Triiodothyronine Modulates Thymocyte Migration

Ribeiro-Carvalho, Marilza Moura; Lima-Quaresma, K. R. F.; Mouço, T.; Carvalho, Vinícius Frias; Coelho, Valéria de Mello; Savino, Wilson

doi:10.1111/j.1365-3083.2007.01928.x

Cited by 9 publications

(5 citation statements)

References 57 publications

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“…Membrane expression of ECM receptors on cultured TECs - Single-cell suspensions of TECs were obtained after trypsin treatment as described and subjected to labelling with an FITC-coupled anti-CD49e or anti-CD49f antibody, as previously reported ( Ribeiro-Carvalho et al 2007 ). After washing, the cells were fixed in 0.5% formaldehyde and examined by flow cytometry.…”

Section: Methodsmentioning

confidence: 99%

Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection

Farias-de-Oliveira¹,

Cotta-de-Almeida²,

Villa‐Verde³

et al. 2013

Mem. Inst. Oswaldo Cruz

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Developing thymocytes interact with thymic epithelial cells (TECs) through cell-cell interactions, TEC-derived secretory moieties and extracellular matrix (ECM)-mediated interactions. These physiological interactions are crucial for normal thymocyte differentiation, but can be disrupted in pathological situations. Indeed, there is severe thymic atrophy in animals acutely infected with Trypanosoma cruzi due to CD4+CD8+ thymocyte depletion secondary to caspase-mediated apoptosis, together with changes in ECM deposition and thymocyte migration. We studied an in vitro model of TEC infection by T. cruzi and found that infected TEC cultures show a reduced number of cells, which was likely associated with decreased proliferative capacity, but not with increased cell death, as demonstrated by bromodeoxyuridine and annexin-V labelling. The infected TEC cultures exhibited increased expression of fibronectin (FN), laminin (LM) and type IV collagen. Importantly, treatment with FN increased the relative number of infected cells, whereas treatment with anti-FN or anti-LM antibodies resulted in lower infection rates. Consistent with these data, we observed increased thymocyte adhesion to infected TEC cultures. Overall, these results suggest that ECM molecules, particularly FN, facilitate infection of the thymic epithelium and that the consequent enhancement of ECM expression might be associated with changes in TEC-thymocyte interactions.

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Section: Methodsmentioning

confidence: 99%

Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection

Farias-de-Oliveira¹,

Cotta-de-Almeida²,

Villa‐Verde³

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…81 Moreover, systemic treatment or intrathymic injection of T 3 enhances thymocyte adhesion and migration towards extracellular matrix molecules. 82,83 Such biological effects occur via activation of T 3 nuclear receptors, which are expressed in both developing thymocytes and TECs. 84 Although the intrathymic production of thyroid hormone has not been reported, immunocytochemical data indicate that TSH can be produced by TECs, as detected by immunohistochemistry with β-TSH antibodies in the subcapsular and cortical thymic zones.…”

Section: Leptinmentioning

confidence: 99%

Hormonal control of T-cell development in health and disease

et al. 2015

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The physiology of the thymus, the primary lymphoid organ in which T cells are generated, is controlled by hormones. Data from animal models indicate that several peptide and nonpeptide hormones act pleiotropically within the thymus to modulate the proliferation, differentiation, migration and death by apoptosis of developing thymocytes. For example, growth hormone and prolactin can enhance thymocyte proliferation and migration, whereas glucocorticoids lead to the apoptosis of these developing cells. The thymus undergoes progressive age-dependent atrophy with a loss of cells being generated and exported, therefore, hormone-based therapies are being developed as an alternative strategy to rejuvenate the organ, as well as to augment thymocyte proliferation and the export of mature T cells to peripheral lymphoid organs. Some hormones (such as growth hormone and progonadoliberin-1) are also being used as therapeutic agents to treat immunodeficiency disorders associated with thymic atrophy, such as HIV infection. In this Review, we discuss the accumulating data that shows the thymus gland is under complex and multifaceted hormonal control that affects the process of T-cell development in health and disease.

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“…Triiodothyronine (T3), a thyroid hormone critical for the regulation of body metabolism, growth, and development, also exerts several effects on thymus physiology. Mice treated with T3 exhibited an increase in thymus weight and cellularity and an enhancement in the intrathymic expression of ECM and ECM receptors on thymocytes, including LM and VLA-6, respectively ( 92 – 94 ). Consequently, after short- and long-term T3 treatments, migration of thymocytes through LM is increased in ex vivo assays, proportionally to the treatment.…”

Section: Role Of Lms In Thymocyte Migrationmentioning

confidence: 99%

Laminin-Mediated Interactions in Thymocyte Migration and Development

et al. 2015

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Intrathymic T-cell differentiation is a key process for the development and maintenance of cell-mediated immunity, and occurs concomitantly to highly regulated migratory events. We have proposed a multivectorial model for describing intrathymic thymocyte migration. One of the individual vectors comprises interactions mediated by laminins (LMs), a heterotrimeric protein family of the extracellular matrix. Several LMs are expressed in the thymus, being produced by microenvironmental cells, particularly thymic epithelial cells (TECs). Also, thymocytes and epithelial cells express integrin-type LM receptors. Functionally, it has been reported that the dy/dy mutant mouse (lacking the LM isoform 211) exhibits defective thymocyte differentiation. Several data show haptotactic effects of LMs upon thymocytes, as well as their adhesion on TECs; both effects being prevented by anti-LM or anti-LM receptor antibodies. Interestingly, LM synergizes with chemokines to enhance thymocyte migration, whereas classe-3 semaphorins and B ephrins, which exhibit chemorepulsive effects in the thymus, downregulate LM-mediated migratory responses of thymocytes. More recently, we showed that knocking down the ITGA6 gene (which encodes the α6 integrin chain of LM receptors) in human TECs modulates a large number of cell migration-related genes and results in changes of adhesion pattern of thymocytes onto the thymic epithelium. Overall, LM-mediated interactions can be placed at the cross-road of the multivectorial process of thymocyte migration, with a direct influence per se, as well as by modulating other molecular interactions associated with the intrathymic-trafficking events.

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Triiodothyronine Modulates Thymocyte Migration

Cited by 9 publications

References 57 publications

Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection

Fibronectin modulates thymocyte-thymic epithelial cell interactions following Trypanosoma cruzi infection

Hormonal control of T-cell development in health and disease

Laminin-Mediated Interactions in Thymocyte Migration and Development

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