Triphenyltetrazolium Chloride as a Dye for Vital Tissues

Aromatic-aromatic interactions are found between the cationic molecule 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) and the molecule poly(sodium 4-styrenesulfonate) (PSS) which makes the overall interaction of TTC with PSS more intense than the interaction with other polyanions containing sulfonate groups and produces a decrease on the redox ability of TTC. Diafiltration was used to compare the binding of TTC to PSS, poly(sodium vinylsulfonate) (PVS), and the more hydrophobic poly(sodium 2-(N-acrylamido)-2-methyl-propanesulfonate) (PAMPS). The UV-vis spectrum of TTC is changed in the presence of the aromatic polyanion. The 1H NMR signals of TTC are broadened and shifted in the presence of PSS, suggesting the occurrence of pi-pi interactions. Moreover, nuclear Overhauser effects (NOE) between the TTC and PSS protons are found. Possible structures for the complex are proposed.

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Aromatic−Aromatic Interaction between 2,3,5-Triphenyl-2H-tetrazolium Chloride and Poly(sodium 4-styrenesulfonate)

Moreno‐Villoslada

González

Rivera

et al. 2007

J. Phys. Chem. B

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Molecular assay for detecting MS-H vaccine strain and immune response mechanisms in chickens receiving one or two doses of live MS-H vaccine

Limpavithayakul,

Charoenvisal,

Pakpinyo

2023

Avian Pathology

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Triphenyltetrazolium Chloride as a Dye for Vital Tissues

Cited by 2 publications

References 7 publications

Aromatic−Aromatic Interaction between 2,3,5-Triphenyl-2H-tetrazolium Chloride and Poly(sodium 4-styrenesulfonate)

Aromatic−Aromatic Interaction between 2,3,5-Triphenyl-2H-tetrazolium Chloride and Poly(sodium 4-styrenesulfonate)

Molecular assay for detecting MS-H vaccine strain and immune response mechanisms in chickens receiving one or two doses of live MS-H vaccine

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