2023
DOI: 10.1186/s13046-022-02583-z
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TRIM21 attenuates renal carcinoma lipogenesis and malignancy by regulating SREBF1 protein stability

Abstract: Background Metabolic reprogramming is a hallmark of various cancers. Targeting metabolic processes is a very attractive treatment for cancer. Renal cell carcinoma (RCC) is a type of metabolic disease, and the lipidomic profile of RCC is significantly altered compared with that of healthy tissue. However, the molecular mechanism underlying lipid metabolism regulation in RCC is not clear. Methods The XF long-chain fatty acid oxidative stress test kit… Show more

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Cited by 10 publications
(4 citation statements)
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“…Seahorse Bioscience Extracellular Flux Analyzer (XF96, Seahorse Bioscience Inc., North Billerica, MA, USA) was used to monitor glycolysis and mitochondrial function by measuring extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) as described in manufacturer’s instructions. Glucose uptake and lactate production were performed using the glucose assay kit (Shanghai Rongsheng Biotech, Shanghai, China) and lactate assay kit (Shanghai Rongsheng Biotech, Shanghai, China) following the manufacturer’s instructions as previously described [ 21 , 22 ], the detailed procedure was described in Supplementary Materials and Methods .…”
Section: Methodsmentioning
confidence: 99%
“…Seahorse Bioscience Extracellular Flux Analyzer (XF96, Seahorse Bioscience Inc., North Billerica, MA, USA) was used to monitor glycolysis and mitochondrial function by measuring extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) as described in manufacturer’s instructions. Glucose uptake and lactate production were performed using the glucose assay kit (Shanghai Rongsheng Biotech, Shanghai, China) and lactate assay kit (Shanghai Rongsheng Biotech, Shanghai, China) following the manufacturer’s instructions as previously described [ 21 , 22 ], the detailed procedure was described in Supplementary Materials and Methods .…”
Section: Methodsmentioning
confidence: 99%
“…IHC assays were conducted following the standard streptavidin-peroxidase method, as previously reported [ 28 ]. The primary antibodies used were as follows: anti-PRMT2 1:100 dilution, anti-WNT5A 1:100 dilution, anti-Ki-67 1:100 dilution, and anti-Cyclin-D1 1:100 dilution.…”
Section: Methodsmentioning
confidence: 99%
“…Renal cancer tissue microarray (TMA) slides and corresponding patients’ clinicopathological information were obtained according to a previous report [ 28 ]. The detailed patient’s clinicopathological information was shown in Supplementary Information.…”
Section: Methodsmentioning
confidence: 99%
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