Tripterygium wilfordii protects against an animal model of autoimmune hepatitis

Zhang, Ting; Rao, Qianru; Dai, Manyun; Wu, Zhanxuan E.; Zhao, Qi; Li, Fēi

doi:10.1016/j.jep.2023.116365

Cited by 7 publications

(4 citation statements)

References 56 publications

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“…Five μL of the sample solution was added into the UPLC–ESI–QTOFMS system. In this study, the extraction and processing software and methods of the LC–MS raw data were carried out as previously described [ 51 ]. The principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA) were analyzed using SIMCA-P + 14.1 (Umetrics, San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Trizol reagent (Vazyme, Nanjing, China) was applied to distill mRNA from about 20 mg of duodenum, ileum, and liver. Quantitative real-time PCR (qPCR) was carried out as previously described [ 51 ]; the primer sequences are listed in Appendix A Table A1 . The mRNA levels of GAPDH were picked to normalize the target genes, and the thermal cycle conditions followed the scheme described previously [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

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Aspirin Caused Intestinal Damage through FXR and ET-1 Signaling Pathways

Lin,

Zhang,

Dai

et al. 2024

IJMS

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Aspirin is a non-steroidal, anti-inflammatory drug often used long term. However, long-term or large doses will cause gastrointestinal adverse reactions. To explore the mechanism of intestinal damage, we used non-targeted metabolomics; farnesoid X receptor (FXR) knockout mice, which were compared with wild-type mice; FXR agonists obeticholic acid (OCA) and chenodeoxycholic acid (CDCA); and endothelin-producing inhibitor estradiol to explore the mechanisms of acute and chronic intestinal injuries induced by aspirin from the perspective of molecular biology. Changes were found in the bile acids taurocholate acid (TCA) and tauro-β-muricholic acid (T-β-MCA) in the duodenum, and we detected a significant inhibition of FXR target genes. After additional administration of the FXR agonists OCA and CDCA, duodenal villus damage and inflammation were effectively improved. The results in the FXR knockout mice and wild-type mice showed that the overexpression of endothelin 1 (ET-1) was independent of FXR regulation after aspirin exposure, whereas CDCA was able to restore the activation of ET-1, which was induced by aspirin in wild-type mice in an FXR-dependent manner. The inhibition of ET-1 production could also effectively protect against small bowel damage. Therefore, the study revealed the key roles of the FXR and ET-1 pathways in acute and chronic aspirin-induced intestinal injuries, as well as strategies on alleviating aspirin-induced gastrointestinal injury by activating FXR and inhibiting ET-1 overexpression.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Aspirin Caused Intestinal Damage through FXR and ET-1 Signaling Pathways

Lin,

Zhang,

Dai

et al. 2024

IJMS

Self Cite

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“…(2) Known targets (e.g., HER2 for breast cancer [ 93 ]) and potential compounds in TCM or Chinese medicine compounds are screened. For example, nuclear receptor PXR is the target of autoimmune hepatitis [ 94 ], and PXR is used as a target for screening potential ligands from compound libraries or TCM. The general steps are as follows: disease targets are obtained through disease databases or experiments for receptor identification.…”

Section: Gpcrs Help In the Discovery Of New Targeted Tcm Or Drugsmentioning

confidence: 99%

G protein-coupled receptors and traditional Chinese medicine: new thinks for the development of traditional Chinese medicine

Zhang,

An,

You

et al. 2024

Chin Med

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G protein-coupled receptors (GPCRs) widely exist in vivo and participate in many physiological processes, thus emerging as important targets for drug development. Approximately 30% of the Food and Drug Administration (FDA)-approved drugs target GPCRs. To date, the ‘one disease, one target, one molecule’ strategy no longer meets the demands of drug development. Meanwhile, small-molecule drugs account for 60% of FDA-approved drugs. Traditional Chinese medicine (TCM) has garnered widespread attention for its unique theoretical system and treatment methods. TCM involves multiple components, targets and pathways. Centered on GPCRs and TCM, this paper discusses the similarities and differences between TCM and GPCRs from the perspectives of syndrome of TCM, the consistency of TCM’s multi-component and multi-target approaches and the potential of GPCRs and TCM in the development of novel drugs. A novel strategy, ‘simultaneous screening of drugs and targets’, was proposed and applied to the study of GPCRs. We combine GPCRs with TCM to facilitate the modernisation of TCM, provide valuable insights into the rational application of TCM and facilitate the research and development of novel drugs. This study offers theoretical support for the modernisation of TCM and introduces novel ideas for development of safe and effective drugs.

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“…Recent studies have focused on understanding the pharmacodynamics, pharmacokinetics, and toxicology of triptolide to reduce its toxicity and improve clinical efficacy ( Xu et al, 2016 ). Tripterygium and its extracts have been found effective in treating diabetic nephropathy by reducing urine protein and protecting renal function through anti-inflammatory, anti-oxidative, and anti-fibrotic mechanisms ( Liu et al, 2019 ; Zhang et al, 2023 ). Despite potential adverse effects, Tripterygium and its extracts are considered alternative medicines for diabetic nephropathy when used cautiously ( Zhang Y. et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Tripterygium drug-loaded liposome alleviates renal function by promoting vascularization and inhibiting fibrosis

Chen,

Wang,

Zhou

et al. 2024

Front. Chem.

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Introduction: Tripterygium species have been traditionally used in Chinese medicine for treating various conditions. The aim of the study was to construct a drug-modified renal infarction targeting liposome (rTor-LIP) containing Tripterygium in order to improve the therapeutic effect on renal injury.Methods: rTor-LIP was prepared using the extruder method containing Tripterygium solution. The preparation was characterized by transmission electron microscopy, Marvin laser particle size analyzer, and Western blotting. In vitro experiments were conducted to verify the biocompatibility of rTor-LIP, and in vivo experiments were conducted to verify the therapeutic effect of rTor- LIP on renal injury.Results and discussion: The surface of rTor-LIP was regular and oval. In vitro results showed that after co-incubation with rTor-LIP, endothelial cells did not show significant apoptosis, and there were no significant abnormalities in the mitochondrial metabolism. The in vivo results showed that the morphology of endothelial cells in the rTor-LIP group was uniform and the cytoplasmic striations were clear, but the local striations had disappeared. Thus, rTor-LIP nano-targeted liposomes can effectively target hypoxic kidney tissue, providing a new idea for the treatment of renal infarction.

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Tripterygium wilfordii protects against an animal model of autoimmune hepatitis

Cited by 7 publications

References 56 publications

Aspirin Caused Intestinal Damage through FXR and ET-1 Signaling Pathways

Aspirin Caused Intestinal Damage through FXR and ET-1 Signaling Pathways

G protein-coupled receptors and traditional Chinese medicine: new thinks for the development of traditional Chinese medicine

Tripterygium drug-loaded liposome alleviates renal function by promoting vascularization and inhibiting fibrosis

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