Triggering of the Antibacterial Activity of Bacillus subtilis B38 Strain against Methicillin-Resistant Staphylococcus aureus

Tabbene, Olfa; Karkouch, Ines; Slimene, Imen Ben; Elfeddy, Najib; Cosette, Pascal; Mangoni, Maria Luisa; Jouenne, Thierry; Limam, Férid

doi:10.1007/s12010-010-9112-z

Cited by 4 publications

(4 citation statements)

References 34 publications

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“…These results indicated that, in the presence of the pathogen (MRSA ATCC 33742), strain PD9 produced more antimicrobial metabolites. The induction of antimicrobial metabolite in the presence of competing species or pathogens was also observed in related studies of B. subtilis B38 against MRSA [47] and soil bacterial isolates against E. coli and S. aureus [48]. From the MBC analysis, it was found that the CFS had bactericidal activity.…”

Section: Plos Onementioning

confidence: 58%

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Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

et al. 2021

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Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have reached epidemic proportions globally. Therefore, there is an urgent need for a continuous supply of antibiotics to combat the problem. In this study, bacteria initially identified as species belonging to the Bacillus amyloliquefaciens operational group were re-identified based on the housekeeping gene, gyrB. Cell-free supernatants (CFS) from the strains were used for antimicrobial tests using the agar well diffusion assay against MRSA and various types of pathogenic bacteria. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and physicochemical characteristics of the CFS were determined. Based on gyrB sequence analysis, five strains (PD9, B7, PU1, BP1 and L9) were identified as Bacillus velezensis. The CFS of all B. velezensis strains showed broad inhibitory activities against Gram-negative and -positive as well as MRSA strains. Strain PD9 against MRSA ATCC 33742 was chosen for further analysis as it showed the biggest zone of inhibition (21.0 ± 0.4 mm). The MIC and MBC values obtained were 125 μl/ml. The crude antimicrobial extract showed bactericidal activity and was stable at various temperatures (40–80°C), pH (4–12), surfactants (Tween 20, Tween 80, SDS and Triton X-100) and metal ions (MgCI2, NaCI2, ZnNO3 and CuSO4) when tested. However, the crude extract was not stable when treated with proteinase K. All these properties resembled the characteristics of peptides. The antimicrobial compound from the selected strain was purified by using solvent extraction method and silica gel column chromatography. The purified compound was subjected to High Performance Liquid Chromatography which resulted in a single peak of the anti-MRSA compound being detected. The molecular weight of the anti-MRSA compound was determined by using SDS-PAGE and zymogram. The size of the purified antimicrobial peptide was approximately ~ 5 kDa. The antimicrobial peptide produced from B. velezensis strain PD9 is a promising alternative to combat the spread of MRSA infections in the future.

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Section: Plos Onementioning

confidence: 58%

“…The induction of antimicrobial metabolite in the presence of competing species or pathogens was also observed in related studies of B . subtilis B38 against MRSA [ 47 ] and soil bacterial isolates against E . coli and S .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

et al. 2021

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“…It has also been shown that individual marine biofilm isolates can be induced to express increased activity against S. aureus when grown in the presence of spent culture media from S. aureus, or in co-culture with S. aureus (Burgess et al 1999;Kanagasabhapathy and Nagata 2008). Similarly, Bacillus subtillus has been reported to have inducible activity against S. aureus (Tabbene et al 2011), and marine epibiotic isolates to have inducible activity against Pseudomonas aeruginosa (Dusane et al 2011). These studies featured selected bacteria which were known antibiotic producers.…”

Section: Discussionmentioning

confidence: 99%

Induction of resistance to S. aureus in an environmental marine biofilm grown in Sydney Harbor, NSW, Australia

Lafleur

Rice

2014

World J Microbiol Biotechnol

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The study of environmental biofilms is complicated by the difficulty of working with them under lab conditions. Nonetheless, knowledge of cellular activity and interactions within environmental biofilms could lead to novel biomedical applications. To address this problem we previously proposed a new technique for inducing resistance to Staphylococcus aureus in an intact environmental biofilm. In the current follow-up study we applied the new technique in a biogeographically distinct environment using a different strain of S. aureus. The proposed technique for inducing resistance to S. aureus in an environmental biofilm involves growing the environmental biofilms over several days in media reflecting their natural habitat on agar that contains spent culture supernatant from S. aureus over-night culture. We found in this second study that it was possible to induce resistance to S. aureus in an environmental biofilm from a biogeographically distinct environment, though not in the same way as we had previously observed. Environmental consortia from Sydney Harbor, Australia display an ability to inhibit biofilm formation by S. aureus; only in the case where the environmental biofilms were pretreated with UV radiation was there a difference in activity between environmental consortia grown on plain agar, and that grown on S. aureus agar. Application of the new technique in the current study also differs in that significant killing of cells within an established S. aureus biofilm by environmental consortia grown on S. aureus agar was possible.

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“…In contrast, the addition of Klebsiella weissii Br26 to fermentation supernatants at different growth stages resulted in a signi cant but not identical enhancement of its inhibitory activity, suggesting that the biosynthesis of bacteriocin can be self-induced in addition to receiving the in uence of the time of addition [50]. Similarly, the addition of further cell-free supernatant of Bacillus subtilis B38 increased its own antimicrobial activity by 8-fold to 160 AU/mL [51]. Summarizing the results of the above studies, it could be inferred that when the concentration of the inducing substance in the inducing supernatant reaches a certain threshold, the inducing effect appears; while when the inducing supernatant is added at the wrong time, the inducing effect does not occur.…”

Section: The Change Of Exo-metabolome On Plantaricin In Co-culturementioning

confidence: 99%

Bacteriocin-induced mechanism of Wickerhamomyces anomalus Y-5 co-cultured with Lactiplantibacillus paraplantarum RX-8 by transcriptomic and proteomic analysis

Liu,

Nie,

Sun

et al. 2024

Preprint

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Bacteriocin is a broad-spectrum antimicrobial active metabolite with a high potential for application in the food field. The previous studies found that co-culture of Wickerhamomyces anomalus Y-5 and Lactiplantibacillus paraplantarum RX-8 could increase the production of plantaricin RX-8. In order to investigate the induced mechanism of W. anomalus Y-5 in co-culture, this study explored the effects of induction components and contact mode on plantaricin RX-8; followed by transcriptomic and proteomic analyses of W. anomalus Y-5 in mono and co-culture systems, and screened differential metabolites by targeted metabolomic; finally, the potential inducing substances were subjected to validation experiments. The results indicated that the induced effect may not require direct cell contact, rather secretions constant stimulation. In co-culture system, W. anomalus Y-5 reduced nitrogen uptake, which allowed the release of the active Tap42 protein into the cytoplasm to stimulate the expression of retrograde genes, maintained biosynthesis of glutamic acid and glutamine. In addition, W. anomalus Y-5 was subjected to acid and osmotic stress, which resulted in activation of cAMP synthesis, inhibition of Ras protein activity, and up-regulation of Hxk2 expression. Further, we found that glutamine, inosine, guanosine, adenine, uracil, fumaric acid and pyruvic acid were the key substances that induced the production of plantaricin RX-8, and the optimal addition time was 8 h. In conclusion, these findings provided new perspectives on the identification of inducing substances and the analysis of production pathways in the efficient synthesis of bacteriocin induced by fungi, and lay the foundation for the industrial production of bacteriocin.

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Triggering of the Antibacterial Activity of Bacillus subtilis B38 Strain against Methicillin-Resistant Staphylococcus aureus

Cited by 4 publications

References 34 publications

Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

Induction of resistance to S. aureus in an environmental marine biofilm grown in Sydney Harbor, NSW, Australia

Bacteriocin-induced mechanism of Wickerhamomyces anomalus Y-5 co-cultured with Lactiplantibacillus paraplantarum RX-8 by transcriptomic and proteomic analysis

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