Triplet Excited States as a Source of Relevant (Bio)Chemical Information

Abstract

Triplet excited states exhibit strongly medium-dependent properties, which turns them into valuable tools to probe the microenvironments existing in protein binding Finally, protein encapsulation can also modify the photoreactivity of the guest. This is illustrated by presenting an example of retarded photooxidation. 2 Graphical abstractKeywords: 1-acid glycoproteins, drug, laser flash photolysis, serum albumins, protein binding, transient absorption spectroscopy.

Investigation of metabolite-protein interactions by transient absorption spectroscopy and in silico methods

“…The properties of triplet excited states result to be markedly medium-dependent; in this context, they can be excellent reporters to investigate compartmentalized microenvironments, including the protein binding pockets. [14]. We have previously shown that the behavior of triplet excited states of ligands within transport proteins (such as1-acid glycoproteins or serum albumins), monitored by transient absorption spectroscopy, constitutes a powerful tool to examine drug binding.…”