RFID is a system that uses the radio waves to scrutinize and capture data pertained to a tag for an object attached to it. In spite of RFID's wide application in industries, it poses a severe security issue. There is high susceptibility that RFID might be attacked with future attacks to invade the privacy and data in the system. To protect the RFID system against such attacks, the Pad-generation (Pad-Gen) function is used. This paper presents a mutual authentication scheme Tag Reader Mutual Authentication (TRMA) that is implemented using two approaches, the XOR operation and the MOD operation by modifying the Pad-Gen function. The proposed framework is executed on low-cost Artix7 FPGA XC7A100T-3CSG324, and its hardware verification is done on chip scope pro tool.