Tris(2-carboxyethyl)phosphine-Mediated Nanometric Extracellular Matrix-Coating Method of Mesenchymal Stem Cells

Koh, Ilkyoo; Yong, Insung; Kim, Bumsoo; Choi, Daheui; Hong, Jinkee; Han, Yong Mahn; Kim, Pilnam

doi:10.1021/acsbiomaterials.9b01480

Cited by 6 publications

(11 citation statements)

References 46 publications

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Section: Demonstrated Applicationsmentioning

confidence: 99%

“…[191,192] Such drawbacks can be overcome by coating individual cells with ECM-mimetic materials prior to spheroid formation. For example, MSCs, derived from human iPSCs, were coated with collagen type I or decellularized bone ECMs (Figure 10b), [193] after mild TCEP treatment to expose free thiols at the membranes, which apparently enhanced the adsorption of ECM components onto the cell surfaces. [194] MSC@ECM showed the enhanced viability after the formation [193] Copyright 2020, The American Chemical Society.…”

Section: Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

“…For example, MSCs, derived from human iPSCs, were coated with collagen type I or decellularized bone ECMs (Figure 10b), [193] after mild TCEP treatment to expose free thiols at the membranes, which apparently enhanced the adsorption of ECM components onto the cell surfaces. [194] MSC@ECM showed the enhanced viability after the formation [193] Copyright 2020, The American Chemical Society. c) (Left) Schematic for formation of GLN-hydrogel shells on individual MSCs.…”

Section: Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

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Bioempowerment of Therapeutic Living Cells by Single‐Cell Surface Engineering

Yang

Choi

Nguyen

et al. 2023

Advanced Therapeutics

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Living cells have the irreplaceable capability to achieve a wide range of complex biochemical reactions precisely and efficiently, which makes them attractive materials for therapeutic applications. In lieu of the traditional biochemical and biological approaches primarily focused on the augmentation of the innate functions of cells, there has been appreciable progress in the development of engineered therapeutic cells, mainly based on the chemical modifications of cell surfaces, at the single‐cell level, which empowers individual living cells with designed therapeutic functions in a cytocompatible manner. This review highlights the latest advances in the development of therapeutic living cells using single‐cell surface engineering, for potential applications in blood transfusion, drug delivery, cancer therapy, probiotic therapy, and tissue engineering and regenerative medicine. The methodological strategies for functionalizing cell surfaces with biomolecules, and inorganic and organic materials, to endow living cells with extrinsic physicochemical and biological properties as well as to increase the durability and efficacy of engineered therapeutic cells, are also briefly overviewed. The review ends with a perspective that discusses the construction of active cell‐in‐shell nanobiohybrid systems, in which exogenous materials formed on cell surfaces mutually and intimately communicate with the cells inside, as a future research direction for single‐cell surface engineering.

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Section: Demonstrated Applicationsmentioning

confidence: 99%

Section: Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Section: Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

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Bioempowerment of Therapeutic Living Cells by Single‐Cell Surface Engineering

Yang

Choi

Nguyen

et al. 2023

Advanced Therapeutics

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“…Kim and co‐workers found that the TCEP treatment on U87 and U251 glioma cells promoted the cellular anchorage to the ECM, thus enhancing cell adhesion. [ 79,80 ] The TCEP treatment also increased the expression level of focal adhesion complex‐related proteins, such as vinculin and an activated form of FAK. Due to these advantages, the coating efficiency of decellularized bone ECM materials for individual human‐induced pluripotent stem cells (iPSCs) was significantly enhanced, as well as for 3D cell spheroids.…”

Section: Materials and Strategies For Mammalian‐cell Nanocoatingmentioning

confidence: 99%

A Decade of Advances in Single‐Cell Nanocoating for Mammalian Cells

Lee

Kim

Rheem

et al. 2021

Adv Healthcare Materials

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Strategic advances in the single‐cell nanocoating of mammalian cells have noticeably been made during the last decade, and many potential applications have been demonstrated. Various cell‐coating strategies have been proposed via adaptation of reported methods in the surface sciences and/or materials identification that ensure the sustainability of labile mammalian cells during chemical manipulation. Here an overview of the methodological development and potential applications to the healthcare sector in the nanocoating of mammalian cells made during the last decade is provided. The materials used for the nanocoating are categorized into polymers, hydrogels, polyphenolic compounds, nanoparticles, and minerals, and the corresponding strategies are described under the given set of materials. It also suggests, as a future direction, the creation of the cytospace system that is hierarchically composed of the physically separated but mutually interacting cellular hybrids.

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“…However, despite these merits, some limitations exist for its clinical application. On one hand, TCEP has safety concerns, such as toxicity at concentrations over 1 × 10 –2 M, making it unsuitable for the direct administration on organisms. , On the other hand, TCEP disrupts mucus strands, thus inhibiting the mucociliary transport of large particles . Therefore, the development of alternative biocompatible and effective mucolytic agents is of great significance in the treatment of airway obstruction diseases.…”

Section: Introductionmentioning

confidence: 99%

Mucoadhesive, Antibacterial, and Reductive Nanogels as a Mucolytic Agent for Efficient Nebulized Therapy to Combat Allergic Asthma

Zhao

Cheng

et al. 2022

ACS Nano

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Asthma is an intractable disease involving the infiltration of inflammatory cells and mucus plugging. Despite small molecular mucolytics having the ability to break the disulfide bonds of mucins, offering a potential way to overcome the airflow obstruction and airway infection, these mucolytics have limited therapeutic effects in vivo. Therefore, in this work, arginine-grafted chitosan (CS-Arg) is ionically cross-linked with tris(2-carboxyethyl)phosphine (TCEP) to obtain nanogels as a mucolytic agent. The positively charged nanogels effectively inhibit the formation of large aggregates of mucin in vitro, probably thanks to the formation of an ionic interaction between CS-Arg and mucin, as well as the breakage of disulfide bonds in mucin by the reductive TCEP. Moreover, the nanogels show good cytocompatibility at concentrations up to 5 mg mL −1 , exhibiting effective inhibitory effects against the proliferation of both Staphylococcus aureus and Escherichia coli at 5 mg mL −1 . After the administration of the nanogels by nebulization into a Balb/c mouse model with allergic asthma, they can efficiently reduce the mucus obstruction in bronchioles and alveoli and relieve airway inflammation. Therefore, these CS-Arg/TCEP nanogels potentially represent a promising mucolytic agent for the efficient treatment of allergic asthma and other muco-obstructive diseases.

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Tris(2-carboxyethyl)phosphine-Mediated Nanometric Extracellular Matrix-Coating Method of Mesenchymal Stem Cells

Cited by 6 publications

References 46 publications

Bioempowerment of Therapeutic Living Cells by Single‐Cell Surface Engineering

Bioempowerment of Therapeutic Living Cells by Single‐Cell Surface Engineering

A Decade of Advances in Single‐Cell Nanocoating for Mammalian Cells

Mucoadhesive, Antibacterial, and Reductive Nanogels as a Mucolytic Agent for Efficient Nebulized Therapy to Combat Allergic Asthma

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