Triptolide Suppresses Alkali Burn‐Induced Corneal Angiogenesis Along with a Downregulation of VEGFA and VEGFC Expression

Geng, Wenjing; Li, Na; Lv, Xiaoyi; Ahmed, Naila; Li, Xinlei; Liu, Huidong; Ma, Jing; Zhang, Yafang

doi:10.1002/ar.23583

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…Our results showed that the expression of PCNA in blood increased, but in skin decreased. Different from our results, another study reported increased expression of VEGF in the alkali burn-induced corneal angiogenesis mouse model ( 33 ). The decrease of VEGFA expression would induce the inhibition of angiogenesis ( 34 ).…”

Section: Discussioncontrasting

confidence: 99%

Molecular imbalance mechanism of skin and blood leucocytes in severe burn patients at different burn times

Liang¹,

Qiu²,

Wu³

et al. 2022

Ann Transl Med

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Background: Severe burns are a leading cause of injuries worldwide and are usually accompanied by considerable morbidity and mortality. The purpose of this study was to investigate the changes of gene expression in blood and skin at different times after severe burn.Methods: Firstly, the gene expression profiles of different burn time samples in GSE19743 and GSE8056 were analyzed. Secondly, the maladjusted gene network was identified by protein-protein interaction (PPI) network, and the genes in the network were enriched and analyzed. In addition, the key dysfunctional genes were identified by betweenness algorithm, and evaluated by survival analysis, Cox analysis, receiver operating characteristic (ROC) analysis. Finally, crosstalk analysis and enrichment analysis were carried out between the blood-and skin-specific differentially expressed genes (DEGs) at different burn times. Results:The results showed that there were common DEGs in the blood and skin at different burn times.Importantly, we screened out the key dysfunctional genes BIRC5, NCAM1, PCNA, TOP2A, and VEGFA, which were related to the course of burns. Enrichment analysis showed that these maladjusted genes were mainly involved in the immune inflammation-related signal pathway. Additionally, significant crosstalk was identified between blood-and skin-specific genes at different burn times, especially in the blood. The signal pathways involved in specific genes represent their own pathological characteristics.Conclusions: Both blood and skin tissues express common pathological changes and unique molecular mechanisms at different times after burn injury. The results of this study provide guidance for clinical personalized treatment.

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Section: Discussioncontrasting

confidence: 99%

Molecular imbalance mechanism of skin and blood leucocytes in severe burn patients at different burn times

Liang¹,

Qiu²,

Wu³

et al. 2022

Ann Transl Med

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“…Membranes were blocked with a buffer containing Tris (10 mM, pH 7.4), NaCl (150 mM), Tween 20 (0.1%), and BSA (5%) and then incubated with the primary antibodies at a dilution of 1:1,000 at 4°C overnight. Subsequently, membranes were washed in TBST for 15 min twice and incubated with appropriate secondary antibodies for 2 h at room temperature (Wang et al, ). Bands and signals were collected by G: BOX Chemi XT4 (Syngene, UK).…”

Section: Methodsmentioning

confidence: 99%

Myricetin Induces Protective Autophagy by Inhibiting the Phosphorylation of mTOR in HepG2 Cells

Cao

Chen

et al. 2017

The Anatomical Record

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Myricetin, a natural flavonoid present in a variety of fruits and vegetables, has been studied as a promising cancer chemopreventive agent in many cancer models. It has been reported that myricetin could inhibit tumor promotion by inducing cell cycle arrest and promoting apoptotic cell death. At present, autophagy is considered to be closely associated with cancer, functioning as either an anti-cancer or pro-cancer mechanism depending on the cancer stage. Till date, the role of myricetin in regulating autophagy has not been reported. In this study, we found that myricetin can induce autophagy by inhibiting mTOR activation in HepG2 cells. Our findings thus provide evidence for further research and application of myricetin as a potential cancer therapeutic agent. Anat Rec, 301:786-795, 2018. © 2017 Wiley Periodicals, Inc.

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“…To evaluate its effect on angiogenesis, it was tested in vitro and in vivo. Triptolide inhibited rat aortic endothelial cell migration and tube formation and significantly suppressed CoNV and VEGF expression in a murine alkali burn model [126]. While these results indicate that triptolide may have clinical indications for the treatment of CoNV, further studies are needed to assess its exact mechanism of action, optimal dose, and potential toxic side effects with long term use.…”

Section: Non-flavonoid Phytochemicalsmentioning

confidence: 94%

Pharmacological Potential of Small Molecules for Treating Corneal Neovascularization

2020

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Under healthy conditions, the cornea is an avascular structure which allows for transparency and optimal visual acuity. Its avascular nature is maintained by a balance of proangiogenic and antiangiogenic factors. An imbalance of these factors can result in abnormal blood vessel proliferation into the cornea. This corneal neovascularization (CoNV) can stem from a variety of insults including hypoxia and ocular surface inflammation caused by trauma, infection, chemical burns, and immunological diseases. CoNV threatens corneal transparency, resulting in permanent vision loss. Mainstay treatments of CoNV have partial efficacy and associated side effects, revealing the need for novel treatments. Numerous natural products and synthetic small molecules have shown potential in preclinical studies in vivo as antiangiogenic therapies for CoNV. Such small molecules include synthetic inhibitors of the vascular endothelial growth factor (VEGF) receptor and other tyrosine kinases, plus repurposed antimicrobials, as well as natural source-derived flavonoid and non-flavonoid phytochemicals, immunosuppressants, vitamins, and histone deacetylase inhibitors. They induce antiangiogenic and anti-inflammatory effects through inhibition of VEGF, NF-κB, and other growth factor receptor pathways. Here, we review the potential of small molecules, both synthetics and natural products, targeting these and other molecular mechanisms, as antiangiogenic agents in the treatment of CoNV.

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Triptolide Suppresses Alkali Burn‐Induced Corneal Angiogenesis Along with a Downregulation of VEGFA and VEGFC Expression

Cited by 8 publications

References 29 publications

Molecular imbalance mechanism of skin and blood leucocytes in severe burn patients at different burn times

Molecular imbalance mechanism of skin and blood leucocytes in severe burn patients at different burn times

Myricetin Induces Protective Autophagy by Inhibiting the Phosphorylation of mTOR in HepG2 Cells

Pharmacological Potential of Small Molecules for Treating Corneal Neovascularization

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