Triology of nitric oxide, mast cell and stress in pathogenesis of oral lichen planus

Abstract

Introduction:Oral Lichen planus (OLP) is chronic, autoimmune, mucocutaneous disease. Numerous etiological factors have been proposed, but an authoritative and exact source of the disease has not been brought forward. Reactive nitrogen species (RNS), mast cell (MC) and stress are considered to play a key role in inflammation-mediated carcinogenesis generating nitric oxide (NO).Aim:To evaluate the salivary NO levels, mast cells and stress levels and to correlate them in pathogenesis of OLP.Materials and Methods:The study was conducted using saliva samples of patients. The study consisted of two groups: Group-I constituted the subjects with OLP group (n=25) and group II comprised the control group (n=25). The saliva of the patients was evaluated using Griess Reagent and Spectrophotometer, MC count done by using special stains, and stress levels measured using DASS Scale.Results:The difference between the means was found to be highly significant (P < 0.05). The intergroup comparison of optical density (OD) values, a mast cell count and stress level was found to be highly significant.Conclusion:Salivary NO, increase in mast cell count and stress has a definitive role in OLP pathogenesis.

Serum Vitamin D Level In Oral Lichen Planus Patients Of North India- A Case-Control Study.

“…3 In general population, the prevalence of OLP is about 0.5%-2.2%. It also restricts antigen presentation via macrophages and modulates dendritic cells maturation.…”

Association of nitric oxide with oral lichen planus

“…All of the included studies were case‐control in nature and 151 patients with OLP were evaluated (mostly females), with an age ranged from 20 to 75 years. Of the seven included studies, four were from India, and one each from Spain, Iran and Japan . The majority of studies used whole unstimulated saliva and one study used unstimulated gland saliva from parotid and submandibular glands collection of NO .…”

“…Since NO is so reactive, it cannot be measured directly and detection is by its reaction products. Griess assay was the most common method used for the analysis of NO . Only one study used serum and ELISA kit for the extraction and analysis of NO .…”

“…The NOS score for the quality of the included studies ranged from 6 to 7. Only two studies had a NOS score of “7” to be considered good‐quality studies, while the remaining five studies recorded a NOS score of “6.” The majority of studies scored high in the selection domain and comparability domain was the least scored domain. The majority of studies also scored high in the outcome/exposure domain.…”

“…While assessing quality of the included studies using NOS score, only two studies were classified as good quality . Both of these reported OLP cases that were confirmed either through clinical and/or histopathological analysis, had consecutive representative series of cases and controls with no history of disease.…”