Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs

Xu, Huo; Yang, Fenglin; Chen, Danlong; Ye, Weilin; Xue, Guohui; Lee, Jia

doi:10.1016/j.isci.2023.106331

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Hosseinzadeh et al reported a triple amplification strategy to detect miR-21, in which miR-21 triggered the formation of G-quadruplex DNAzymes to give a colorimetric signal [95]. Recent distinct strategies for G-quadruplex DNAzyme production include (1) target miRNA-triggered RCA to produce numerous G-quadruplex DNAzymes (Figure 5D) [96,97]; (2) miRNA-triggered RCA coupled with nicking enzyme assistance to produce G-quadruplex DNAzymes [98,99], (3) miRNA-induced toehold-mediated strand displacement reactions to release G-quadruplexes (Figure 5B) [100][101][102][103][104][105]; (4) nicking enzyme-assisted strand displacement amplification (Figure 5C) [106][107][108]; (5) target miRNA-triggered HCR to form long G-quadruplex DNAzyme chains [109]; and (6) DSNassisted G-quadruplex DNAzyme release [110,111]. Beyond these, there are also some special colorimetric assay strategies based on G-quadruplexes.…”

Section: Colorimetric Methods Based On Enzymatic Chromogenic Reactionsmentioning

confidence: 99%

Advances in Point-of-Care Testing of microRNAs Based on Portable Instruments and Visual Detection

et al. 2023

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MicroRNAs (miRNAs) are a class of small noncoding RNAs that are approximately 22 nt in length and regulate gene expression post-transcriptionally. miRNAs play a vital role in both physiological and pathological processes and are regarded as promising biomarkers for cancer, cardiovascular diseases, neurodegenerative diseases, and so on. Accurate detection of miRNA expression level in clinical samples is important for miRNA-guided diagnostics. However, the common miRNA detection approaches like RNA sequencing, qRT-PCR, and miRNA microarray are performed in a professional laboratory with complex intermediate steps and are time-consuming and costly, challenging the miRNA-guided diagnostics. Hence, sensitive, highly specific, rapid, and easy-to-use detection of miRNAs is crucial for clinical diagnosis based on miRNAs. With the advantages of being specific, sensitive, efficient, cost-saving, and easy to operate, point-of-care testing (POCT) has been widely used in the detection of miRNAs. For the first time, we mainly focus on summarizing the research progress in POCT of miRNAs based on portable instruments and visual readout methods. As widely available pocket-size portable instruments and visual detection play important roles in POCT, we provide an all-sided discussion of the principles of these methods and their main limitations and challenges, in order to provide a guide for the development of more accurate, specific, and sensitive POCT methods for miRNA detection.

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Section: Colorimetric Methods Based On Enzymatic Chromogenic Reactionsmentioning

confidence: 99%

Advances in Point-of-Care Testing of microRNAs Based on Portable Instruments and Visual Detection

et al. 2023

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“…Compared to traditional exponential amplification techniques, cascade amplification offers the advantage of not only achieving higher signal intensity but also maintaining lower background noise. 78,79,92 By integrating SDA with EXPAR, this approach facilitates a significant enhancement in both detection specificity and sensitivity.…”

Section: Sdamentioning

confidence: 99%

EXPAR for biosensing: recent developments and applications

Ou,

Li,

Liu

et al. 2024

Analyst

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“…25 Recently, the SDA-mediated G4 fluorescence method has been applied to the detection of biological targets. 26,27 For example, Moon et al reported a novel 3WJ-triggered SDA and G4 recycling fluorescence detection method for sensitive detection of exosomal mRNA in urine samples. 28 Consequently, it is an ideal strategy that combines the SDA and G4 fluorescence detection to achieve a higher detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Cascade branch migration-triggered strand displacement amplification for specific and sensitive detection of microRNA

Xie,

Hou,

et al. 2024

Anal. Methods

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Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs

Cited by 3 publications

References 24 publications

Advances in Point-of-Care Testing of microRNAs Based on Portable Instruments and Visual Detection

Advances in Point-of-Care Testing of microRNAs Based on Portable Instruments and Visual Detection

EXPAR for biosensing: recent developments and applications

Cascade branch migration-triggered strand displacement amplification for specific and sensitive detection of microRNA

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