Triterpenoid saponins from the roots of<i>Acanthophyllum gypsophiloides</i>Regel

Khatuntseva, Elena A.; Men’shov, V. M.; Shashkov, Alexander S.; Tsvetkov, Yury E.; Stepanenko, R. N.; Vlasenko, R. Ya.; Шульц, Э. Э.; Толстиков, Г. А.; Толстикова, Т. Г.; Baev, Dmitry S.; Kaledin, Vasiliy A; Popova, Nelli A; Николин, В. П.; Laktionov, Pavel P.; Cherepanova, Anna V.; Kulakovskaya, T. V.; Kulakovskaya, Ekaterina; Nifantiev, Nikolay E.

doi:10.3762/bjoc.8.87

Cited by 21 publications

(6 citation statements)

References 29 publications

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“…Therefore, in this study, we assessed its in vitro hemolytic activity against mice erythrocytes to determine a safe dose for use in our vaccine formulations. A published protocol was followed [ 43 ] with few modifications. Briefly, 2 mL of blood from two adult CO 2 -euthanized mice was collected via cardiac puncture into a 15 mL centrifuge tube containing 80 µL, 6.32 USP units per mL, of BD sodium heparin and mixed gently with 5 mL PBS.…”

Section: Methodsmentioning

confidence: 99%

Safe Subunit Green Vaccines Confer Robust Immunity and Protection against Mucosal Brucella Infection in Mice

et al. 2023

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Brucellosis is a zoonotic disease that causes significant negative impacts on the animal industry and affects over half a million people worldwide every year. The limited safety and efficacy of current animal brucellosis vaccines, combined with the lack of a licensed human brucellosis vaccine, have led researchers to search for new vaccine strategies to combat the disease. To this end, the present research aimed to evaluate the safety and efficacy of a green vaccine candidate that combines Brucella abortus S19 smooth lipopolysaccharide (sLPS) with Quillaja saponin (QS) or QS-Xyloglucan mix (QS-X) against mucosal brucellosis in BALB/C mice. The results of the study indicate that administering two doses of either sLPS-QS or sLPS-QS-X was safe for the animals, triggered a robust immune response, and enhanced protection following intranasal challenge with S19. Specifically, the vaccine combinations led to the secretion of IgA and IgG1 in the BALF of the immunized mice. We also found a mixed IgG1/IgG2a systemic response indicating evidence of both Th1 and Th2 activation, with a predominance of the IgG1 over the IgG2a. These candidates resulted in significant reductions in the bioburden of lung, liver, and spleen tissue compared to the PBS control group. The sLPS-QS vaccination had conferred the greatest protection, with a 130-fold reduction in Brucella burdens in lung and a 55.74-fold reduction in the spleen compared to PBS controls. Vaccination with sLPS-QS-X resulted in the highest reduction in splenic Brucella loads, with a 364.6-fold decrease in bacterial titer compared to non-vaccinated animals. The study suggests that the tested vaccine candidates are safe and effective in increasing the animals’ ability to respond to brucellosis via mucosal challenge. It also supports the use of the S19 challenge strain as a safe and cost-effective method for testing Brucella vaccine candidates under BSL-2 containment conditions.

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Section: Methodsmentioning

confidence: 99%

Safe Subunit Green Vaccines Confer Robust Immunity and Protection against Mucosal Brucella Infection in Mice

et al. 2023

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“…Pulsatilla chinensis, another traditional Chinese medicine rich in Pentacyclic triterpenoid saponin (PTSs), and saponins may be the main components of its antibacterial activity (60). Sugars are attached to one or more points of this structure, forming chains that could be branched.…”

Section: Vulvovaginal Candidiasismentioning

confidence: 99%

The effect of herbal medicine in innate immunity to Candida albicans

Bao

et al. 2023

Front. Immunol.

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Candida albicans (C. albicans) is an opportunistic pathogenic fungus that often causes mucosal and systemic infections. Several pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs) and C-type lectin receptors (CLRs), have been implicated in the host recognition of C. albicans. These PRRs recognize the pathogen-associated molecular patterns (PAMPs) of C. albicans to activate innate immune cells, thereby rapidly inducing various inflammatory responses by activating intracellular signaling cascades. Herbal medicine and its active components deserve priority development due to their low toxicity and high antibacterial, antiviral and antifungal activities. This review discussed the activities of herbal compounds against C. albicans and their related mechanisms, especially their regulatory role on innate immune cells such as neutrophils, macrophages, and dendritic cells (DCs) implicated in C. albicans infections. Our work aims to find new therapeutic drugs and targets to prevent and treat diseases caused by C. albicans infection with the mechanisms by which this fungus interacts with the innate immune response.

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“…The rare TSR species has pharmacological value as an expectorant, diuretic and a laxative natural remedy and technical application as an effective foaming agent and emulsifier [3]. TSR contains a complex of triterpene saponins of the oleanolic series, from which saponins-derivatives of gypsogenin and quillic acid-have been isolated [4,5]. They exhibit immunostimulant and antiviral properties [6].…”

Section: Introductionmentioning

confidence: 99%

Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of Allochrusa gypsophiloides (Regel) Schischk Compared to Wild Plants

Mursaliyeva,

Sarsenbek,

Dzhakibaeva

et al. 2023

Plants

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Allochrusa gypsophiloides is a rare Central Asian species, a super-producer of triterpene saponins with pharmacological and technical value. In this work, a comparative evaluation of the in vitro culture of adventitious roots (ARs), in vitro adventitious microshoots (ASs), natural roots and aboveground parts of wild plants from Kazakhstan to define the total saponin (TS), phenol (TP) and flavonoid (TF) content, as well as antioxidant (AOA) and antimicrobial activity, is presented for the first time. In the AR culture, growth index (GI), TS, TP and TF were evaluated on days 25, 45 and 60 of cultivation on ½ MS medium without (control) and with auxin application. It was found out that TS and TF were higher in the in vitro AR culture. The amount of TP and TF are higher in the aerial part of vegetative plants with maximum AOA. The concentration of the extract required to inhibit 50% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical formation (ICO50) in extracts from natural material negatively correlated with TS, TP, TF and in the in vitro AR culture with TF. Control extracts from the in vitro AR culture with high TS levels showed growth-inhibitory activity against S. thermophillus, S. cerevisiae and C. albicans. The influence shares of medium composition factor, cultivation duration factor and their interaction with GI, TS, TP and TF were determined. The in vitro AR culture is promising for obtaining triterpene saponins TSR with high antibacterial and antifungal activity, and the in vitro ASs culture—for shoot multiplication with antioxidant properties.

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Triterpenoid saponins from the roots ofAcanthophyllum gypsophiloidesRegel

Cited by 21 publications

References 29 publications

Safe Subunit Green Vaccines Confer Robust Immunity and Protection against Mucosal Brucella Infection in Mice

Safe Subunit Green Vaccines Confer Robust Immunity and Protection against Mucosal Brucella Infection in Mice

The effect of herbal medicine in innate immunity to Candida albicans

Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of Allochrusa gypsophiloides (Regel) Schischk Compared to Wild Plants

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