Triple mass spectrometry (MS/MS/MS) with a floated collision cell in a four‐sector tandem mass spectrometer

Wu, Zheng; Bordas-Nagy, Joseph; Fenselau, Catherine

doi:10.1002/oms.1210261019

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…Mu ltiple-stage mass spectrometry (MS", II > 2) is a very useful technique for studies of internal energy deposition and fragmentation pathways [37,38] and has been used in structural mass spectrometry as well [39,40]. MS 4 and MS s have been demonstrated in a four-sector tandem instrument with high energy ClO [38].…”

Section: S 4 Experim Entsmentioning

confidence: 99%

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Interface for a four-sector mass spectrometer with a dual-purpose collision cell: High transmission at low to intermediate energies

Cheng

Fenselau

et al. 1995

J. Am. Soc. Mass Spectrom.

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A new interface system that consists of an ion decelerator, a floating collision cell-chemical ionization ion source, and an ion extractor was designed and installed in the third field-free region of a four-sector tandem mass spectrometer. Important features include the use of cylindrical deceleration lenses and an extraction lens assembly. This new design was found to provide enhancement of ion transmission at low to intermediate ion kinetic energies (3 eV to 1 keV) compared with the standard collision cell design. Collision-induced dissociation experiments from 3 eV to 10 keV and ion-molecule reactions of mass-selected ions can be performed conveniently. A second, grounded, collision cell is located after the extraction lenses, which allows MS(4) experiments to be carried out via the normal linked (B/E) scan function in MS2. Incorporation of chemical ionization capability into the electrically isolated collision cell makes it possible to carry out neutralization chemical-reionization mass spectrometry.

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Section: S 4 Experim Entsmentioning

confidence: 99%

“…MS 4 and MS s have been demonstrated in a four-sector tandem instrument with high energy ClO [38]. In our previous MS:l experiment [40], successive CIO was completed by using two collis ion cells located in the first and third FFR, respectively. By floating the second collision cell, sensitivity for low mass ions was enhanced.…”

Section: S 4 Experim Entsmentioning

confidence: 99%

Interface for a four-sector mass spectrometer with a dual-purpose collision cell: High transmission at low to intermediate energies

Cheng

Fenselau

et al. 1995

J. Am. Soc. Mass Spectrom.

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“…where U; = Utly, and m~= mf/Y. Because electrostatic and magnetic sectors separate ions according to their kinetic energy to charge ratio (U;) and mass-tocharge ratio (m:), respectively, the initial setting of MS-II for B/E linked scan should follow the equation [10,12], where V 2 is the initial voltage of M5-II. In these experiments, V 2 is always set at 10 kV.…”

Section: Methodsmentioning

confidence: 99%

High-energy collision-induced dissociation of multiply charged polypeptides produced by electrospray

Fabris

Kelly

Murphy

et al. 1993

J. Am. Soc. Mass Spectrom.

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The recent commercial implementation of an electrospray source on a four-sector mass spectrometer has allowed the study of high-energy collisional activation of multiply charged cations. With this configuration, higher mass-to-charge ratios can be accommodated in both precursor ion selection and fragment ion detection. Good mass accuracy facilitates analysis of fragment ions and allows more reliable mechanistic correlation of these fragments. A convenient scheme was devised to permit the use of kilovolt potentials in both MS-I and MS-II, with precursors of varying charge states. Algorithms were devised to assign masses of different types of multiply charged fragment ions. Nine polypeptides were studied in the mass range 2000-5000 Da. Through this entire mass range, fragment ions were observed to be amply formed by cleavages in both the backbone and side chains, analogous to high-energy collisional activation of singly charged ions. This stands in sharp contrast to the patterns reported with low-energy, multiple collisions. Abundances of sequence ion series are influenced by the positions of basic residues. Analysis of charge distributions in fragment ions also indicates that the charges tend to be spread out across the peptides.

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“…Typically, the tandem double-focusing mass spectrometers have been used for determining the product ions of a single mass-selected precursor ion (product ion scan). A few unique experiments have been performed on tandem double-focusing mass spectrometers, but these experiments involve scanning only one of the double-focusing mass spectrometers. − …”

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confidence: 99%

Synchronized Scanning of the First and Second Mass Spectrometers on Tandem Double-Focusing Mass Spectrometers

Strobel

1997

Anal. Chem.

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A method is described to synchronize the scans of the first and second double-focusing mass spectrometers of a tandem double-focusing mass spectrometer. The scans are synchronized by scanning the first mass spectrometer with voltages instead of the magnetic field. The method is demonstrated with scans that provide all of the precursor ions that produce a selected product ion (precursor ion scan). These precursor ion scans are compared to the previous method of obtaining precursor ion scans on a tandem double-focusing mass spectrometer. The precursor ion scans using the synchronized scanning allow for the use of high-energy collisions on any tandem double-focusing mass spectrometer, while limiting the mass range possible in a single scan. The limited mass range may be corrected by obtaining several spectra with different magnetic fields on the first mass spectrometer.

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Triple mass spectrometry (MS/MS/MS) with a floated collision cell in a four‐sector tandem mass spectrometer

Cited by 6 publications

References 8 publications

Interface for a four-sector mass spectrometer with a dual-purpose collision cell: High transmission at low to intermediate energies

Interface for a four-sector mass spectrometer with a dual-purpose collision cell: High transmission at low to intermediate energies

High-energy collision-induced dissociation of multiply charged polypeptides produced by electrospray

Synchronized Scanning of the First and Second Mass Spectrometers on Tandem Double-Focusing Mass Spectrometers

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