Triterpenoid glycosides from the rhizomes of Allium ascalonicum and their anoctamin-1 inhibitory activity

Abstract

Ten triterpenoid glycosides including two new (1 and 2) were isolated from the methanol extract of Allium ascalonicum rhizomes. These compounds were structurally elucidated to be 3β(2), lactifoloside C (3), lactifoloside H (4), randiasaponin IV (5), kudinoside G (6), ilexkudinoside W (7), lactifoloside G (8), kudinoside D (9), and ilexkudinoside T (10) by analyzing their HR-ESI-MS, NMR spectral data and by comparison with those reported in the literature. Compounds 1-10 were evaluated for anoctamin-1 (ANO1) inhibitory activity using yellow fluorescent protein reduction assays. At the concentration of 30 µM, compounds 2 and 9 displayed significant ANO1 inhibitory percentages of 28.9 and 26.2%, respectively.

Therapeutic Uses and Pharmacological Properties of Shallot (Allium ascalonicum): A Systematic Review

“…Mai et al isolated ten triterpenoid glycosides from the methanol extract of A. ascalonicum rhizomes, which were evaluated for anoctamin-1 (ANO1) inhibitory activity using yellow fluorescent protein reduction assays. The study revealed that among them, two compounds (compounds 2 and 9, Figure 3G ) showed moderate ANO1 inhibitory percentages ( 83 ).…”

“…(G) Chemical structures of compounds 1–10 isolated from shallot screened for anoctamin inhibitory capacity. Compounds 2 and 9 exerted the highest anoctamin inhibitory potential ( 78 – 83 ).…”