Triterpenes From Docynia Indica Fruits and Their Cytotoxic Activity

Abstract

Five triterpenes were isolated from the ethyl acetate fraction of methanolic extract of Docynia indica fruits. Their structures were determined to be pomolic acid (1), maslinic acid (2), ursolic acid (3), 23-hydroxy ursolic acid (4) and euscaphic acid (5) by comparison of physicochemical, NMR and mass spectral data with those reported in the literature. All triterpenes (1 – 5) were isolated for the first time from Docynia indica. Compounds 1 – 5 were evaluated for their cytotoxic activity using MTT assay, of which 23-hydroxy ursolic acid (3) and ursolic acid (4) showed cytotoxic activities against HeLa and Hep-G2 cancer cell lines.

In Silico Assessment and Molecular Docking Studies of Some Phyto-Triterpenoid for Potential Disruption of Mortalin-p53 Interaction

“…The chemical structures of 41 phyto triterpenes were collected from published literatures for study [1,[3][4][5][6] (Figure S1). To establish the chemical structures of the triterpenes, Marvin software was used.…”

“…Thus, any ligand whose docking energy falls within this range or more negative would be considered as a potential inhibitor of Mortalin. It is indicated from Table 4 The cytotoxic activity of the tested compounds on HepG2 from literature was retrieved to gain better insight into the effects resulting from the disruption of moratlin-p53 interaction caused by potential compounds (Table 4) [1,[3][4][5][6][7][8].…”

“…The disease is also known for its high recurrence rate in the post-treatment period, urging for an accurate prediction of the early risk in postoperative patients. Among various mechanisms through which HCC develops, mortalin, a stress chaperone of Hsp70 family of proteins, was shown to have a close link with liver cancer incidence through the apprehension of the p53 tumor suppressor protein [3][4][5]. Though the complexity of p53 function in tumor suppression has been investigated recently in which it regulates both pro-oxidant and antioxidant target genes for complete opposite effects [6].…”