Trigonelline, a plant derived alkaloid prevents ultraviolet-B-induced oxidative DNA damage in primary human dermal fibroblasts and BALB/c mice via modulation of phosphoinositide 3-kinase-Akt-Nrf2 signalling axis

Tanveer, Malik A.; Rashid, Haroon; Nazir, Lone A.; Archoo, Sajida; Shahid, Naiku H; Gupta, Ragni; Ahmad, Sheikh Umar; Tasduq, Sheikh A.

doi:10.1016/j.exger.2022.112028

Cited by 21 publications

(19 citation statements)

References 34 publications

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“…Certainly, ongoing research is dedicated to identifying compounds capable of mitigating skin photoaging, particularly emphasizing natural substances and their derivatives renowned for their antioxidant and anti‐inflammatory attributes. These include resveratrol‐enriched rice, 7 probiotics, 56 flavonoids, carotenoids, 57 ginsenoside, 58 glycyrrhizic acid, 59 and trigonalline, 60 among others.…”

Section: Discussionmentioning

confidence: 99%

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

Peng,

Li,

Zeng

et al. 2024

J of Cosmetic Dermatology

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BackgroundResearch has demonstrated the anti‐photoaging properties of glabridin and bakuchiol.MethodsThe impact of glabridin, glabridin + bakuchiol, and bakuchiol on the levels of tumor necrosis factor alpha (TNF‐α) and interleukin‐1 beta (IL‐1β) in mice skin fibroblasts was observed. Furthermore, we investigated the potential roles of fibronectin (FN), interferon‐γ (IFN‐γ), interleukin‐22 (IL‐22), and transforming growth factor‐β (TGF‐β) in the tissues, and evaluated their impact on the enzymatic levels in the skin. In conjunction with transcriptomic analysis, metabolomic profiling, and network pharmacology, all samples underwent comprehensive metabolomic and principal component analysis. The Venny2.1 method was utilized to identify variances in shared metabolites between the treatment group and the UVB group, as well as between the UVB group and the control group. Subsequently, a cluster heat map was generated to forecast and analyze metabolic pathways and targets.ResultsThe outcomes from the hematoxylin and eosin and toluidine blue staining revealed that glabridin and bakuchiol markedly decreased dermal thickness and suppressed mast cell infiltration in photoaged mice. Immunohistochemistry and Elisa analysis revealed that glabridin and bakuchiol effectively attenuated the levels of pro‐inflammatory factors, including IL‐1β, tumor necrosis factor‐α, IL‐22, and IFN‐γ. Furthermore, an increase in the levels of anti‐inflammatory factors such as FN and TGF‐β was also observed. The determination of the contents of superoxide dismutase, hydroxypropyltransferase and malondialdehyde in mice dorsal skin revealed that glabridin and bakuchiol not only elevated the levels of superoxide dismutase and hydroxyproline, but also reduced malondialdehyde content. Due to the limited number of shared differential metabolites exclusively within Kyoto Encyclopedia of Genes and Genomes, comprehensive pathway enrichment analysis was not feasible.ConclusionThis study demonstrates that glabridin and bakuchiol effectively impede photoaging and alleviate skin inflammation in mice.

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Section: Discussionmentioning

confidence: 99%

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

Peng,

Li,

Zeng

et al. 2024

J of Cosmetic Dermatology

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“…The five other metabolites, trigonelline, nicotinamide-N-oxide, shikimic acid, 5′-deoxy-5′-(methylthio)adenosine, and cis-aconitic acid were also poorly reported in Dendrobium for antioxidant activities. Trigonelline significantly alleviated UV-B-induced cell death effects in primary human dermal fibroblasts [ 31 ]. Shikimic acid from Artemisia absinthium enhanced antioxidant activity in diabetic rats [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Rao

Zhao

et al. 2023

Metabolites

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The medicinal plant Dendrobium nobile is an important natural antioxidant resource. To reveal the antioxidants of D. nobile, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for metabolic analysis. The H2O2-induced oxidative damage was used in human embryonic kidney 293T (H293T) cells to assess intracellular antioxidant activities. Cells incubated with flower and fruit extracts showed better cell survival, lower levels of reactive oxygen species (ROS), and higher catalase and superoxide dismutase activities than those incubated with root, stem, and leaf extracts (p < 0.01). A total of 13 compounds were newly identified as intracellular antioxidants by association analysis, including coniferin, galactinol, trehalose, beta-D-lactose, trigonelline, nicotinamide-N-oxide, shikimic acid, 5′-deoxy-5′-(methylthio)adenosine, salicylic acid, isorhamnetin-3-O-neohespeidoside, methylhesperidin, 4-hydroxybenzoic acid, and cis-aconitic acid (R2 > 0.8, Log2FC > 1, distribution > 0.1%, and p < 0.01). They showed lower molecular weight and higher polarity, compared to previously identified in vitro antioxidants in D. nobile (p < 0.01). The credibility of HPLC-MS/MS relative quantification was verified by common methods. In conclusion, some saccharides and phenols with low molecular weight and high polarity helped protect H293T cells from oxidative damage by increasing the activities of intracellular antioxidant enzymes and reducing intracellular ROS levels. The results enriched the database of safe and effective intracellular antioxidants in medicinal plants.

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“…Topical TRG protected mice skin from UV-B-induced apoptosis [ 45 ]. TRG prevented UV-B-induced oxidative stress by activating the PI3K/Akt/Nrf2 signaling pathway [ 46 ]. TRG prevented UV-B-induced collagen degradation and lipid peroxidation in Hs68 cells by attenuating MMP1 activity and prevented UV-B-induced cellular oxidative damage by modulating the ROS/MAPK/NF-κB axis [ 47 ].…”

Section: Other Protective Effects Of Trgmentioning

confidence: 99%

“…TRG shows various beneficial roles in many pathological conditions. It can (1) modulate glucose and lipid homeostasis [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ] ( A ); (2) suppress the inflammatory response and oxidative stress [ 11 , 12 , 13 , 14 ] ( B , H , M ); (2) facilitate recovery from neurological impairments such as neurodegenerative disorders [ 15 , 16 , 17 , 18 ], ischemia-induced brain damage [ 19 , 20 ], cognitive decline [ 21 , 22 , 23 ], diabetic peripheral neuropathy [ 24 , 25 ], depression, and epilepsy [ 26 , 27 , 28 ] ( C ); (3) mitigate DM and its complications [ 6 , 11 , 29 , 30 , 31 , 32 ] ( A – F ); (4) alleviate cellular injuries in the cardiovascular system [ 33 , 34 ], liver [ 35 , 36 ], kidney [ 37 , 38 , 39 , 40 , 41 , 42 ], gastric system [ 43 , 44 ], and skin [ 45 , 46 , 47 ] ( D – F , I – L ); and (5) inhibit proliferation and migration of tumor cells [ 48 , ...…”

Section: Figurementioning

confidence: 99%

Pharmacological Activities, Therapeutic Effects, and Mechanistic Actions of Trigonelline

Nguyen,

Taine,

Meng

et al. 2024

IJMS

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Trigonelline (TRG) is a natural polar hydrophilic alkaloid that is found in many plants such as green coffee beans and fenugreek seeds. TRG potentially acts on multiple molecular targets, including nuclear factor erythroid 2-related factor 2 (Nrf2), peroxisome proliferator-activated receptor γ, glycogen synthase kinase, tyrosinase, nerve growth factor, estrogen receptor, amyloid-β peptide, and several neurotransmitter receptors. In this review, we systematically summarize the pharmacological activities, medicinal properties, and mechanistic actions of TRG as a potential therapeutic agent. Mechanistically, TRG can facilitate the maintenance and restoration of the metabolic homeostasis of glucose and lipids. It can counteract inflammatory constituents at multiple levels by hampering pro-inflammatory factor release, alleviating inflammatory propagation, and attenuating tissue injury. It concurrently modulates oxidative stress by the blockage of the detrimental Nrf2 pathway when autophagy is impaired. Therefore, it exerts diverse therapeutic effects on a variety of pathological conditions associated with chronic metabolic diseases and age-related disorders. It shows multidimensional effects, including neuroprotection from neurodegenerative disorders and diabetic peripheral neuropathy, neuromodulation, mitigation of cardiovascular disorders, skin diseases, diabetic mellitus, liver and kidney injuries, and anti-pathogen and anti-tumor activities. Further validations are required to define its specific targeting molecules, dissect the underlying mechanistic networks, and corroborate its efficacy in clinical trials.

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Trigonelline, a plant derived alkaloid prevents ultraviolet-B-induced oxidative DNA damage in primary human dermal fibroblasts and BALB/c mice via modulation of phosphoinositide 3-kinase-Akt-Nrf2 signalling axis

Cited by 21 publications

References 34 publications

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

Effect of glabridin combined with bakuchiol on UVB‐induced skin damage and its underlying mechanism: An experimental study

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Pharmacological Activities, Therapeutic Effects, and Mechanistic Actions of Trigonelline

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