Triphenyltetrazolium Chloride as a Dye for Vital Tissues

Mattson, A. M.; Jensen, C. O.; Dutcher, R. A.

doi:10.1126/science.106.2752.294.b

Cited by 27 publications

(11 citation statements)

References 7 publications

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“…UV-vis experiments including analyses of the diafiltered solutions were performed in a Heλios γ spectropho-tometer. 1 H NMR measurements were made in a JNM-Lambda500 (JEOL, 500 MHz) and in an AVANCE600 (Bruker, 600 MHz) spectrometers.…”

Section: Reagentsmentioning

confidence: 99%

“…UV-vis spectrophotometric measurements of the TTC-PSS mixtures were made with a solution of PSS of the same concentration as the reference. Standard methods have been used for 1 Redox kinetic studies were carried out by successively adding into a quartz vessel 1 mL of a 6 × 10 -3 M TTC aqueous solution, 1 mL of a 6 × 10 -2 M WSP solution, 0.5 mL of a 1.2 × 10 -1 M ASC solution, and 0.5 mL of a 1 M NaOH solution. After shaking for 2 s, the reactions were followed by UV-vis spectroscopy at 524 nm.…”

Section: Reagentsmentioning

confidence: 99%

“…2,3,5-Triphenyl-2H-tetrazolium chloride (TTC) is an interesting molecule used as indicator in various biological applications, such as counting bacterial colonies, indicating viability of seeds, differential staining of tissues, or bacteriostatic action. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] Its use for these purposes is based on its ability to reduce to the corresponding red formazan. This redox-based staining property serves to evaluate reductive enzyme activity, to oxidize sugars and steroids, and to photometrically determine metal activities.…”

Section: Introductionmentioning

confidence: 99%

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Aromatic−Aromatic Interaction between 2,3,5-Triphenyl-2H-tetrazolium Chloride and Poly(sodium 4-styrenesulfonate)

et al. 2007

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Aromatic-aromatic interactions are found between the cationic molecule 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) and the molecule poly(sodium 4-styrenesulfonate) (PSS) which makes the overall interaction of TTC with PSS more intense than the interaction with other polyanions containing sulfonate groups and produces a decrease on the redox ability of TTC. Diafiltration was used to compare the binding of TTC to PSS, poly(sodium vinylsulfonate) (PVS), and the more hydrophobic poly(sodium 2-(N-acrylamido)-2-methyl-propanesulfonate) (PAMPS). The UV-vis spectrum of TTC is changed in the presence of the aromatic polyanion. The 1H NMR signals of TTC are broadened and shifted in the presence of PSS, suggesting the occurrence of pi-pi interactions. Moreover, nuclear Overhauser effects (NOE) between the TTC and PSS protons are found. Possible structures for the complex are proposed.

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Section: Reagentsmentioning

confidence: 99%

Section: Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aromatic−Aromatic Interaction between 2,3,5-Triphenyl-2H-tetrazolium Chloride and Poly(sodium 4-styrenesulfonate)

et al. 2007

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“…On the contrary, strains that have less intracellular NADH, namely weak glycolysis flow, cannot reduce TTC to TPF and will exhibit a white color. Since aneuploids have physiologies different from euploids [20], we hypothesized that white colonies are candidates for the strains, which have weak glycolysis flow and are aneuploids. Even if the developed strains have low ethanol productivity, novel brewery characteristics might compensate for the disadvantage of low ethanol productivity.…”

Section: Introductionmentioning

confidence: 99%

Induction of Chromosomal Aneuploids from Brewery Shochu Yeast with Novel Brewery Characteristics

et al. 2022

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The first development method of brewery shochu yeast focusing on chromosomal aneuploidy is reported in this study. Euploidy diploid shochu yeast S-3 was treated with a microtubule inhibitor, nocodazole, for the purpose of inducing aneuploidy. Next, 2,3,5-triphenyl tetrazolium chloride (TTC) staining and the growth rate were investigated to select aneuploids. Aneuploids were selected at a frequency of 8.2 × 10−4, which was significantly higher than that of the control group, mainly at chromosomes I, II, III, IX, XII, XIII, and XVI. The acquired aneuploids were evaluated for their metabolic and brewing characteristics. A hierarchical cluster analysis based on endogenous metabolite data discriminated euploid S-3 and aneuploids. In addition, principal-component analysis of the constituents of the broth brewed with the strains discriminated between euploid S-3 and aneuploids. Sensory evaluation of the broth brewed with euploid S-3 and aneuploids showed that it tended to differ in aroma and taste. Specific ethanol production rates of the aneuploids were not deteriorated. The method of this selection made it possible to efficiently obtain aneuploids with various brewing characteristics from brewer’s yeast, which do not correspond to genetically modified organisms. This novel breeding method focusing on chromosomal aneuploidy will facilitate the development of novel shochu yeast strains.

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“…This property of formazans has found application in various fields of science and engineering. They have been used in botany as colouring compounds sensitive to ultraviolet radiation, 1 as substances suitable for estimating the productivity of seeds of agricultural plants 2,3 and the viability of stem and leaf tissues, 4,5 for distinguishing between normal and malignant tissues, 6 and for studying the histochemistry of oxidative enzymes. 7,8 The use of formazans as analytical reagents for detection of O À 2 radical ions has also been reported.…”

Section: Introductionmentioning

confidence: 99%