Triple Signal Amplification Strategy for Ultrasensitive Determination of miRNA Based on Duplex Specific Nuclease and Bridge DNA–Gold Nanoparticles

Bo, Bing; Zhang, Tian; Jiang, Yijie; Cui, Haiyan; Miao, Peng

doi:10.1021/acs.analchem.7b05447

Cited by 107 publications

(53 citation statements)

References 41 publications

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“…Bare AuNPs were synthesized according to a previous report. [28] 3 µL of thiolated Probe a (100 × 10 −6 m) was added in 100 µL of AuNPs (10 × 10 −9 m), which was then transferred in the laboratory freezer at −20 °C. After 2 h, the DNA modified AuNPs was taken out and thawed at room temperature.…”

Section: Preparation Of Probe a Modified Aunps And Gold Electrodementioning

confidence: 99%

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

Miao

Tang

2020

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AbstractmiRNAs, a class of endogenous noncoding RNAs, are involved in many crucial biological processes, which have emerged as a new set of biomarkers for disease theranostics. Exploring efficient signal amplification strategy is highly desired to pursue a highly sensitive miRNA biosensing platform. DNA nanotechnology shows great promise in the fabrication of amplified miRNA biosensors. In this work, a novel DNA walking and rolling nanomachine is developed for highly sensitive and selective detection of miRNA. Particularly, this approach programs two forms of dynamic DNA nanomachines powered by corresponding enzymes, which are well integrated. It is able to achieve a limit of detection as low as 39 × 10−18 m, along with excellent anti‐interfering performance and clinical applications. In addition, by designing pH‐controlled detachable intermolecular DNA triplex, the main sensing elements can be conveniently reset, which fulfills the requirements of point‐of‐care profiling of miRNA. The high consistency between the proposed approach and quantitative real‐time polymerase chain reaction validates the robustness and reliability. Therefore, it is anticipated that the DNA walking and rolling nanomachine has attractive application prospects in miRNA assay for biological researches and clinical diagnosis.

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Section: Preparation Of Probe a Modified Aunps And Gold Electrodementioning

confidence: 99%

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

Miao

Tang

2020

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“…Plasmonic gold‐based nanomaterials and their assemblies display powerful and tunable optical properties that have been widely investigated for bioapplications. [ 4,6–8 ] Based on Au nanoparticle (NP) probes, miRNA detection has been developed with optical sensing, [ 9 ] electrochemical detection methods, [ 10–12 ] fluorescence‐based detection, [ 13–16 ] and so on. [ 17 ] In recent years, NP‐based self‐assembling structures with enhanced chiroptical properties have been developed for the detection of several important biomarkers (DNA, RNA, proteins, etc.).…”

Section: Introductionmentioning

confidence: 99%

DNA‐Driven Two‐Layer Core–Satellite Gold Nanostructures for Ultrasensitive MicroRNA Detection in Living Cells

Meng

et al. 2020

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It is a significant challenge to achieve controllable self‐assembly of superstructures for biological applications in living cells. Here, a two‐layer core–satellite assembly is driven by a Y‐DNA, which is designed with three nucleotide chains that hybridized through complementary sequences. The two‐layer core–satellite nanostructure (C30S5S10 NS) is constructed using 30 nm gold nanoparticles (Au NPs) as the core, 5 nm Au NPs as the first satellite layer, and 10 nm Au NPs as the second satellite layer, resulting in a very strong circular dichroism (CD) and surface‐enhanced Raman scattering. After optimization, the yield is up to 85%, and produces a g‐factor of 0.16 × 10−2. The hybridization of the target microRNA (miRNA) with the molecular probe causes a significant drop in the CD and Raman signals, and this phenomenon is used to detect the miRNA in living cells. The CD signal has a good linear range of 0.011–20.94 amol ngRNA−1 and a limit of detection (LOD) of 0.0051 amol ngRNA−1, while Raman signal with the range of 0.052–34.98 amol ngRNA−1 and an LOD of 2.81 × 10−2 amol ngRNA−1. This innovative dual‐signal method can be used to quantify biomolecules in living cells, opening the way for ultrasensitive, highly accurate, and reliable diagnoses of clinical diseases.

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“…Redox SW peak P enabled us to measure RNA down to 1 nM concentration (Figure D 1 ), making it less sensitive than catalytic CPS peak H. CPS is thus better suited for sensitive measurements than SWVS or CV, because catalytic peaks obtained with CPS at low current densities arise from the exchange of many electrons per RNA molecule, as compared to 2–4 electron exchange during redox processes (using peak CA or peak P). Of course, sensitivity of our label‐free reagent‐less approach cannot compete with various label‐based assays for miRNA detection, often reaching LODs in femtomolar or even attomolar range . On the other hand, we have recently introduced electrochemical bioplatform for miR‐21 detection in clinical samples (we used samples from women suffering from cervical precancerous lesions), which was based on specific S9.6 antibody capturing miRNA‐DNA duplexes and hybridization chain reaction .…”

Section: Resultsmentioning

confidence: 99%

Intrinsic Electrocatalysis of RNA as a Label‐free and Reagent‐less Tool for Detection of MicroRNAs

2019

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We show for the first time that RNA catalyzes hydrogen evolution reaction at mercury‐containing electrodes. We previously showed that DNA is electrocatalytically active, and so we compared heights and potentials of the RNA chronopotentimetric stripping (CPS) peaks with analogous signals of DNA of the same sequence and found out they were very similar. RNA peaks showed differences depending on the RNA base composition. Catalytic nature of CPS peak enabled detection of 25 pM microRNA in electrochemical cell, or 500 pM microRNA in a 5 μL solution drop (corresponding to 2.5 fmole of microRNA). This finding opens the door for simple, label‐free and reagent‐less analysis of low concentrations of RNA molecules.

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Triple Signal Amplification Strategy for Ultrasensitive Determination of miRNA Based on Duplex Specific Nuclease and Bridge DNA–Gold Nanoparticles

Cited by 107 publications

References 41 publications

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

DNA Walking and Rolling Nanomachine for Electrochemical Detection of miRNA

DNA‐Driven Two‐Layer Core–Satellite Gold Nanostructures for Ultrasensitive MicroRNA Detection in Living Cells

Intrinsic Electrocatalysis of RNA as a Label‐free and Reagent‐less Tool for Detection of MicroRNAs

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