TRIM24 promotes the aggression of gastric cancer via the Wnt/β-catenin signaling pathway

Abstract

Abstract. Tripartite motif-containing 24 (TRIM24) is important in tumor development and progression. However, the role of TRIM24 in gastric cancer (GC) and the mechanisms underlying the dysregulated expression of TRIM24 remain to be fully elucidated. In the present study, it was found that TRIM24 was frequently overexpressed in GC cell lines and tissues compared with normal controls, as determined by western blotting and immunohistochemical staining. The high nuclear expression of TRIM24 was correlated with the depth of invasion (P=0.007), tumor-node-metastasis stage (P=0.005), and lymph node metastasis (P=0.027), and shorter overall survival rates (P=0.010) in patients with GC. Small interfering RNA-mediated knockdown of TRIM24 inhibited cell proliferation, colony formation, migration, invasion and the nuclear accumulation of β-catenin, and it delayed cell cycle progression and induced apoptosis. In addition, the expression of TRIM24 was positively correlated with that of β-catenin in GC tissues. TRIM24 knockdown decreased the expression of Wnt/β-catenin target genes, whereas the activation of Wnt/β-catenin signaling by lithium chloride reversed the effects of TRIM24 knockdown. Taken together, these data suggested that TRIM24 was a prognostic or potential therapeutic target for patients with GC and was important in the activation of the Wnt/β-catenin pathway during the progression of GC.

Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/β-catenin axis

“…As for TRIM24, it is a member of the tripartite motif (TRIM) family [20], and the oncogenic role of TRIM24 in carcinogenesis has been demonstrated in several cancer types [43][44][45]. Moreover, our study identified that inhibited miR-138-2-3p or promoted TRIM24 stimulated Wnt/β-catenin activation in glioma cells, which was in line with the previous studies [21,41]. Wnt/β-catenin controls diverse cellular processes and drives cancer progression [46], with its activation revealed to promotes tumorigenesis of multiple cancers [47,48], including glioma [23].…”

“…TRIM24 is a member of the TRIM family with its oncogenic role being identified in prostate cancer [20]. In addition, TRIM24 has been suggested to promote cancer progression by triggering the Wnt/β-catenin signaling pathway [21,22]. This pathway has been noted to be closely associated with cellular proliferation, migration, and angiogenesis and the following glioma malignancy [23].…”

Identification of key genes and pathways downstream of the β‑catenin‑TCF7L1 complex in pancreatic cancer cells using bioinformatics analysis

“…For example, certain studies have identified that TRIM24 is overexpressed, and promotes cancer cell growth and invasion in bladder cancer and cervical cancer, possibly via the nuclear factor-κB and PI3K/Akt signaling pathways (36,37). Similarly, it has been reported that TRIM24 can accelerate cell growth and facilitate gastric cancer progression by activation of the Akt pathway (37) and the Wnt/β-catenin signaling pathway (38). Notably, in contrast to the aforementioned studies that suggest TRIM24 is an important oncogene in tumor development, TRIM24 has been identified to suppress the progression of murine hepatocellular carcinoma (39).…”

<em>TRIM44</em>, a crucial target of miR-410, functions as a potential oncogene in osteosarcoma

“…Immunohistochemistry was performed as previously described. 21 Briefly, slides went through antibody incubation overnight at 4°C with TRIM44 antibody (11511-1-AP; Proteintech, Chicago, IL, USA). The staining scores were then evaluated by two independent pathologists.…”