TRPM2-AS promotes the malignancy of osteosarcoma cells by targeting miR-15b-5p/PPM1D axis

Cai, Yingchun; Yang, Yudan; Zhang, Xudong; Ma, Qingqing; Li, Mengyi

doi:10.1080/15384101.2022.2033414

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…[30] Similarly, another study demonstrated that TRPM2-AS promotes malignant tumors in OS cells by targeting the miR-15b-5p/PPM1D axis. [31] The above studies are consistent with our findings: MCOLN2 is highly expressed in colorectal cancer and OS, and the higher the MCOLN2, the worse the prognosis.…”

Section: Discussionsupporting

confidence: 92%

Role of PAX6, TRPA1, BCL11B, MCOLN2, CUX1, EMX1 in colorectal cancer and osteosarcoma

Zhang,

Gao,

Hou

et al. 2024

Medicine

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Colorectal cancer is a cancer that arises from the abnormal growth of cells in the colon or rectum. Osteosarcoma (OS) is a common primary bone tumor with high degree of malignancy. The configuration files for colorectal cancer dataset GSE142279 and OS datasets GSE197158 and GSE206448 were downloaded from Gene Expression Omnibus database using the platforms GPL20795, GPL20301, and GPL24676. Differentially expressed genes (DEGs) were screened and weighted gene co-expression network analysis (WGCNA) was performed. Construction and analysis of protein-protein interactions (PPI) network. Functional enrichment analysis, gene set enrichment analysis (GSEA) were performed. A heat map of gene expression was drawn. The Comparative Toxicogenomics Database (CTD) was used to find the diseases most associated with the core genes. TargetScan was used to screen miRNAs regulating DEGs. According to the Gene Ontology (GO) analysis, DEGs are mainly enriched in acetylcholine binding receptor activity involved in Wnt signaling pathway, cell polarity pathway, PI3K-Akt signaling pathway, receptor regulator activity, cytokine-cytokine receptor interaction, transcriptional misregulation in cancer, and inflammation-mediated regulation of tryptophan transport. In the Metascape enrichment analysis, GO enrichment items related to the regulation of Wnt signaling pathway, regulation of muscle system process, and regulation of actin filament-based movement. Eight core genes (CUX1, NES, BCL11B, PAX6, EMX1, MCOLN2, TRPA1, TRPC4) were identified. CTD showed that 4 genes (CUX1, EMX1, TRPA1, BCL11B) were associated with colorectal neoplasms, colorectal tumors, colonic diseases, multiple myeloma, OS, and inflammation. PAX6, TRPA1, BCL11B, MCOLN2, CUX1, and EMX1 are highly expressed in colorectal cancer and OS, and the higher the expression level, the worse the prognosis.

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Section: Discussionsupporting

confidence: 92%

Role of PAX6, TRPA1, BCL11B, MCOLN2, CUX1, EMX1 in colorectal cancer and osteosarcoma

Zhang,

Gao,

Hou

et al. 2024

Medicine

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“…In our data, the expression of miR-15b-5p was significantly lower in patients with HSCC than that in the normal population. Previous studies have indicated that the lncRNA TRPM2-AS/miR-15b-5p/PPM1D axis can promote malignant tumors in osteosarcoma cells 45 or Linc00662 can act as a sponge for miR-15b-5p , facilitating the progression of osteoarthritis 46 . Additionally, lncRNA-MEG8 accelerates non-small cell lung cancer progression by regulating the miR-15b-5p /PSAT1 axis 47 .…”

Section: Discussionmentioning

confidence: 99%

Linc00662 sponges miR-15b-5p to promote hypopharyngeal squamous cell carcinoma progression by facilitating cancer stem cell-like phenotypes

Zhang,

2024

J. Cancer

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Background: Long non-coding RNAs (lncRNAs) are associated with multiple head and neck tumors and play important roles in cancer. This study explored the molecular mechanism of Linc00662 in hypopharyngeal squamous cell carcinoma (HSCC). Methods: Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect gene expression in HSCC tissues. The viability and proliferation of tumor cells were measured using CCK-8 assays. HSCC cell apoptosis was measured using flow cytometry and western blotting. Cell stemness was examined using the sphere formation assay. A xenograft tumor model was established to investigate the role of Linc00662 in vivo. Results:The expression level of Linc00662 in HSCC tissues was significantly higher than that in adjacent normal tissues. The expression of Linc00662 had no significant relationship with the tumor stage. Patients with high Linc00662 expression were found to have shorter overall survival than those with low Linc00662 expression. Linc00662 over-expression promoted cell viability and inhibited apoptosis. Using online databases and a dual luciferase reporter, miR-15b-5p was confirmed as a potential downstream sponge of Linc00662. Moreover, Linc00662 was negatively associated with miR-15b-5p in HSCC cells. Depletion of miR-15b-5p can reverse the function of Linc00662 in vivo and in vitro. Furthermore, Linc00662 promotes tumor growth, which was abolished by miR-15b-5p mimics. Importantly, the stemness of cancer stem cells was mediated by the Linc00662/miR-15b-5p axis. Conclusion: Patients with HSCC with high Linc00662 showed poor prognosis and high Linc00662 induced stemness of tumor cells by targeting miR-15b-5p. Linc00662 may serve as a novel diagnostic and target marker for head and neck squamous cell carcinoma.

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“…The expression and function of miR-15b-5p can be extensive depending on hormone status in PCa. Notably, recent studies have shown that several noncoding RNAs adsorb miR-15b-5p, thereby reducing its expression in cells; TTN-AS1 in ovarian cancer and TRPM2-AS in osteosarcoma [24][25][26]. Recent study showed that the expression of AFAP1-AS1 was elevated in CRPC cell lines compared with hormone-sensitive LNCaP cells.…”

Section: Discussionmentioning

confidence: 99%

“…We next questioned the possible mechanisms for downregulation of miR-15b-5p in castrated condition. According to the previous studies, we selected three types of lncRNAs (TTN-AS1, TRPM2-AS and AFAP-AS1) that have the potential to adsorb miR-15b-5p and suppress its function [24][25][26]. Among these three lncRNAs, AFAP1-AS1 was significantly upregulated in CRPC cells compared to LNCaP (Fig.…”

Section: Mir-15b-5p Directly Bound To Afap1-as1 In Pc3 Cellsmentioning

confidence: 99%

MiR‐15b‐5p inhibits castration‐resistant growth of prostate cancer cells by targeting the muscarinic cholinergic receptor CHRM3

et al. 2023

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Cholinergic receptor muscarinic 3 (CHRM3)‐mediated focal adhesion kinase/YES‐associated protein (YAP) signalling is essential for the growth of castration‐resistant prostate cancer (CRPC) cells. Here, we evaluated the molecular mechanisms through which CHRM3 overexpression facilitates castration‐resistant growth. Small RNA sequencing combined with in silico analyses revealed that CHRM3 was a putative target of miR‐15b‐5p. Notably, androgen deprivation suppressed miR‐15b‐5p expression and increased CHRM3 expression. Moreover, miR‐15b‐5p bound directly to CHRM3 and inhibited YAP activation induced by CHRM3 stimulation. Furthermore, miR‐15b‐5p abolished the growth of CRPC cells induced by CHRM3 stimulation. We conclude that the miR‐15b‐5p/CHRM3/YAP signalling axis promotes the castration‐resistant growth of prostate cancer.

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TRPM2-AS promotes the malignancy of osteosarcoma cells by targeting miR-15b-5p/PPM1D axis

Cited by 8 publications

References 42 publications

Role of PAX6, TRPA1, BCL11B, MCOLN2, CUX1, EMX1 in colorectal cancer and osteosarcoma

Role of PAX6, TRPA1, BCL11B, MCOLN2, CUX1, EMX1 in colorectal cancer and osteosarcoma

Linc00662 sponges miR-15b-5p to promote hypopharyngeal squamous cell carcinoma progression by facilitating cancer stem cell-like phenotypes

MiR‐15b‐5p inhibits castration‐resistant growth of prostate cancer cells by targeting the muscarinic cholinergic receptor CHRM3

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