Trimethyl-ε-caprolactone synthesis with a novel immobilized glucose dehydrogenase and an immobilized thermostable cyclohexanone monooxygenase

Baeyer–Villiger Monooxygenases: Tunable Oxidative Biocatalysts

“…On a 100 L scale, the 2.58 kg obtained material contained 84.5% product, and the final yield was 76%. 331 Although the biocatalyst yield (gram product per gram enzyme/ cells) seems much lower compared with the same reaction with immobilized enzyme, 330 it is actually difficult to compare these values, since a lot of costs go into the preparation and immobilization of the enzymes, in contrast to the fermentation broth used in this study. 331 This demonstration is a great example of how to reach large scale production with a BVMO reaction by addressing important challenges, in particular the oxygen limitation through careful supply of pure oxygen.…”

“…Compared with the soluble TmCHMO and GDH, the immobilized forms had a 3.6-fold and 1.9-fold improvement, respectively, in terms of the biocatalyst yield (37.3 g g −1 for TmCHMO and 474.2 g g −1 for GDH). 330 To investigate the relevance of these biocatalytic studies on 3,3,5trimethylcyclohexanone conversion in terms of applicability and environmental impact, a life-cycle analysis (LCA) was done. 314 The biocatalytic process 327 was compared with a chemical synthesis route, and no clear difference in climate change impact was found between the two routes.…”

A multistep (semi)-continuous biocatalytic setup for the production of polycaprolactone

“…Immobilizing the ADH and BVMO enzymes would make the filtration of the feed not necessary as the solution would be free of debris. Few studies have reported successful BVMO immobilization methods, 6,38,39 and so far the increased mass transfer limitations have hindered the productivity of these systems. As a result, the reaction times to achieve a comparable conversion to the free enzyme system are too long for it to be a viable alternative, at least until an improved immobilization system is developed.…”