Troglitazone Improves Insulin-Stimulated Glucose Utilization Associated with an Increased Muscle Glycogen Content in Obese Zucker Rats.

Oshida, Yoshiharu; Kako, Mami; Nakai, Norihiro; Shimomura, Yoshiharu; Li, Ling; Sato, Juichi; Ohsawa, Isao; Sato, Yuzo

doi:10.1507/endocrj.46.723

Cited by 15 publications

(12 citation statements)

References 37 publications

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“…There is considerable interest in regulating PDK4 as an approach to the treatment of type 2 diabetes Table 1. (Oshida et al 1999, Sreenan et al 1999, Sugden & Holness 2002. The last transcript in this group is HMG-CoA synthase, which shows enhanced expression.…”

Section: Discussionmentioning

confidence: 95%

Temporal profiling of the transcriptional basis for the development of corticosteroid-induced insulin resistance in rat muscle

Almon

DuBois²,

Jin³

et al. 2005

Journal of Endocrinology

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Elevated systemic levels of glucocorticoids are causally related to peripheral insulin resistance. The pharmacological use of synthetic glucocorticoids (corticosteroids) often results in insulin resistance/type II diabetes. Skeletal muscle is responsible for close to 80% of the insulininduced systemic disposal of glucose and is a major target for glucocorticoid-induced insulin resistance. We used Affymetrix gene chips to profile the dynamic changes in mRNA expression in rat skeletal muscle in response to a single bolus dose of the synthetic glucocorticoid methylprednisolone. Temporal expression profiles (analyzed on individual chips) were obtained from tissues of 48 drugtreated animals encompassing 16 time points over 72 h following drug administration along with four vehicletreated controls. Data mining identified 653 regulated probe sets out of 8799 present on the chip. Of these 653 probe sets we identified 29, which represented 22 gene transcripts, that were associated with the development of insulin resistance. These 29 probe sets were regulated in three fundamental temporal patterns. 16 probe sets coding for 12 different genes had a profile of enhanced expression. 10 probe sets coding for eight different genes showed decreased expression and three probe sets coding for two genes showed biphasic temporal signatures. These transcripts were grouped into four general functional categories: signal transduction, transcription regulation, carbohydrate/fat metabolism, and regulation of blood flow to the muscle. The results demonstrate the polygenic nature of transcriptional changes associated with insulin resistance that can provide a temporal scaffolding for translational and post-translational data as they become available.

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Section: Discussionmentioning

confidence: 95%

Temporal profiling of the transcriptional basis for the development of corticosteroid-induced insulin resistance in rat muscle

Almon

DuBois²,

Jin³

et al. 2005

Journal of Endocrinology

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“…The β-subunit shows 3-oxoacyl-CoA thiolase activity, and the α-subunit provides enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activity. Concerning dietary regulation of this gene, Oshida et al [38] reported that HADHB activity in muscle was higher in rats fed a high sucrose diet. According to them, the high sucrose group exhibited a metabolic pattern in muscle that favoured carbohydrate over fat oxidation.…”

Section: Discussionmentioning

confidence: 99%

Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis

et al. 2010

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BackgroundThe expression of some genes controlling energy homeostasis could be regulated by epigenetic mechanisms that may play a role in body weight regulation. Thus, it is known that various nutritional factors affect DNA methylation. In order to assess whether the macronutrient composition of the diet could be related to the epigenetic regulation of gene expression and with obesity development, we investigated the effects on methylation and expression patterns of two pair-fed isocaloric diets in rats: control (rich in starch) and HFS (rich in fat and sucrose).ResultsThe pair-fed HFS diet induced higher weight gain and adiposity as compared to the controls as well as liver triglyceride accumulation and oxidative stress. Feeding the HFS diet impaired glucose tolerance and serum triglycerides and cholesterol. Liver glucokinase expression, a key glycolytic gene, remained unaltered, as well as the mRNA values of fatty acid synthase and NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6 (NDUFB6) in liver and visceral adipocytes, which regulate lipogenesis and mitochondrial oxidative metabolism, respectively. Liver expression of hydroxyacyl-coenzyme A dehydrogenase (HADHB), a key gene of β-oxidation pathway, was higher in the HFS-fed animals. However, the methylation status of CpG islands in HADHB and glucokinase genes remained unchanged after feeding the HFS diet.ConclusionsThese results confirm that the distribution and type of macronutrients (starch vs. sucrose, and percent of fat) influence obesity onset and the associated metabolic complications. HFS diets produce obesity independently of total energy intake, although apparently no epigenetic (DNA methylation) changes accompanied the modifications observed in gene expression.

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“…Treatment with the insulin sensitizer troglitazone produces a rise in the metabolic clearance rate of glucose during a euglycemic hyperinsulinemic clamp in obese rats and Zucker diabetic rats. 4,5) Troglitazone increases the muscle glycogen content after the clamp, compared with untreated obese rats. Troglitazone may improve insulin sensitivity associated with improvements in intracellular substrate use and energy stores.…”

Section: Discussionmentioning

confidence: 99%

“…These compounds increase peripheral glucose uptake while decreasing gluconeogenesis of the liver in a wide variety of experimental models of type 2 diabetes and in humans. 4,5) The compounds improve glucose tolerance and insulin sensitivity in both diabetic and glucose-intolerant patients. However, troglitazone, one of this class compounds, can cause liver damage and multiple progenies in humans, and is no longer prescribed.…”

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confidence: 99%

A Steroidal Glycoside fromPolygonatum odoratum(Mill.) Druce. Improves Insulin Resistance but does not Alter Insulin Secretion in 90% Pancreatectomized Rats

Choi

Park

2002

Bioscience, Biotechnology, and Biochemistry

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The aim of this study was to investigate the effects of three steroidal glycosides (SG-100, SG-280, and SG-460) obtained from Polygonatum odoratum (Mill.) Druce. on insulin secretion, insulin action, and relative glucose uptake in various tissues of 90% pancreatectomized male Sprague-Dawley rats. One of the compounds (30 mg/kg body weight daily) with a 40%-fat diet was orally administered to a group of such rats for 13 weeks. On the day after a hyperglycemic clamp, euglycemic hyperinsulinemic clamp with 1 microCi of [1-(14)C]2-deoxyglucose per 100 g body weight was used. Serum glucose levels were lowest in the rats receiving SG-100. Insulin secretion from pancreatic beta-cells did not change with SG administration. Whole-body glucose disposal rates increased with SG-100 administration by 39%. SG-100 increased the glycogen contents and glycogen synthase activity in the soleus muscle of pancreatectomized rats. Uptake of [1-(14)C]2-deoxyglucose into the soleus muscle was higher in such rats receiving SG-100 than in rats receiving other compounds. In conclusion, SG-100 has an antihyperglycemic effect by promoting peripheral insulin sensitivity without changing insulin secretion.

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Troglitazone Improves Insulin-Stimulated Glucose Utilization Associated with an Increased Muscle Glycogen Content in Obese Zucker Rats.

Cited by 15 publications

References 37 publications

Temporal profiling of the transcriptional basis for the development of corticosteroid-induced insulin resistance in rat muscle

Temporal profiling of the transcriptional basis for the development of corticosteroid-induced insulin resistance in rat muscle

Obesity induced by a pair-fed high fat sucrose diet: methylation and expression pattern of genes related to energy homeostasis

A Steroidal Glycoside fromPolygonatum odoratum(Mill.) Druce. Improves Insulin Resistance but does not Alter Insulin Secretion in 90% Pancreatectomized Rats

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