Triphenyltin recognition by primary structures of effector proteins and the protein network of Bacillus thuringiensis during the triphenyltin degradation process

Abstract

Herein, triphenyltin (TPT) biodegradation efficiency and its transformation pathway have been elucidated. To better understand the molecular mechanism of TPT degradation, the interactions between amino acids, primary structures, and quaternary conformations of effector proteins and TPT were studied. The results verified that TPT recognition and binding depended on amino acid sequences but not on secondary, tertiary or quaternary protein structure. During this process, TPT could change the molecular weight and isoelectric point of effector proteins, induce their methylation or demethylation, and alter their conformation. The effector proteins, alkyl hydroperoxide reductase and acetyl-CoA acetyltransferase, recognizing TPT were crucial to TPT degradation. Electron transfer flavoprotein subunit alpha, phosphoenolpyruvate carboxykinase, aconitate hydratase, branched-chain alpha-keto acid dehydrogenase E1 component, biotin carboxylase and superoxide dismutase were related to energy and carbon metabolism, which was consistent with the results in vivo. The current findings develop a new approach for investigating the interactions between proteins and target compounds.

Leucine Supplementation Modulates Lipid Metabolism and Inflammation in Early Weaning Piglets

“…48 ACAT2 indirectly promotes the citrate pyruvate cycle, which is involved in fatty acid synthesis. 49 In summary, early weaning increased the expressions of FASN, ACACB, and ACAT2 and decreased expressions of ACOT1, ACOT2, PPARα, and the activity of hepatic fatty acid β-oxidation enzymes thereby increasing the risk of body fat deposition. However, the addition of leucine does not improve fat deposition to normal weaning levels in the short term.…”

“…Research has shown that mice lacking ACACB exhibit increased rates of fatty acid oxidation and resistance to high-fat diets . ACAT2 indirectly promotes the citrate pyruvate cycle, which is involved in fatty acid synthesis . In summary, early weaning increased the expressions of FASN , ACACB , and ACAT2 and decreased expressions of ACOT1 , ACOT2 , PPARα , and the activity of hepatic fatty acid β-oxidation enzymes thereby increasing the risk of body fat deposition.…”

Molecular Recognition and Cell Surface Biochemical Response of Bacillus thuringiensis on Triphenyltin

“…Although the mechanical properties vary among the phenyltins used in this study (Figure 3b), there was no correlation between adhesion force, molecular weight, molecular size, and twist angle (Table S3), which demonstrates that the changes in the cells' mechanical properties are dependent on the interaction between the pollutants and the cells. It has been verified that the recognition and binding of TPT by functional proteins depend on a protein's specific amino acid sequences but not its advanced structure [26]. Organisms that contain the same genes or proteins exhibit the same or similar responses to TPT, and the effective biosorption, transport, and degradation of TPT by Brevibacillus brevis support the above deduction [7].…”