Tripartite interactions among Paenibacillus lentimorbus NRRL B-30488, Piriformospora indica DSM 11827, and Cicer arietinum L.

Abstract

Tripartite interactions among Paenibacillus lentimorbus NRRL B-30488 (B-30488), Piriformospora indica DSM 11827 (DSM 11827) and their consortia (B-30488:DSM 11827:: 1:1) with native rhizobial population in the rhizosphere of Cicer arietinum L. (Chick pea) was tested for enhancing nodulations and plant growth promotion. Number of nodules and dry weight per plant significantly enhanced (P = 0.05), which is further evident by N, P, and K uptake by plants and were found to be maximum in B-30488 treated followed by B-30488: DSM 11827 and DSM 11827, as compared with uninoculated control, in 60 days grown chickpea plants. Microbial community structure in the rhizosphere of the four treatments was assessed, using Biolog Eco and MT plates. Principal component analysis (PCA) of carbon source utilization pattern on Biolog Eco plates did not show any clustering among the four samples indicating that in case of individually DSM 11827 and B-30488 treated chickpea rhizosphere there was significant change in microbial community structure, compared with lesser changes in uninoculated and B-30488 and DSM 11827 consortium treated chickpea rhizosphere microflora. Additional carbon sources tested using Biolog MT plates, higher activity of lignin, chitin, and cellulose utilizing microbial communities in the rhizosphere being stimulated by root exudates treated with B-30488 alone or in consortia with DSM 11827, and, in turn, should encourage beneficial symbiotic or mutualistic microorganisms that can act as plant growth promoting and biocontrol agents.

Impact of Culture Filtrate of Piriformospora indica on Biomass and Biosynthesis of Active Ingredient Aristolochic Acid in Aristolochia elegans Mart

“…Stem height increased by 43% and shoot length by 155%.This is in conformity to observations made in earlier investigations (Varma et al, 2001;Nautiyal et al, 2010;Bagde et al, 2010aBagde et al, , 2011.…”

Bioaugmentation of Mesorhizobium cicer, Pseudomonas spp. and Piriformospora indica for Sustainable Chickpea Production

“…Data are average value of three plants and following the analysis of variance (ANOVA) procedure, differences between desi PBG1 and kabuli BG1053 chickpea varieties, among different treatments and between varieties and treatments were determined using Critical difference from soil by native AMF and inoculated P. indica also might have benefited in development of efficient nodules in chickpea. Our results are in congruence with findings of Nautiyal et al (2010) who also demonstrated consortia of P.indica and PGPR enhanced the nodulation ability by native rhizobia in chickpea. On the contrary, Tavasolee et al (2011) reported that PGPR as bacterial strains have lowered nodule fresh mass due to competition for photosynthetic matter between bacteria and fungi in chickpea.…”

“…Significantly high N content was recorded in MR+PI+LPGPR1 (1.79 % and 1.85 %) followed byMR + PI + LK884 (1.77 % and 1.80 %) and MR+PI+LPGPR2 (1.73 % and 1.74 %) in desi PBG1 and kabuli BG1053, respectively over MR alone treatment. Improvement in total N content is well in line with previous findings in chickpea (Nautiyal et al 2010) and soybean (Lima et al 2011) where different bio-inoculant combinations as P.indica+PGPR, AMF+Azospirillum and Bradyrhizobium sp.+Glomus etunicatum, respectively. Higher P content was observed in P. indica alone treatment as compared to Mesorhizobium cicer alone.…”

“…In congruence with this, significantly high P content was recorded in MR+PI+LPGPR1 treatment (0.218 and 0.216 %) followed by MR+PI+LK884 (0.203 and 0.205 %) and MR+PI+LPGPR2 (0.191 and 0.195 %) in desi PBG1and in kabuli BG1053 chickpea varieties, respectively. The investigation has been found coherent between the results of Nautiyal et al (2010) who reported that consortia of P. indica and PGPR along with native chickpea rhizobia increased the P content. …”

Phosphate utilization by the fungal root endophyte Piriformospora indica

“…Despite this strong indication of a direct contribution of P. indica to plant P nutrition, experimental results on agricultural crops have been inconsistent. While in tobacco, barley and green gram, colonization by P. indica did not increase P content of plants despite plant growth promotion (Barazani et al 2007;Achatz et al 2010;Ray and Valsalakumar 2010), chickpea and black lentil plants showed higher P content (Nautiyal et al 2010;Kumar et al 2012). This inconsistency may be partly explained by differences in experimental conditions (Fakhro et al 2010) and highlights the need for further research.…”