The essential oils from an alpine needle leaf of Abies koreana, which is a species native to Korea, was obtained by hydrodistillation with 0.9% (v/w) yield and analysed by GC and GC-MS. Sixty-eight constituents, accounting for more than 95.9% of the total oil composition, were identified. The main compounds with concentrations higher than 3% were borneol (27.9%), alpha-pinene (23.2%), beta-pinene (5.8%), terpinene-4-ol (3.8%), bornyl acetate (3.4%) and alpha-terpineol (3.1%). The antibacterial activity of the oil was evaluated against nine microorganisms using disc diffusion and broth microdilution methods. The essential oil exhibited some broad spectrum antibacterial activity against the tested organisms, including methicillin-resistant Staphylococcus aureus.