Trophoblast giant cells express NF-?B2 during early mouse development

Muggia, Anna; Teesalu, Tambet; Neri, Antonino; Blasi, Francesco; Talarico, Daniela

doi:10.1002/(sici)1520-6408(1999)25:1<23::aid-dvg3>3.0.co;2-k

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…Binding sites for the transcription factors PLAG1, RELA and RREB1 were enriched for genes overexpressed in ICM while binding sites for nine transcription factors (EGR1, GABPA, KLF4, MYF, SP1, MZF1, NHLH1, PAX5 and ZFX) were significantly enriched for TE. RELA is a subunit for NFκB, which in turn has been implicated in differentiation of trophoblast lineages from embryonic stem cells [65] and in function of trophoblast giant cells [66]. Several of the transcription factors associated with genes upregulated in TE are involved in hematopoiesis, including EGR1 [67], GABPA [68], MZF1 [69], and ZFX [70].…”

Section: Discussionmentioning

confidence: 99%

Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

et al. 2012

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BackgroundThe first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system.ResultsA total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.ConclusionAnalysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.

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Section: Discussionmentioning

confidence: 99%

Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

et al. 2012

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“…BCL-3 is implicated in the remodelling of the uterus for blastocyst implantation through negatively regulating TNF-α transcription, which is necessary for the dynamic regulation of NF-κB activity in the uterus to maintain a favourable environment of cytokines for pregnancy preparation [ 31 ]. BCL-3 is expressed in 7–10-day mouse embryo implantation sites, and detected over decidua at 7–8 days post coitum, but has weak labelling at 10 days post coitum [ 37 ]. In this study, early pregnancy induced upregulation of BCL-3 at DP16, but downregulation at DP25 in the maternal liver.…”

Section: Discussionmentioning

confidence: 99%

Expression of IkappaB Family in the Ovine Liver during Early Pregnancy

Cai

Ren

Cao

et al. 2023

Animals

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During normal pregnancy, there is a dynamic regulation of the maternal immune system, including the liver, to accommodate the presence of the allogeneic foetus in the uterus. However, it was unclear that the expression of the IkappaB (IκB) family was regulated in the ovine maternal liver during early pregnancy. In this study, sheep livers were collected at day 16 of the oestrous cycle (NP16), and days 13, 16 and 25 of gestation (DP13, DP16 and DP25), and RT-qPCR, Western blot and immunohistochemistry analysis were used to analyse the expression of the IκB family, including B cell leukemia-3 (BCL-3), IκBα, IκBβ, IκBε, IKKγ, IκBNS and IκBζ. The results revealed that expression of BCL-3, IκBβ, IκBε and IKKγ peaked at DP16, and the expression of IκBα was increased during early pregnancy. In addition, the expression of IκBζ peaked at DP13 and DP16, and IκBNS peaked at DP13. IκBβ and IKKγ proteins were located in the endothelial cells of the proper hepatic arteries and portal veins, and hepatocytes. In conclusion, early pregnancy changed the expression of the IκB family, suggesting that the modulation of the IκB family may be related to the regulation of maternal hepatic functions, which may be favourable for pregnancy establishment in sheep.

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“…It is possible that ROS regulate the activity of transcriptions factors, such as nuclear factor‐kappaB (NF‐κB), which is sensitive to redox changes (Gloire & Piette, ). NF‐κB was found to be expressed in the trophoblast during mouse blastocyst implantation (Muggia, Teesalu, Neri, Blasi, & Talarico, ). The promoter regions of PLAU and MMP‐9 contain a binding element to NF‐κB (Gutsche et al, ; Tobar et al, ; Tronov, Konstantinov, Petrakou, Tsilimigaki, & Piperakis, ; Uemura et al, ), which could explain the increase in MMP‐9 and PLAU levels in mouse conceptuses.…”

Section: Discussionmentioning

confidence: 99%

Hyperglycemia‐induced mouse trophoblast spreading is mediated by reactive oxygen species

Sánchez-Santos

Martínez-Hernández

Contreras-Ramos³

et al. 2018

Molecular Reproduction Devel

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During embryo implantation, the outer layer of the blastocyst interacts with the endometrium giving rise to the development of the trophoblast cell lineage. The cells in this lineage participate in the penetration of endometrium due to their motility and invasive properties. The mechanisms that regulate the differentiation and invasive ability of these cells are essential for the establishment and maintenance of an efficient exchange between maternal and fetal tissues during pregnancy. In this context, hyperglycemia can induce oxidative stress causing alterations in the placenta. This study evaluated the role of reactive oxygen species (ROS) in the actions of high glucose concentration (HG) on trophoblast spreading and the expression of extracellular proteases in cultured mouse conceptuses. Blastocysts from gestational day 4 (GD4) were cultured until GD7 in HAM-F10 medium and further treated for 48 hr with HG (25 mM glucose) from GD7 to GD9. This treatment induced larger trophoblast outgrowths and increased ROS concentration, which was associated with increased expression levels of urokinase-type plasminogen activator (PLAU), plasminogen activator inhibitor 1 (PAI-1), and matrix metalloproteinase 9 (MMP-9). These effects were prevented by treatment with the non-specific antioxidant N-acetylcysteine (NAC) or apocynin, an inhibitor of NADPH oxidase. Our data suggest that the HG-induced trophoblast spreading and the expression of PLAU, PAI-1, and MMP-9 were mediated by the production of ROS via NADPH oxidase activity. Our results shed light on placental alterations in gestational diabetes mellitus.

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Trophoblast giant cells express NF-?B2 during early mouse development

Cited by 9 publications

References 44 publications

Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

Expression of IkappaB Family in the Ovine Liver during Early Pregnancy

Hyperglycemia‐induced mouse trophoblast spreading is mediated by reactive oxygen species

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