Triple-recognition strategy for one-pot detection of single nucleotide variants by aligner-mediated cleavage-triggered exponential amplification

Long, Jinyan; Chen, Mengqi; Yu, Yanyan; Wu, Qiaomin; Yang, Xiaolan

doi:10.1016/j.aca.2023.341617

Cited by 3 publications

(1 citation statement)

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“…Long et al. proposed a triple-recognition strategy, which promotes aligner-mediated cleavage-triggered EXPAR for the specific SNV assay [ 82 ]. In the study, the authors designed a hairpin-shaped DNA aligner with two side arms, which are complementary to the MT strand with a G-mutation (SNV) and WT strand.…”

Section: Homogeneous Snv Assays In the Test Tubementioning

confidence: 99%

Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

Xiong,

Liu,

Deng

et al. 2024

National Science Review

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Single-nucleotide variants (SNVs) are the most common type variation of sequence alterations at a specific location in the genome, thus involving significant clinical and biological information. The assay of SNVs has engaged great awareness, because many genome-wide association studies demonstrated that SNVs are highly associated with serious human diseases. Moreover, the investigation of SNV expression level in single cells are capable of visualizing genetic information and revealing the complexity and heterogeneity of single-nucleotide mutations-related diseases. Thus, developing SNV assay approaches in vitro, particularly in single cells, is in increasing demand. In this review, we summarized recent progress in the enzyme-free and enzyme-mediated strategies enabling SNV assay transition from sensing interface to the test tube and single cells, which will potentially delve deeper into the knowledge of SNV functions and disease associations, as well as discover new pathways to diagnose and treat diseases based on individual genetic profiles. The leap of SNV assay achievements will motivate observation and measurement genetic variations in single cells, even within living organisms, delve into the knowledge of SNV functions and disease associations, as well as open up entirely new avenues to diagnose and treat diseases based on individual genetic profiles.

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Section: Homogeneous Snv Assays In the Test Tubementioning

confidence: 99%