materials

“…The macrocyclic host, cucurbit [7]uril (CB [7]) was chosen as the model. Cucurbit[n]urils (CB[n]s) are a family of water-soluble macrocyclic hosts, which have a hydrophobic cavity capable of the binding of one or two guest molecules depending on the size of di®erent CB[n]s. [22][23][24] CB [7] possesses good solubility in water and strong a±nity for one guest molecule. [25][26][27][28][29][30][31] The authors employed CB [7] to bind with the positive charge and hydrophobic component on "-poly-l-lysine hydrochlorides to construct the polypseudorotaxane for antibacterial regulation.…”

Engineering Bioresponsive Hydrogels toward Healthcare Applications

“…Besides CD‐based supramolecular systems, cucurbit[8]uril (CB[8]) ternary complexation with bioresponsive multiple‐block copolymers has also attracted many interests recently. We reported that block copolymer, containing temperature sensitive PNIPAAm block, glucose responsive poly(acrylamidophenyl boronic acid) (PAAPBA) block, and poly(dimethylacrylamide) (PDMAAm) as hydrophobic block could act as insulin carrier and release insulin drug upon triggers of temperature, high glucose concentration, and addition of CB[8] as competitive guest, indicating its potential drug delivery application for diabetic treatment. Further investigation revealed that a copolymer consisting of special end‐functionalised PNIPAAm and pH sensitive PDMAEMA could self‐assemble with CB[8] to form bioresponsive supramolecules, with the ability to triply trigger the release of encapsulated doxorubicin and effectively induce HeLa cell viability reduction in terms of temperature, pH, and competitive CB[8] amount .…”