TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium–calcium exchangers in rat odontoblasts

Tsumura, Maki; Ubaidus, Sobhan; Muramatsu, Takashi; Sato, Masaki; Ichikawa, Hideki; Sahara, Yoshinori; Tazaki, Masakazu; Shibukawa, Yoshiyuki

doi:10.1016/j.ceca.2012.05.002

Cited by 76 publications

(88 citation statements)

References 49 publications

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“…Therefore, dentinal pain with a short latency (within 1 sec) following various stimuli, including thermal, chemical, osmotic, and mechanical, on the dentin surface results from dentinal fluid movement [6], [29], [31]. This suggests that mechanosensors such as TRPV1, TRPV2, and TRPV4 [11], [13] as well as TRPA1 channels in odontoblasts detecting movement of dentinal fluid may be necessary for dentinal sensation [6], [29], [31].…”

Section: Discussionmentioning

confidence: 99%

“…The primary cultured odontoblasts in the dental pulp slice were used for [Ca 2+ ] i measurement within 24 hr after isolation. These cells were positive for the odontoblast markers dentin matrix protein-1, dentin sialoprotein, and nestin [13] (not shown) and were thus odontoblasts.…”

Section: Methodsmentioning

confidence: 98%

“…Dental pulp slice preparations were obtained from newborn Wistar rats (3- to 10-day-old) using a previously described method [13]. Briefly, under pentobarbital sodium anesthesia (25 mg/kg), the mandible was dissected.…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies demonstrated that acutely isolated rat and mouse odontoblasts, cultured human dental pulp cells, and cultured mouse odontoblast lineage cells express TRP vanilloid (TRPV) channels. These TRPV channels respond to pH-related, thermal, or hypotonic (membrane stretching) stimulation; extracellular stimulants; or endogenous agonists [5], [9]–[13]. Thus, the expression and the thermal, pharmacological, and mechanical sensitivities of TRPV channel subfamilies in odontoblasts have been well documented.…”

Section: Introductionmentioning

confidence: 99%

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Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

et al. 2013

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Odontoblasts produce dentin during development, throughout life, and in response to pathological conditions by sensing stimulation of exposed dentin. The functional properties and localization patterns of transient receptor potential (TRP) melastatin subfamily member 8 (TRPM8) and ankyrin subfamily member 1 (TRPA1) channels in odontoblasts remain to be clarified. We investigated the localization and the pharmacological, biophysical, and mechano-sensitive properties of TRPM8 and TRPA1 channels in rat odontoblasts. Menthol and icilin increased the intracellular free Ca2+ concentration ([Ca2+]i). Icilin-, WS3-, or WS12-induced [Ca2+]i increases were inhibited by capsazepine or 5-benzyloxytriptamine. The increase in [Ca2+]i elicited by allyl isothiocyanate (AITC) was inhibited by HC030031. WS12 and AITC exerted a desensitizing effect on [Ca2+]i increase. Low-temperature stimuli elicited [Ca2+]i increases that are sensitive to both 5-benzyloxytriptamine and HC030031. Hypotonic stimulation-induced membrane stretch increased [Ca2+]i; HC030031 but not 5-benzyloxytriptamine inhibited the effect. The results suggest that TRPM8 channels in rat odontoblasts play a role in detecting low-temperature stimulation of the dentin surface and that TRPA1 channels are involved in sensing membrane stretching and low-temperature stimulation. The results also indicate that odontoblasts act as mechanical and thermal receptor cells, detecting the stimulation of exposed dentin to drive multiple cellular functions, such as sensory transduction.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

et al. 2013

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“…Anandamide acts primarily on cannabinoid receptor type 1 (CB 1 )[1], cannabinoid receptor type 2 (CB 2 )[2], and ionotropic receptors, including the transient receptor potential (TRP) vanilloid type 1 (TRPV1)[345], TRP ankyrin type 1 (TRPA1) and TRP melastatin type 8 (TRPM8) channels[6]. Anandamide can also directly modulate various other ion channels[578910]. …”

Section: Introductionmentioning

confidence: 99%

Regulatory effects of anandamide on intracellular Ca ²⁺ concentration increase in trigeminal ganglion neurons

et al. 2014

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Activation of cannabinoid receptor type 1 on presynaptic neurons is postulated to suppress neurotransmission by decreasing Ca2+ influx through high voltage-gated Ca2+ channels. However, recent studies suggest that cannabinoids which activate cannabinoid receptor type 1 can increase neurotransmitter release by enhancing Ca2+ influx in vitro. The aim of the present study was to investigate the modulation of intracellular Ca2+ concentration by the cannabinoid receptor type 1 agonist anandamide, and its underlying mechanisms. Using whole cell voltage-clamp and calcium imaging in cultured trigeminal ganglion neurons, we found that anandamide directly caused Ca2+ influx in a dose-dependent manner, which then triggered an increase of intracellular Ca2+ concentration. The cyclic adenosine and guanosine monophosphate-dependent protein kinase systems, but not the protein kinase C system, were involved in the increased intracellular Ca2+ concentration by anandamide. This result showed that anandamide increased intracellular Ca2+ concentration and inhibited high voltage-gated Ca2+ channels through different signal transduction pathways.

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Sperm Release From the Oviductal Epithelium Depends on Ca²⁺Influx Upon Activation of CB1 and TRPV1 by Anandamide

et al. 2015

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The oviduct acts as a functional sperm reservoir in many mammalian species. Both binding and release of spermatozoa from the oviductal epithelium are mainly modulated by sperm capacitation. Several molecules from oviductal fluid are involved in the regulation of sperm function. Anandamide is a lipid mediator involved in reproductive physiology. Previously, we demonstrated that anandamide, through activation of the cannabinoid receptor type 1 (CB1), promotes sperm release from bovine oviductal epithelial cells, and through CB1 and the transient receptor potential vanilloid 1 (TRPV1), induces sperm capacitation. Herein we investigate co-activation between CB1 and TRPV1, and Ca(2+) influx as part of the mechanism of action of anandamide during sperm release from oviductal cells. Our results indicate that in the absence of Ca(2+) anandamide failed to release spermatozoa from oviductal epithelial cells. Additionally, sperm release promoted by cannabinoid and vanilloid agonists was abolished when the spermatozoa were preloaded with BAPTA-AM, a Ca(2+) chelator. We also determined Ca(2+) levels in spermatozoa preloaded with FURA2-AM co-cultured with oviductal cells and incubated with different cannabinoid and vanilloid agonists. The incubation with different agonists induced Ca(2+) influx, which was abolished by CB1 or TRPV1 antagonists. Our results also suggest that a phospholypase C (PLC) might mediate the activation of CB1 and TRPV1 in sperm release from the bovine oviduct. Therefore, our findings indicate that anandamide, through CB1 and TRPV1 activation, is involved in sperm release from the oviductal reservoir. An increase of sperm Ca(2+) levels and the PLC activation might be involved in anandamide signaling pathway.

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TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium–calcium exchangers in rat odontoblasts

Cited by 76 publications

References 49 publications

Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

Regulatory effects of anandamide on intracellular Ca ²⁺ concentration increase in trigeminal ganglion neurons

Sperm Release From the Oviductal Epithelium Depends on Ca²⁺Influx Upon Activation of CB1 and TRPV1 by Anandamide

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TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium–calcium exchangers in rat odontoblasts

Cited by 76 publications

References 49 publications

Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

Regulatory effects of anandamide on intracellular Ca 2+ concentration increase in trigeminal ganglion neurons

Sperm Release From the Oviductal Epithelium Depends on Ca2+Influx Upon Activation of CB1 and TRPV1 by Anandamide

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Regulatory effects of anandamide on intracellular Ca ²⁺ concentration increase in trigeminal ganglion neurons

Sperm Release From the Oviductal Epithelium Depends on Ca²⁺Influx Upon Activation of CB1 and TRPV1 by Anandamide