“…However, in most cases, the studies focus on identifying and/or quantification of terpenes (TAKETA et al, 2000 and2004), saponins (GNOATTO et al, 2005;PIRES et al, 1997;REGINATTO et al, 1999), polyphenols (BRAVO et al, 2007), methylxanthines (FILIP et al, 1998and 2010, REGINATTO et al, 1999 and flavonoids (TARRAGO et al, 2005, FILIP et al, 2010. In a previous study, Pires et al (1997) observed differences in the chemical composition of two species of Ilex (dumosa and paraguariensis), especially related to the concentration of saponins.…”