Trimethyltin chloride induces apoptosis and DNA damage via ROS/NF-κB in grass carp liver cells causing immune dysfunction

Li, Lulu; Gao, Meichen; Yang, Naixi; Ai, Liwen; Guo, Liyang; Xue, Xuexue; Sheng, Zunlai

doi:10.1016/j.fsi.2023.109082

Cited by 7 publications

(1 citation statement)

References 63 publications

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“…CASP3 is a member of the Caspase family, which is located downstream of the cascade reaction of Caspas e and is considered to be the only pathway in the apoptosis cascade reaction. Its activation marks the entry of the cell into an irreversible stage of apoptosis [ 56 ]. After liver injury, cells may experience severe endoplasmic reticulum stress, leading to excessive activation of Caspase, inhibition of PI3K and Akt acidification, reduction of HIF-1 mTOR expression, disruption of redox homeostasis and energy balance, and promotion of cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Arecoline-Induced Hepatotoxicity in Rats: Screening of Abnormal Metabolic Markers and Potential Mechanisms

Sun,

Zhang,

Yin

et al. 2023

Toxics

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Arecoline is a pyridine alkaloid derived from areca nut in the Arecaceae family. It has extensive medicinal activity, such as analgesic, anti-inflammatory, and anti-allergic. However, the toxicity of Arecoline limits its application. Most current studies on its toxicity mainly focus on immunotoxicity, carcinogenesis, and cancer promotion. However, there are few systematic studies on its hepatotoxicity and mechanisms. Therefore, this research explored the mechanism of hepatotoxicity induced by Arecoline in rats and analyzed endogenous metabolite changes in rat plasma by combining network toxicology with metabolomics. The differential metabolites after Arecoline exposure, such as D-Lysine, N4-Acetylaminobutanal, and L-Arginine, were obtained by metabolomics study, and these differential metabolites were involved in the regulation of lipid metabolism, amino acid metabolism, and vitamin metabolism. Based on the strategy of network toxicology, Arecoline can affect the HIF-1 signaling pathway, MAPK signaling pathway, PI3K-Akt signaling pathway, and other concerning pathways by regulating critical targets, such as ALB, CASP3, EGFR, and MMP9. Integration of metabolomics and network toxicology results were further analyzed, and it was concluded that Arecoline may induce hepatotoxicity by mediating oxidative stress, inflammatory response, energy and lipid metabolism, and cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Arecoline-Induced Hepatotoxicity in Rats: Screening of Abnormal Metabolic Markers and Potential Mechanisms

Sun,

Zhang,

Yin

et al. 2023

Toxics

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Exposure to Trimethyltin Chloride Induces Pyroptosis and Immune Dysfunction in Grass Carp CIK Cells by Activating the NF‐κB Pathway Through Oxidative Stress

Ni,

Hong,

et al. 2024

Environmental Toxicology

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Trimethyltin chloride (TMT) is a highly toxic organotin pollutant frequently found in aquatic environments, posing a significant threat to the ecological system. The kidney plays a vital role in the body's detoxification processes, and TMT present in the environment tends to accumulate in the kidneys. However, it remained unclear whether exposure to different doses of TMT could induce pyroptosis and immune dysfunction in grass carp kidney cells (CIK cells). For this purpose, after assessing the half‐maximal inhibitory concentration (IC50) of TMT on CIK cells, we established a model for exposure of CIK cells at varying concentrations of TMT. CIK cells were treated with various doses of TMT (2.5, 5, 10 μM) for 24 h. Oxidative stress levels were measured using kits and fluorescence methods, whereas the expression of related genes was verified through western blot and quantitative real‐time PCR (qRT‐PCR). The results indicated that TMT exposure led to oxidative stress, with increased levels of ROS, H2O2, MDA, and GSH, and inhibited activities of T‐AOC, SOD, and CAT. It activated the NF‐κB pathway, leading to the upregulation of NF‐κB p65, NF‐κB p50, GSDMD, NLRP3, ASC, and Caspase‐1. Furthermore, TMT exposure also resulted in increased expression of cytokines (IL‐18, IL‐6, IL‐2, IL‐1β, and TNF‐α) and decreased expression of antimicrobial peptides (LEAP2, HEPC, and β‐defensin). In summary, exposure to TMT induces dose‐dependent oxidative stress that activates the NF‐κB pathway, leading to pyroptosis and immune dysfunction in grass carp CIK cells.

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Organotin(IV) from Simple Complexes to Macromolecules: A Review Inspired by the Late Professor Charles Carraher

Momeni,

Abd-El-Aziz,

et al. 2024

J Inorg Organomet Polym

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Trimethyltin chloride induces apoptosis and DNA damage via ROS/NF-κB in grass carp liver cells causing immune dysfunction

Cited by 7 publications

References 63 publications

Arecoline-Induced Hepatotoxicity in Rats: Screening of Abnormal Metabolic Markers and Potential Mechanisms

Arecoline-Induced Hepatotoxicity in Rats: Screening of Abnormal Metabolic Markers and Potential Mechanisms

Exposure to Trimethyltin Chloride Induces Pyroptosis and Immune Dysfunction in Grass Carp CIK Cells by Activating the NF‐κB Pathway Through Oxidative Stress

Organotin(IV) from Simple Complexes to Macromolecules: A Review Inspired by the Late Professor Charles Carraher

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