Trout P450IA1: cDNA and Deduced Protein Sequence, Expression in Liver, and Evolutionary Significance

Heilmann, Larry J.; Sheen, Yi‐Shuan; Bigelow, Sanford W.; Nebert, Daniel W.

doi:10.1089/dna.1.1988.7.379

Cited by 210 publications

(64 citation statements)

References 25 publications

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“…as listed in Genebank. This sequence differs by 3 nucleotides from the corresponding segment of trout P4501A1 (Heilmann et al 1988). The probe was labelled with ($'P)-ATP by the method of Maxam & Gilbert (1980).…”

Section: Methodsmentioning

confidence: 99%

Hepatic microsomal mono-oxygenase activity and P450IA mRNA in North Sea dab Limanda limanda from contaminated sites

Kw¹,

Addison²

1992

Mar. Ecol. Prog. Ser.

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Mature pre-spawning female dab Limanda limanda were sampled during the Bremerhaven Workshop from stations in the southern North Sea along a transect heading approxin~ately NW from the Elbe estuary (the 'German Bight Transect') and around an abandoned drilling s~t e .Hepatic rnicrosomal mono-oxygenase activity as shown by ethoxyresorufin 0-deethylase (EROD) and cyanoethoxycoumarin 0-deethylase (CN-ECOD) declined with increasing distance offshore, but rose slightly a t the outermost station on the Dogger Bank. Microsomal P4501A1 mRNA, detected with a new synthetic oligonucleotide probe, followed the same trend. Activities of EROD and CN-ECOD and concentrations of P4501A1 mRNA were well correlated with the major chlorobiphenyl residues measured in dab and with hydrocarbon concentrations in sedlments at these stations. Dab sampled from the drilling site had hepatic EROD and CN-ECOD activ~ty and P4501A1 mRNA contents similar to those in fish from the offshore German Bight stations.

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Section: Methodsmentioning

confidence: 99%

Hepatic microsomal mono-oxygenase activity and P450IA mRNA in North Sea dab Limanda limanda from contaminated sites

Kw¹,

Addison²

1992

Mar. Ecol. Prog. Ser.

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“…Lesions (9,124), and (e) many liver and skin lesions seen in epizootics are similar to those classically described in rodent carcinogenesis. In addition, the resistance of related species in similar habitats to the development of neoplasms indicates differing species susceptibilities (33,134 (84,212), scup (Stenotomus chrysops) (104,105), cod (Gadus morhua) (65), English sole (158), and other species (185). The major Phase II routes of metabolism for electrophilic and nucleophilic compounds in fishes are glutathione and glucuronic acid conjugation, respectively (64).…”

Section: Epizootic Liver Neoplasiamentioning

confidence: 99%

Review Article: Experimental Chemical Carcinogenesis in Fish

Bunton

1996

Toxicol Pathol

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Dr. Bunton's primary research interest is on mechanisms of toxicity and carcinogenesis in fish related to environmental contamination and on the development of alternative models.Experimental carcinogenesis using fish species as alternative models is a dynamic field of research. The 1940's expansion of synthetic chemical producing industries coincided with a number of pollution-associated fish neoplasia epizootics, with polycyclic aromatic hydrocarbons as significant components of contaminated sediment in several cases. Epizootics of primarily liver and skin neoplasia in benthic species near coastal urban or industrial areas indicated the sensitivity of fish species to known mammalian carcinogens. Stressing a mechanistic approach, investigators have used data compiled from epizootics as the backbone of current research efforts to define carcinogenesis in fish species. With liver as the focus, patterns of neoplastic development similar to those seen in rodent bioassays have been induced in various fish species by genotoxic carcinogens. Similarities between fish and rodent models include chemical and species-specific responses to exposure and the development of predictable preneoplastic and neoplastic lesions. The expression of molecular molecules related to carcinogenesis is currently under investigation, which includes alterations in certain proteins, enzyme activity, and oncogene/tumor suppressor gene function. The potential for the application of research findings to both human and environmental health issues makes fish species attractive and valuable alternative models in carcinogenesis and toxicity research.Keywords. Medaka; methylazoxymethanol (MAM); molecular markers; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG); Neoplasia; polycyclic aromatic hydrocarbons (PAH); rainbow trout INTRODUCTION Armed with data from fish neoplasia epizootics related to environmental synthetic chemical contamination, research has moved from the morphologic assessment of neoplasms to the identification of molecular mechanisms of neoplastic development and progression. The value of these studies can be argued along 2 lines that are often, but should not be, separated. One is for application to human health issues for which there is a chronic demand, and the other is the inherent value of monitoring the health of the environment, which includes all of the creatures therein. Unfortunately, it is the latter argument that most often has to be vigorously defended, although minimal reflection will reveal that we, too, are members of this population.The present review discusses experimental fish carcinogenesis by first presenting data from neoplasia epizootics that helped to form the framework of our current 604 research efforts. The effects of known direct and indirect carcinogens on various fish species from laboratory studies are then described to illustrate species similarities and differences in tissue response. The latter portion of the paper concentrates on chemical hepatocarcinogenesis and reviews the histologic and molecular marke...

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“…CYP1A nucleotide sequences have been determined in rainbow trout (Berndtson and Chen, 1994;Heilmann et al, 1988), plaice (Leaver et al, 1993), Atlantic tomcod (Roy et al, 1995), toadfish and scup (Morrison et al, 1995), killifish (Morrison et al, 1998), red sea bream (Mizukami et al, 1994), sea bass (Stien et al, 1998) and, more recently, Atlantic salmon (Rees et al, 2003). Current evidence shows that several CYP1A isoforms exist in fish (Teramitsu et al, 2000) and they are distributed in tissues such as the liver, gut, kidney, gill and heart (Goksøyr and Husoy, 1998;Sarasquete and Segner, 2000;Stegeman and Hahn, 1994).…”

mentioning

confidence: 99%

β-naphthoflavone induction of CYP1A in brain of juvenile lake trout(Salvelinus namaycush Walbaum)

Chung-Davidson

Rees

et al. 2004

Journal of Experimental Biology

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Many environmental pollutants induce expression of the cytochrome P450 (CYP) 1A subfamily of genes. We integrated cellular and molecular biological techniques to examine the effects of β-naphthoflavone (BNF) exposure in lake trout brain CYP1A distribution and dynamics. Over a 32-day time-course, real time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) results showed that CYP1A mRNA induction in response to BNF exposure occurred rapidly and continued to rise in the BNF-treated lake trout after 4·h, with a peak at or after 2·days. Messenger RNA levels fell after 4·days, and this trend continued after 16·days of exposure. In situ hybridization indicated that CYP1A mRNA was universally elevated in the brain of BNF-exposed fish and was mainly expressed in the endothelia and occasionally in the glial cells. CYP1A immunoreactivity was induced in the olfactory bulb and valvula cerebelli of BNF-treated fish. Other brain areas showed constitutive CYP1A immunoreactivity in both control and BNF-treated fish. Some BNF-treated fish contained multifocal hemorrhages in the brain tissue, and these fish had overall depressed CYP1A immunoreactivity in the brain. The relationship between transcriptional and translational effects of BNF exposure in the brain of juvenile lake trout is discussed.

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Trout P450IA1: cDNA and Deduced Protein Sequence, Expression in Liver, and Evolutionary Significance

Cited by 210 publications

References 25 publications

Hepatic microsomal mono-oxygenase activity and P450IA mRNA in North Sea dab Limanda limanda from contaminated sites

Hepatic microsomal mono-oxygenase activity and P450IA mRNA in North Sea dab Limanda limanda from contaminated sites

Review Article: Experimental Chemical Carcinogenesis in Fish

β-naphthoflavone induction of CYP1A in brain of juvenile lake trout(Salvelinus namaycush Walbaum)

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