In the study of animal behavior, the prevention of sickness, and the gait planning of legged robots, pose estimation, and gait parameter extraction of quadrupeds are of tremendous importance. However, there are several varieties of quadrupeds, and distinct species frequently have radically diverse body types, limb configurations, and gaits. Currently, it is challenging to forecast animal pose estimation with any degree of accuracy. This research developed a quadruped animal pose estimation and gait parameter extraction method to address this problem. A computational framework including three components of target screening, animal pose estimation model, and animal gaits parameter extraction, which can totally and efficiently solve the problem of quadruped animal pose estimation and gait parameter extraction, makes up its core. On the basis of the HRNet network, an improved quadruped animal keypoint extraction network, RFB-HRNet, was proposed to enhance the extraction effect of quadruped pose estimation. The basic concept was to use a DyConv (dynamic convolution) module and an RFB (receptive field block) module to propose a special receptive field module DyC-RFB to optimize the feature extraction capability of the HRNet network at stage 1 and to enhance the feature extraction capability of the entire network model. The public dataset AP10K was then used to validate the model’s performance, and it was discovered that the proposed method was superior to alternative methods. Second, a two-stage cascade network was created by adding an object detection network to the front end of the pose estimation network to filter the animal object in input images, which enhanced the pose estimation effect of small targets and multitargets. The acquired keypoints data of animals were then utilized to extract the gait parameters of the experimental objects. Experiment findings showed that the gait parameter extraction model proposed in this research could effectively extract the gait frequency, gait sequence, gait duty cycle, and gait trajectory parameters of quadruped animals, and obtain real-time and accurate gait trajectory.