Trophinin-associated protein expression correlates with shorter survival of patients with glioma: a study based on multiple data fusion analysis

Sun, Yong; Liu, Zhen Dong; Liu, Run Ze; Lian, Xiao Yu; Cheng, Xing Bo; Jia, Yu Long; Liu, Bin Feng; Gao, Yanzheng; Wang, Xinjun

doi:10.1007/s11033-022-07622-8

Cited by 1 publication

(1 citation statement)

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“…In addition, a report applying multiple data fusion analyses demonstrated elevated tastin amounts are correlated with shorter survival in glioma cases. 27 Moreover, tastin expression was suggested to independently predict prognosis in lung adenocarcinoma cases in a study by Chen and colleagues. 28 As shown above, tastin levels were elevated in tumor samples, especially NSCLC, and increased tastin expression was associated with poorer patient prognosis.…”

Section: Discussionmentioning

confidence: 99%

Tastin promotes non-small-cell lung cancer progression through the ErbB4, PI3K/AKT, and ERK1/2 pathways

Yue

Chen

Dai

et al. 2023

Exp Biol Med (Maywood)

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Tastin might be involved in tumorigenesis, but its role in non-small-cell lung cancer (NSCLC) has not been adequately explored. This work aimed to examine tastin’s role in NSCLC and to explore the underlying mechanism. The Gene Expression Omnibus (GEO), Gene Expression Database of Normal and Tumor tissues (GENT), and Cancer Genome Atlas (TCGA) databases were used. Four GEO datasets (GSE81089, GSE40419, GSE74706, and GSE19188) containing gene expression data for NSCLC and normal tissue samples were analyzed for tastin mRNA expression. Tastin expression levels in different tissues were compared using the GENT website. TCGA biolinks were used to download gene expression quantification ( n = 594) and overall survival data ( n = 535). In total, 30 lung adenocarcinoma and 25 lung squamous cell carcinoma cases were enrolled. In addition, four-week-old male BALB/c nude mice ( n = 9/group) were used to establish xenograft mouse models. Furthermore, cultured HEK293T, A549, and NCI-H226 cells assessed. Immunoblot, hematoxylin and eosin (H&E) staining, immunohistochemistry, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), fluorescence microscopy, flow cytometry, lentiviral transduction, and MTT, colony formation, wound healing, and Transwell assays were carried out. Tastin expression levels were markedly increased in NSCLC tumor tissue specimens and correlated with a poorer prognosis. Silencing of tastin inhibited the proliferative and migratory abilities of NSCLC cells. Bioinformatic analysis suggested that tastin interacts with ErbB4. The PI3K/AKT and ERK1/2 downstream pathways were suppressed in tastin-deficient cells. In conclusion, tastin might be involved in NSCLC growth and invasion and is a potential therapeutic target in NSCLC.

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Section: Discussionmentioning

confidence: 99%