Triterpenoid content and expression of triterpenoid biosynthetic genes in birch (Betula platyphylla Suk) treated with 5-azacytidine

Zeng, Fansuo; Li, Xiaohua; Qie, Rui; Li, Leilei; Ma, Minghao; Yu, Zhan

doi:10.1007/s11676-019-00966-1

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…For example, in the study of Catharanthus roseus cell suspension cultures, the concertation of 5-Az was used about 40.95 to 81.9 μM for the formation of secondary metabolites 47 . On the other hand, triterpenoid contents and biosynthetic genes in Betula platyphylla were induced by 5-Az with 50–500 μM 49 . In addition, 200 μM 5-Az significantly increased the resveratrol production up to 2.0-fold in Vitis amurensis 48 .…”

Section: Discussionmentioning

confidence: 99%

“…5-Az could induce various plant phenotype changes, including dwarfism, early flowering and inhibition of vegetative growth 46 . In addition, the stimulating effect of DNA methyltransferase inhibitors on the accumulation of secondary metabolites has also been investigated in cell suspension cultures of Catharanthus roseus , Betula platyphylla and Vitis amurensis 47 – 49 .…”

Section: Introductionmentioning

confidence: 99%

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5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation

Yang

Lee

Lin

et al. 2022

Sci Rep

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Recent studies have indicated strong connections between epigenetic modulation and secondary metabolites in plants. It is vital to understand the roles of epigenetics in the production of secondary metabolites. In this study, the inhibitor of DNA methylation 5-azacytidine (5-Az) was used on the hairy roots of the medicinal plant Salvia miltiorrhiza to investigate its effect on secondary metabolite production, gene expression, methylation levels in genomic DNA and promoter regions. Our results showed that the contents of tanshinones in S. miltiorrhiza hairy roots increased by 1.5–5 times, and some genes in the biosynthesis pathway showed an upward trend. According to our NGS analysis, the methylation pattern in the promotor of the gene encoding copalyl diphosphate synthase (CPS) was altered, and 51 out of 145 cytosines were demethylated during 5-Az treatment. A total of 36 putative transcription factors (TFs) binding cites were identified in these demethylation sites. Among these TFs binding cites, cis-regulatory elements for the binding of NF-Y and MYB were frequently found in our results. This is the first report to demonstrate a possible mechanism of DNA methylation participating in tanshinone biosynthesis in S. miltiorrhiza hairy roots by modulating the CPS promoter and TFs binding sites.

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Section: Discussionmentioning

confidence: 99%