TRPV1, Targeted by miR-338-3p, Induces Neuropathic Pain by Interacting with NECAB2

Ma, Yuetao; Deng, Qilong; Li, Shanggeng; Chen, Meizhen; Jin, Bingxin; Wang, Mingcang

doi:10.1007/s12031-020-01626-4

Cited by 17 publications

(14 citation statements)

References 26 publications

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“…Recently, a study found that the expression of TRPV1 mRNA and protein was significantly up-regulated in the dorsal spinal cord tissues in a rat model of chronic constriction injury [28] . TRPV1 up-regulation apparently increased mechanical allodynia and thermal hyperalgesia as well as the expression of inflammationassociated genes (COX-2, TNF-α, and IL-6).…”

Section: Trpv1 Channelmentioning

confidence: 99%

“…Moreover, TRPV1 was identified as a downstream target of miR-338-3p and overexpression of TRPV1 restrained the inhibitory effect of miR-338-3p on neuropathic. It appears that TRPV1 has the potential to be a target for the treatment of neuropathic [28] . Clearly, further studies on this pathway may yield desirable results for the development of novel therapeutic strategy of migraine, a typical neuroinflammatory disease.…”

Section: Trpv1 Channelmentioning

confidence: 99%

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Advances on pathogenesis of migraine in cold regions

Chen

Pan

Yao

et al. 2022

Frigid Zone Medicine

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Migraine is a common primary headache which seriously affects the quality of patients’ life due to the high prevalence and disability rate. Recent years a large number of studies have found that temperature is directly bound to migraine and migraine patients in cold regions have higher prevalence, different manifestations and poor response to the conventional therapy. We propose in this review article a new concept of migraine in cold regions on the basis of geography and summarize the research advances on the pathogenesis of migraine in cold regions to provide conceptual basis for the clinical diagnosis and treatment of this disease entity.

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Section: Trpv1 Channelmentioning

confidence: 99%

Section: Trpv1 Channelmentioning

confidence: 99%

Advances on pathogenesis of migraine in cold regions

Chen

Pan

Yao

et al. 2022

Frigid Zone Medicine

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“…More recently, several studies examined the regulatory role played by microRNAs (miRNAs) in posttranscriptional regulation of TRPV1. For instance, it was demonstrated that miR-338-3p directly targets TRPV1, increasing mechanical allodynia and thermal hyperalgesia as well as the expression of inflammation-associated genes (COX-2, TNF-α, and IL-6) [80]. Regarding the spermatozoa, dysregulation of miRNAs has been correlated to male infertility [170], allowing to hypothesize a correlation between miRNAs and TRPV1 receptor also in spermatozoa.…”

Section: Sperm Chemotaxis Trpv1 and The Endocannabinoid System (Ecs)mentioning

confidence: 99%

“…According to the position of the first double bond present in their structures, this big family can be divided into n-3 (α-linolenic acid (ALA) and its products eicosapentanoic acid (EPA), docosahexaenoic acid (DHA), 20-hydroxyeicosapentaenoic acid (20-HEPE) and 22-hydroxyeicosapentaenoic acid (22-HDoHE)), and n-6 linoleic acid (LA) and its products γ-linoleic acid, arachidonic acid (AA) [ 69 ] (which produces hepoxylin, HXA-3, 14,15-epoxyeicosatrienoic acid, 14,15-EET, and 20-hydroxyeicosatetranoic acid, 20-HETE), 9- and 13-hydroxyoctadecadienoic acids (9-HODE and 13-HODE). Other agonists endogenously secreted are oxytocin [ 70 ], adenosine [ 71 ], nitric oxide [ 72 ], hydrogen sulfide (H 2 S) [ 73 ], lysophosphatidic acid (LPA) [ 74 ] and its derivative diacylglycerol [ 75 ], pH variations [ 76 , 77 ], vitamin D [ 78 ], N-acyl amino acids/neurotransmitters [ 79 ], miRNAs [ 80 , 81 ], prostaglandins, nerve growth factors and the endocannabinoids family, including N-Acyl amides [ 82 ] (N-acyl GABAS [ 83 , 84 ] derived then in D-GABAs, L-GABA and A-GABA) and N-acylethanolamines (NAEs) [ 85 ], such as anandamide (AEA) *** [ 86 ], oleylethanolamine (OEA) [ 87 ] and palmitoylethanolamine (PEA) [ 88 ]. * Product from arachidonic acid.…”

Section: Figurementioning

confidence: 99%

Role and Modulation of TRPV1 in Mammalian Spermatozoa: An Updated Review

Ramal-Sanchez

Bernabò

Valbonetti

et al. 2021

IJMS

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Based on the abundance of scientific publications, the polymodal sensor TRPV1 is known as one of the most studied proteins within the TRP channel family. This receptor has been found in numerous cell types from different species as well as in spermatozoa. The present review is focused on analyzing the role played by this important channel in the post-ejaculatory life of spermatozoa, where it has been described to be involved in events such as capacitation, acrosome reaction, calcium trafficking, sperm migration, and fertilization. By performing an exhaustive bibliographic search, this review gathers, for the first time, all the modulators of the TRPV1 function that, to our knowledge, were described to date in different species and cell types. Moreover, all those modulators with a relationship with the reproductive process, either found in the female tract, seminal plasma, or spermatozoa, are presented here. Since the sperm migration through the female reproductive tract is one of the most intriguing and less understood events of the fertilization process, in the present work, chemotaxis, thermotaxis, and rheotaxis guiding mechanisms and their relationship with TRPV1 receptor are deeply analyzed, hypothesizing its (in)direct participation during the sperm migration. Last, TRPV1 is presented as a pharmacological target, with a special focus on humans and some pathologies in mammals strictly related to the male reproductive system.

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“…NECAB2 has been reported to interact with two G-protein coupled receptors in the human embryonic kidney cells in a calcium-regulated manner ( Canela et al, 2007 , 2009 ). At the spinal level, necab2 is down-regulated by the peripheral nerve injury ( Zhang et al, 2014 ) and functions as a critical determinant of pro-nociceptive neurotransmission ( Zhang et al, 2018 ; Ma et al, 2021 ). A proteomic study involving yeast two-hybrid screening showed that NECAB2 interacts with five autism-risk genes ( FMR1 , FXR1 , FXR2 , SMARCA2 , and TSC1 ) ( Sakai et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Autism-Risk Gene necab2 Regulates Psychomotor and Social Behavior as a Neuronal Modulator of mGluR1 Signaling

Chen

Long

Guo

et al. 2022

Front. Mol. Neurosci.

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BackgroundDe novo deletion of the neuronal calcium-binding protein 2 (NECAB2) locus is associated with idiopathic autism spectrum disorders (ASDs). The in vivo function of NECAB2 in the brain remains largely elusive.MethodsWe investigated the morphological and behavioral profiles of both necab2 knock-out and overexpression zebrafish models. The expression pattern and molecular role of necab2 were probed through a combination of in vitro and in vivo assays.ResultsWe show that Necab2 is a neuronal specific, cytoplasmic, and membrane-associated protein, abundantly expressed in the telencephalon, habenula, and cerebellum. Necab2 is distributed peri-synaptically in subsets of glutamatergic and GABAergic neurons. CRISPR/Cas9-generated necab2 knock-out zebrafish display normal morphology but exhibit a decrease in locomotor activity and thigmotaxis with impaired social interaction only in males. Conversely, necab2 overexpression yields behavioral phenotypes opposite to the loss-of-function. Proteomic profiling uncovers a role of Necab2 in modulating signal transduction of G-protein coupled receptors. Specifically, co-immunoprecipitation, immunofluorescence, and confocal live-cell imaging suggest a complex containing NECAB2 and the metabotropic glutamate receptor 1 (mGluR1). In vivo measurement of phosphatidylinositol 4,5-bisphosphate further substantiates that Necab2 promotes mGluR1 signaling.ConclusionsNecab2 regulates psychomotor and social behavior via modulating a signaling cascade downstream of mGluR1.

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TRPV1, Targeted by miR-338-3p, Induces Neuropathic Pain by Interacting with NECAB2

Cited by 17 publications

References 26 publications

Advances on pathogenesis of migraine in cold regions

Advances on pathogenesis of migraine in cold regions

Role and Modulation of TRPV1 in Mammalian Spermatozoa: An Updated Review

Autism-Risk Gene necab2 Regulates Psychomotor and Social Behavior as a Neuronal Modulator of mGluR1 Signaling

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