Triggering of high-speed neurite outgrowth using an optical microheater

Oyama, Kotaro; Zeeb, Vadim; Kawamura, Yuki I.; Arai, Tomomi; Gotoh, Mizuho; Itoh, Hideki; Itabashi, Takeshi; Suzuki, Madoka; Ishiwata, Shin’ichi

doi:10.1038/srep16611

Cited by 37 publications

(38 citation statements)

References 54 publications

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“…Oyama et al . demonstrate that the rapid increase of local temperature from 36°C to 41°C in rat hippocampal neurons results in elongation of neurites by more than 10 μ m within 1 min . Therefore, it is possible that axonal outgrowth of IAN from the transected portion to the mechanically stimulated site in the facial skin above the mental foramen is induced within a day or so by LIPUS.…”

Section: Discussionmentioning

confidence: 96%

“…Although the distance between the transected portion and the mechanically stimulated site in the facial skin above the mental foramen was about 15 mm, the sensory loss in facial skin above the mental foramen following IANX was recovered on day 1 after LIPUS of the transected IAN. OYAMA et al demonstrate that the rapid increase of local temperature from 36°C to 41°C in rat hippocampal neurons results in elongation of neurites by more than 10 lm within 1 min (32). Therefore, it is possible that axonal outgrowth of IAN from the transected portion to the mechanically stimulated site in the facial skin above the mental foramen is induced within a day or so by LIPUS.…”

Section: Discussionmentioning

confidence: 99%

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Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Sato

Motoyoshi

Shinoda

et al. 2016

European J Oral Sciences

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Inferior alveolar nerve (IAN) injury, which is frequently caused by orofacial surgery or trauma, induces sensory loss in orofacial regions innervated by the IAN. However, no effective treatment for orofacial sensory loss currently exists. We determined whether sensory loss in facial skin above the mental foramen following IAN transection was recovered by exposure of the transected IAN to low-intensity pulsed ultrasound (LIPUS). Inferior alveolar nerve transection (IANX) was performed in 7-wk-old male Sprague-Dawley rats. On day 7 after IANX, the effect of daily LIPUS (from day 0) on the transected IAN, in terms of sensitivity to mechanical stimulation of the facial skin above the mental foramen, was examined. Moreover, the number of trigeminal ganglion (TG) neurons innervating the facial skin above the mental foramen of rats with IANX treated daily with LIPUS was counted using the retrograde neurotracing technique. Daily exposure of the transected IAN to LIPUS significantly promoted recovery of the head-withdrawal threshold in response to mechanical stimulation of the facial skin above the mental foramen, and the number of TG neurons innervating the facial skin above mental foramen was significantly increased in rats with IANX treated daily with LIPUS compared with sham or LIPUS-unexposed rats. Daily treatment of stumps of the transected IAN with LIPUS facilitated morphological and functional regeneration, suggesting that LIPUS is an effective and novel therapy for IAN injury.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Sato

Motoyoshi

Shinoda

et al. 2016

European J Oral Sciences

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“…Ca 2+ -dynamics and thus different Ca 2+ channels are known to regulate the neuronal growth cone dynamics 12 . Rapid neurite outgrowth can also be triggered by laser-based micro-heating, suggesting that neurites have unique ability to sense minor temperature differences also 13 . Similarly, mechanosensitive Ca 2+ -channels have also been implicated in the initiation of neuritogenesis 14 .…”

Section: Introductionmentioning

confidence: 99%

TRPV2 activation reorganizes actin cytoskeleton, induces neurite initiation and branching by altering cAMP levels

Yadav

Goswami

2019

Preprint

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Understanding of molecules and their role in neurite initiation and/or extension is not only helpful to prevent different neurodegenerative diseases but also can be important by which neuronal damages can be repaired. In this work we explored the role of TRPV2, a non-selective cation channel in the context of neurite functions. Using western blot, immunofluorescence, and live cell Ca 2+ -imaging;we confirm that functional TRPV2 is endogenously present in the F11 cell, a model system mimicking peripheral neuron. In F11 cells TRPV2 localizes in specific sub-cellular regions enriched with filamentous actin, such as in growth cone, filopodia, lamellipodia and in neurites. TRPV2 regulates actin cytoskeleton and also interacts with soluble actin. Ectopic expression of TRPV2-GFP but not GFP-only in F11 cell induces more primary and secondary neurites, confirming its role in neurite initiation, extension and branching events. TRPV2-mediated neuritogenesis is dependent on wild-type TRPV2 as cells expressing TRPV2 mutants reveal no neuritogenesis. However, TRPV2-mediated neuritogenesis is unperturbed by the chelation of intracellular Ca 2+ by BAPTA-AM, and thus involves Ca 2+ -independent signaling events also. We demonstrate that pharmacological modulation of TRPV2 alters cellular cAMP levels. These findings are relevant to understand the sprouting of new neurites, neuroregeneration and neuronal plasticity at the cellular, subcellular and molecular level. Such understanding may have border implication in neurodegeneration and peripheral neuropathy.

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“…This way, a global response is observed potentially masking a possible influence of local temperature effects. Heating cells in only a localized area can be achieved using a focused infrared laser . This approach has been demonstrated for the excitation of action potentials in muscle and nerve cells .…”

Section: Introductionmentioning

confidence: 99%

Chip‐Based Heat Stimulation for Modulating Signal Propagation in HL‐1 Cell Networks

et al. 2018

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Signal propagation in cardiac cell networks can be modulated by heat stimulation. Here, the response of a connected HL‐1 cardiomyocyte cell network to the application of confined heat stimuli using Ca2+ imaging is investigated. Localized temperature gradients are generated by resistive heating via microwire arrays on a chip surface, which serves as a substrate for growing a confluent cell network. It is demonstrated that upon heat stimulation, the velocity of the propagating Ca2+ wave in the network is locally increased, leading to a deformation of the wavefront. Furthermore, evidence of a change in the signal propagation direction caused by a relocation of the pacemaker cell is shown. This effect might be used in future applications, where heat is employed as an alternative modality for cell stimulation protocols.

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Triggering of high-speed neurite outgrowth using an optical microheater

Cited by 37 publications

References 54 publications

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

TRPV2 activation reorganizes actin cytoskeleton, induces neurite initiation and branching by altering cAMP levels

Chip‐Based Heat Stimulation for Modulating Signal Propagation in HL‐1 Cell Networks

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