Tritiated water exposure disrupts myofibril structure and induces mis-regulation of eye opacity and DNA repair genes in zebrafish early life stages

Arcanjo, Caroline; Armant, Olivier; Floriani, Magali; Cavalié, Isabelle; Camilleri, Virginie; Simon, Olivier; Orjollet, Daniel; Adam-Guillermin, Christelle; Gagnaire, Béatrice

doi:10.1016/j.aquatox.2018.04.012

Cited by 25 publications

(26 citation statements)

References 54 publications

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“…Results did not show significant differences between control and contaminated organisms for both dose rates. These results suggested that genes coding for proteins involved in muscle contraction found, via a transcriptomic analysis, to be affected in 24 hpf eggs for both dose rates (Arcanjo et al, 2018) did not induce any effect on spontaneous tail movement at the same developmental stage. The spontaneous movements of the tail originate from the spinal cord and are dependant of functional motoneuron innervation (Brustein et al, 2003) as well as muscle contraction.…”

Section: Hto Exposure Did Not Change Spontaneous Tail Movementmentioning

confidence: 82%

“…Furthermore, zebrafish is an emerging model for studying behavior and many tools are now available to assess plenty of behavioral parameters in adult and embryolarvae of zebrafish (Ahmad et al, 2012). In this project, a previous study was conducted and focused on the effects of HTO exposure on zebrafish early life stages at the molecular scale using a transcriptomic analysis by mRNAseq (Arcanjo et al, 2018). This study revealed an effect of HTO exposure on the expression of genes involved in muscle contraction, eye transparency, circadian clock, response to reactive oxygen species and repair of DNA damages.…”

Section: Introductionmentioning

confidence: 99%

“…In term of activity concentrations, these two doses rates corresponded to tritiated water activities of 1.22x10 5 and 1.22x10 6 Bq/mL, respectively. As the first part of the study highlighted differential expression of genes involved in muscle contraction (skeletal and cardiac) (Arcanjo et al, 2018), 24 hpf embryos to 96 hpf larvae were challenged with behavioral analyses including spontaneous tail movements, heart rate and free-swimming behavior. Histological observations of eyes from 48 hpf to 96 hpf were also performed to make the link with modulation of genes involved in eye transparency.…”

Section: Introductionmentioning

confidence: 99%

“…Finally, a whole mount immunostaining was performed to detect gamma-H2AX foci, an early marker of double strand break repair process (Rogakou et al, 1998), as some genes involved in DNA repair pathways were found mis-regulated in a previous study (Arcanjo et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

et al. 2020

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Section: Hto Exposure Did Not Change Spontaneous Tail Movementmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

et al. 2020

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“…It can bind to DNA damage-binding protein 1 (DDB1) to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). It has been reported that tritiated water exposure disrupts myofibril structure and induces misregulation of eye opacity through the mutation of CRYBA4 and DNA repair genes in early zebrafish life stages [43]. However, more experiments are needed to fully confirm the connection between CRYBA4 protein and DDB2 protein.…”

Section: G171s Xtalbgmentioning

confidence: 99%

In silico analysis and high-risk pathogenic phenotype predictions of non-synonymous single nucleotide polymorphisms in human Crystallin beta A4 gene associated with congenital cataract

Wang

Huang

et al. 2020

PLoS ONE

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In order to provide a cost-effective method to narrow down the number of pathogenic Crystallin beta A4 (CRYBA4) non-synonymous single nucleotide polymorphisms (nsSNPs), we collected nsSNP information of the CRYBA4 gene from SNP databases and literature, predicting the pathogenicity and possible changes of protein properties and structures using multiple bioinformatics tools. The nsSNP data of the CRYBA4 gene were collected from 4 databases and published literature. According to 12 criteria, six bioinformatics tools were chosen to predict the pathogenicity. I-Mutant 2.0, Mupro and INPS online tools were used to analyze the effects of amino acid substitution on protein stability by calculating the value of ΔΔG. ConSurf, SOPMA, GETAREA and HOPE online tools were used to predict the evolutionary conservation of amino acids, solvent accessible surface areas, and the physical and chemical properties and changes of protein structure. All 157 CRYBA4 nsSNPs were analyzed. Forty-four CRYBA4 high-risk pathogenic nsSNPs (predicted to be pathogenic by all six software tools) were detected out of the 157 CRYBA4 nsSNPs, four of which (c.283C>T, p.R95W; c.449T>A, p.V150D; c.475G>A, p.G159R; c.575G>C, p.R192P) should be focused on because of their high potential pathogenicity and possibility of changing protein properties. Thirty high-risk nsSNPs were predicted to cause a decrease of protein stability. Twenty-nine high-risk nsSNPs occurred in evolutionary conserved positions. Twenty-two high-risk nsSNPs occurred in the core of the protein. It is predicted that these high-risk pathogenic nsSNPs can cause changes in the physical and chemical properties of amino acids, resulting in structural changes of proteins and changes in the interactions between domains and other molecules, thus affecting the function of proteins. This study provides important reference value when narrowing down the number of pathogenic CRYBA4 nsSNPs and studying the pathogenesis of congenital cataracts. By using this method, we can easily find 44 high-risk pathogenic nsSNPs out of 157 CRYBA4 nsSNPs.

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Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

Gagnaire

Arcanjo

Cavalié

et al. 2020

Enviro Toxic and Chemistry

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Tritium, a radioactive isotope of hydrogen of natural and anthropogenic origin, is ubiquitously present in the environment. Effluents of nuclear centers of production are significant anthropogenic sources. With the upcoming project of thermonuclear fusion, tritium releases in the environment may increase. It is therefore important to characterize the ecological risk linked to tritium. The effects of tritiated water (HTO) were therefore studied in zebrafish larvae exposed for 10 d to different dose rates, 1.1 × 102, 4.1 × 102, and 3.8 × 103 µGy/h for larvae corresponding, respectively, to a water contamination of 104, 105, and 106 Bq/mL of HTO. Those dose rates were higher than 10 μGy/h, which is the threshold recommended to start monitoring ecosystems where radiological contaminants are present. Mortality, embryo‐larval development, immune toxicity, genotoxicity, neurotoxicity, and alterations of tissues were investigated. The results showed that HTO exposure induced DNA damage and reactive oxygen species production and modulated the expression of genes involved in detoxification processes. Moreover, modifications of the muscular tissues (degradation of myofibrils at 4 d post fertilization and disorganization of mitochondria at later stages) were observed. The results differed with HTO dose rates and with developmental stages. These results will drive future research for the development of new HTO‐sensitive biomarkers and will allow us to progress in the characterization of the modes of action of tritium in fish. Environ Toxicol Chem 2020;39:648–658. © 2019 SETAC

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Tritiated water exposure disrupts myofibril structure and induces mis-regulation of eye opacity and DNA repair genes in zebrafish early life stages

Cited by 25 publications

References 54 publications

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

Effects of tritiated water on locomotion of zebrafish larvae: a new insight in tritium toxic effects on a vertebrate model species

In silico analysis and high-risk pathogenic phenotype predictions of non-synonymous single nucleotide polymorphisms in human Crystallin beta A4 gene associated with congenital cataract

Tritiated Water Exposure in Zebrafish (Danio rerio): Effects on the Early‐Life Stages

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