Triladyl® improves the cryopreserved quality and in vivo fertilization potential of Beetal buck (Capra hircus) spermatozoa

Shah, Syed Aftab Hussain; Haider, Muhammad Shafiq; Ahmed, Hussain; Fayyaz, Muhammad; Andrabi, S. M. H.

doi:10.1016/j.smallrumres.2023.106938

Cited by 3 publications

(2 citation statements)

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“…These chemicals can directly impact sperm movement and metabolic activity, thereby influencing the entire way of capacitation and fertilization [20]. Supplementing Triladyl® with 10% (v/v) chicken egg yolk enhances the cryopreserved in vitro and in vivo quality of Beetal buck spermatozoa when compared to a tris-citric acid extender containing 20% (v/v) egg yolk [21]. Sperm motility, kinematics, plasma membrane, acrosome, mitochondria and DNA integrities, as well as in vivo fertility (59.72% after single freezing versus 52.9% after double freezing; p > .05), remain acceptable following the double freezing protocol, although they are slightly lower than after a single freeze cycle in Beetal bucks [22].…”

Section: Discussionmentioning

confidence: 99%

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Effects of liquid storage of buck semen at refrigeration temperatures on sperm viability and fertility to develop ready to use goat semen diluent

Kumar,

Ranjan,

Bhardwaj

2024

J App Biol Biotech

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It is generally acknowledged that lower pH throughout liquid storage can decrease sperm motility and energy production, which may be advantageous for maintaining sperm viability during cryopreservation. Hence, the purpose of this study was to see how pH affected sperm viability, motility, membrane integrity, and oxidative changes after 5 days of storage in chemically specified extenders at refrigerator temperatures to formulate a ready-to-use goat semen diluent. Ejaculates were extended with tris-citric acid-fructose diluent with 20% or without egg yolk and stored at 5°C for 5 days. Sperm motility, live count, acrosome integrity, hypo-osmotic swelling positive spermatozoa, pH, and malondialdehyde all substantially differed significantly (P < 0.05) during different days of liquid storage. The pH of diluted semen decreased considerably (P < 0.05) from its original pH of 6.46 on 0 day to pH 6.06 on the 5th day of liquid storage at refrigeration temperature. Diluted semen at refrigeration temperature having 20% egg yolk had significantly higher (P < 0.05) seminal characteristics compared to liquid semen without egg yolk. The liquid storage of semen quality became worse day by day due to a drop in pH and egg yolks acted as a cryoprotectant against the harmful effects of chilling. Hence, 20% egg yolk may be used to enhance the keeping quality of diluted buck semen at refrigeration temperature for up to 5 days. Based on this result, we formulated a ready to use goat semen diluent for liquid semen storage at room temperature and refrigeration temperature.

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Section: Discussionmentioning

confidence: 99%

“…AI plays a significant role in improving feasibility and enhancing economic efficiency. Fresh or well-preserved semen is required for effective AI, with approximately 95% of all, AI utilizing preserved semen [9]. Therefore, the preservation medium is crucial to maintaining semen quality.…”

Section: Introductionmentioning

confidence: 99%